In-Vivo High-Resolution Diffuse Luminescence Molecular Imaging using Rare-Earth Doped Upconverting Nanoparticles as Contrast Agents

2013 ◽  
Author(s):  
Can Xu ◽  
Haichun Liu ◽  
Anna Gisselsson ◽  
Pontus Svenmarker ◽  
Nina Rogelius ◽  
...  
Keyword(s):  
Rare Earth ◽  
Molecular Imaging ◽  
High Resolution ◽  
Contrast Agents ◽  
Rare Earth Doped

Near-infrared (1550 nm) in vivo bioimaging based on rare-earth doped ceramic nanophosphors modified with PEG-b-poly(4-vinylbenzylphosphonate)

Nanoscale ◽  
2011 ◽  
Vol 3 (9) ◽  
pp. 3705 ◽  
Author(s):  
Masao Kamimura ◽  
Naoki Kanayama ◽  
Kimikazu Tokuzen ◽  
Kohei Soga ◽  
Yukio Nagasaki
Keyword(s):  
Rare Earth ◽  
Near Infrared ◽  
Rare Earth Doped

scholarly journals Molecular imaging perspectives

2005 ◽  
Vol 2 (3) ◽  
pp. 133-144 ◽  
Author(s):  
Paul J Cassidy ◽  
George K Radda
Keyword(s):  
Molecular Imaging ◽  
Contrast Agents ◽  
Physical Science ◽  
Life Science ◽  
Interdisciplinary Collaboration ◽  
Nuclear Imaging ◽  
Imaging Modalities ◽  
Molecular Events ◽  
Biological Target

Molecular imaging is an emerging technology at the life science/physical science interface which is set to revolutionize our understanding and treatment of disease. The tools of molecular imaging are the imaging modalities and their corresponding contrast agents. These facilitate interaction with a biological target at a molecular level in a number of ways. The diverse nature of molecular imaging requires knowledge from both the life and physical sciences for its successful development and implementation. The aim of this review is to introduce the subject of molecular imaging from both life science and physical science perspectives. However, we will restrict our coverage to the prominent in vivo molecular imaging modalities of magnetic resonance imaging, optical imaging and nuclear imaging. The physical basis of these imaging modalities, the use of contrast agents and the imaging parameters of sensitivity, temporal resolution and spatial resolution are described. Then, the specificity of contrast agents for targeting and sensing molecular events, and some applications of molecular imaging in biology and medicine are given. Finally, the diverse nature of molecular imaging and its reliance on interdisciplinary collaboration is discussed.

Anti-Biofouling Polymer-Decorated Lutetium-Based Nanoparticulate Contrast Agents for In Vivo High-Resolution Trimodal Imaging

Small ◽  
2014 ◽  
Vol 10 (12) ◽  
pp. 2429-2438 ◽  
Author(s):  
Zhen Liu ◽  
Kai Dong ◽  
Jianhua Liu ◽  
Xueli Han ◽  
Jinsong Ren ◽  
...  
Keyword(s):  
High Resolution ◽  
Contrast Agents

Engineering the Design of Brightly-Emitting Luminescent Nanostructured Photonic Composite Systems

2013 ◽  
Vol 66 (9) ◽  
pp. 1008 ◽  
Author(s):  
Mei Chee Tan ◽  
Dominik J. Naczynski ◽  
Prabhas V. Moghe ◽  
Richard E. Riman
Keyword(s):  
Rare Earth ◽  
Optical Waveguides ◽  
Impact Resistance ◽  
Synthesis Method ◽  
Research Progress ◽  
Design And Synthesis ◽  
Composite Systems ◽  
Rare Earth Doped

Rare-earth doped infrared emitting composites have extensive applications in integrated optical devices such as fibre amplifiers and waveguides for telecommunications, remote sensing, and optoelectronics. In addition, recent advancements in infrared optical imaging systems have expanded the biomedical applications for infrared-emitting composites in diagnosis and imaging of living tissue systems both in vitro and in vivo. Composite systems combine the advantages of polymers (light weight, flexibility, good impact resistance, improved biomedical compatibility, and excellent processability) and inorganic phosphor host materials (low phonon energy, intense emissions, chemical durability, and high thermal stability). This paper provides a brief review of our research progress in the design and synthesis of luminescent photonic nanocomposite systems comprised of rare-earth doped particulates dispersed in a continuous polymeric matrix. The design of brightly-emitting rare-earth doped materials and the influence of host and dopant chemistries on the emission properties are discussed. Methods used to assess and measure the phosphors’ performance are also evaluated in this work. This paper will also examine the solvothermal synthesis method used to control the physical and chemical characteristics of the rare-earth doped particles, and how these characteristics impact the infrared optical properties. Also presented here are recent advances reported with luminescent nanocomposite systems fabricated for optical waveguides and biomedical imaging.

Abstract 14935: Targeted Optical Molecular Imaging of Atheroma Calcification Using Novel Aldendronate-based Probe

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Dong Oh Kang ◽  
Yong Geun Lim ◽  
Joon Woo Song ◽  
Ye Hee Park ◽  
Hyun Jung Kim ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
High Resolution ◽  
Murine Model ◽  
Calcium Binding ◽  
Laser Scanning ◽  
Near Infrared ◽  
Imaging System ◽  
Whole Body ◽  
Imaging Results

Background/Objectives: Vascular spotty calcification is an actively regulated biological process resulting in plaque vulnerability. We investigated the feasibility of a novel alendronate-based near-infrared fluorescence (NIRF)-emitting probe to specifically target atherosclerotic calcification in a murine model in vivo using our customized high-resolution multichannel intravital molecular imaging system (IVFM). Methods/Results: We have fabricated a calcium-binding NIRF probe by chemically coupling alendronate, a specific targeting ligand, and NIRF-emitting Cy5.5 to the ends of azide-PEG-NHS ester (Al-Cy5.5). Prepared Al-Cy5.5 has high affinity for calcium phosphate-containing bone minerals. In vitro, Al-Cy5.5 specifically binds to RANKL-induced osteogenic-macrophages as compared to macrophages (p<0.01). On whole body fluorescence imaging to assess time-dependent excretion, NIRF signals remained visible up to 48 hrs. Then, in mice with calcified plaque induced by a combination diet of high-cholesterol and warfarin, Al-Cy5.5 (2.5 mg/kg) was intravenously injected. 48 hrs after administration, murine calcified atheroma was assessed using a customized high-resolution multichannel IVFM, which demonstrated highly enhanced NIRF signals in vivo in the calcified areas of murine carotid plaques (p<0.01, Figure). Ex vivo laser scanning fluorescence microscopic and immune-histological findings from the corresponding sister sections well corroborated the in vivo imaging results, which demonstrated the co-localization of NIRF signals with plaque calcifications (von-Kossa stain). Conclusions: Our novel calcification targeted probe, Al-Cy5.5, was able to selectively target atheroma calcification in vivo in a murine model as assessed by optical IVFM. This novel targetable strategy is expected to provide a promising theranostic basis for calcified high-risk plaques by integration with multimodal customized catheter imaging system.

scholarly journals Rare-earth-doped biological composites as in vivo shortwave infrared reporters

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
D. J. Naczynski ◽  
M. C. Tan ◽  
M. Zevon ◽  
B. Wall ◽  
J. Kohl ◽  
...  
Keyword(s):  
Rare Earth ◽  
Biological Composites ◽  
Shortwave Infrared ◽  
Rare Earth Doped

DEVELOPMENT OF NEEDLE-BASED MICROENDOSCOPY FOR FLUORESCENCE MOLECULAR IMAGING OF BREAST TUMOR MODELS

2009 ◽  
Vol 02 (04) ◽  
pp. 343-352
Author(s):  
CHAO-WEI CHEN ◽  
TIFFANY R. BLACKWELL ◽  
RENEE NAPHAS ◽  
PAUL T. WINNARD ◽  
VENU RAMAN ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Molecular Imaging ◽  
Contrast Agents ◽  
Breast Biopsy ◽  
Fluorescent Imaging ◽  
Model Systems ◽  
Breast Cancers ◽  
Imaging Device ◽  
Fluorescence Molecular Imaging

Fluorescence molecular imaging enables the visualization of basic molecular processes such as gene expression, enzyme activity, and disease-specific molecular interactions in vivo using targeted contrast agents, and therefore, is being developed for early detection and in situ characterization of breast cancers. Recent advances in developing near-infrared fluorescent imaging contrast agents have enabled the specific labeling of human breast cancer cells in mouse model systems. In synergy with contrast agent development, this paper describes a needle-based fluorescence molecular imaging device that has the strong potential to be translated into clinical breast biopsy procedures. This microendoscopy probe is based on a gradient-index (GRIN) lens interfaced with a laser scanning microscope. Specifications of the imaging performance, including the field-of-view, transverse resolution, and focus tracking characteristics were calibrated. Orthotopic MDA-MB-231 breast cancer xenografts stably expressing the tdTomato red fluorescent protein (RFP) were used to detect the tumor cells in this tumor model as a proof of principle study. With further development, this technology, in conjunction with the development of clinically applicable, injectable fluorescent molecular imaging agents, promises to perform fluorescence molecular imaging of breast cancers in vivo for breast biopsy guidance.

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