Anti-Biofouling Polymer-Decorated Lutetium-Based Nanoparticulate Contrast Agents for In Vivo High-Resolution Trimodal Imaging

Small ◽  
2014 ◽  
Vol 10 (12) ◽  
pp. 2429-2438 ◽  
Author(s):  
Zhen Liu ◽  
Kai Dong ◽  
Jianhua Liu ◽  
Xueli Han ◽  
Jinsong Ren ◽  
...  
Keyword(s):  
High Resolution ◽  
Contrast Agents

scholarly journals Biodegradable harmonophores for targeted high-resolution in vivo tumor imaging

2019 ◽  
Author(s):  
Ali Yasin Sonay ◽  
Sine Yaganoglu ◽  
Martina Konantz ◽  
Claire Teulon ◽  
Sandro Sieber ◽  
...  
Keyword(s):  
High Resolution ◽  
Contrast Agents ◽  
Optical Imaging ◽  
Tumor Imaging ◽  
Second Harmonic ◽  
Detection Sensitivity ◽  
Great Promise ◽  
Imaging Probes

AbstractOptical imaging probes have played a major role in detecting and monitoring of a variety of diseases1. In particular, nonlinear optical imaging probes, such as second harmonic generating (SHG) nanoprobes, hold great promise as clinical contrast agents, as they can be imaged with little background signal and unmatched long-term photostability2. As their chemical composition often includes transition metals, the use of inorganic SHG nanoprobes can raise long-term health concerns. Ideally, contrast agents for biomedical applications should be degraded in vivo without any long-term toxicological consequences to the organism. Here, we developed biodegradable harmonophores (bioharmonophores) that consist of polymer-encapsulated, self-assembling peptides that generate a strong SHG signal. When functionalized with tumor cell surface markers, these reporters can target single cancer cells with high detection sensitivity in zebrafish embryos in vivo. Thus, bioharmonophores will enable an innovative approach to cancer treatment using targeted high-resolution optical imaging for diagnostics and therapy.

High-resolution functional imaging with ultrasound contrast agents based on RF processing in an in vivo kidney experiment

2001 ◽  
Vol 27 (2) ◽  
pp. 223-233 ◽  
Author(s):  
Xander A.A.M Verbeek ◽  
Jean M Willigers ◽  
Frits W Prinzen ◽  
Maaike Peschar ◽  
Lón A.F Ledoux ◽  
...  
Keyword(s):  
High Resolution ◽  
Contrast Agents ◽  
Functional Imaging ◽  
Ultrasound Contrast Agents ◽  
Ultrasound Contrast

Tantalum Oxide Nanoparticles as Versatile Contrast Agents for X-ray Computed Tomography

2020 ◽  
Author(s):  
Shatadru Chakravarty ◽  
Jeremy Hix ◽  
Kaitlyn Wieweora ◽  
Maximilian Volk ◽  
Elizabeth Kenyon ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Contrast Agents ◽  
Mesoporous Silica Nanoparticles ◽  
Sol Gel ◽  
Tantalum Oxide ◽  
High Signal ◽  
Drug Delivery Vehicles ◽  
X Ray Computed

Here we describe the synthesis, characterization and in vitro and in vivo performance of a series of tantalum oxide (TaOx) based nanoparticles (NPs) for computed tomography (CT). Five distinct versions of 9-12 nm diameter silane coated TaOx nanocrystals (NCs) were fabricated by a sol-gel method with varying degrees of hydrophilicity and with or without fluorescence, with the highest reported Ta content to date (78%). Highly hydrophilic NCs were left bare and were evaluated in vivo in mice for micro-CT of full body vasculature, where following intravenous injection, TaOx NCs demonstrate high CT contrast, circulation in blood for ~ 3 h, and eventual accumulation in RES organs; and following injection locally in the mammary gland, where the full ductal tree structure can be clearly delineated. Partially hydrophilic NCs were encapsulated within mesoporous silica nanoparticles (MSNPs; TaOx@MSNPs) and hydrophobic NCs were encapsulated within poly(lactic-co-glycolic acid) (PLGA; TaOx@PLGA) NPs, serving as potential CT-imagable drug delivery vehicles. Bolus intramuscular injections of TaOx@PLGA NPs and TaOx@MSNPs to mimic the accumulation of NPs at a tumor site produce high signal enhancement in mice. In vitro studies on bare NCs and formuated NPs demonstrate high cytocompatibility and low dissolution of TaOx. This work solidifies that TaOx-based NPs are versatile contrast agents for CT.

Rapid Catalyst Capture Enables Metal-Free Parahydrogen-Based Hyperpolarized Contrast Agents

2018 ◽  
Author(s):  
Danila Barskiy ◽  
Lucia Ke ◽  
Xingyang Li ◽  
Vincent Stevenson ◽  
Nevin Widarman ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Contrast Agents ◽  
Inductively Coupled Plasma ◽  
Organometallic Compounds ◽  
Atomic Emission ◽  
Platinum Group Metals ◽  
Resonance Spectroscopy ◽  
Plasma Atomic Emission ◽  
Inductively Coupled

<p>Hyperpolarization techniques based on the use of parahydrogen provide orders of magnitude signal enhancement for magnetic resonance spectroscopy and imaging. The main drawback limiting widespread applicability of parahydrogen-based techniques in biomedicine is the presence of organometallic compounds (the polarization transfer catalysts) in solution with hyperpolarized contrast agents. These catalysts are typically complexes of platinum-group metals and their administration in vivo should be avoided.</p> <p><br></p><p>Herein, we show how extraction of a hyperpolarized compound from an organic phase to an aqueous phase combined with a rapid (less than 10 seconds) Ir-based catalyst capture by metal scavenging agents can produce pure parahydrogen-based hyperpolarized contrast agents as demonstrated by high-resolution nuclear magnetic resonance (NMR) spectroscopy and inductively coupled plasma atomic emission spectroscopy (ICP-AES). The presented methodology enables fast and efficient means of producing pure hyperpolarized aqueous solutions for biomedical and other uses.</p>

Rapid Catalyst Capture Enables Metal-Free Parahydrogen-Based Hyperpolarized Contrast Agents

2018 ◽  
Author(s):  
Danila Barskiy ◽  
Lucia Ke ◽  
Xingyang Li ◽  
Vincent Stevenson ◽  
Nevin Widarman ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Contrast Agents ◽  
Inductively Coupled Plasma ◽  
Organometallic Compounds ◽  
Atomic Emission ◽  
Platinum Group Metals ◽  
Resonance Spectroscopy ◽  
Plasma Atomic Emission ◽  
Inductively Coupled

<p>Hyperpolarization techniques based on the use of parahydrogen provide orders of magnitude signal enhancement for magnetic resonance spectroscopy and imaging. The main drawback limiting widespread applicability of parahydrogen-based techniques in biomedicine is the presence of organometallic compounds (the polarization transfer catalysts) in solution with hyperpolarized contrast agents. These catalysts are typically complexes of platinum-group metals and their administration in vivo should be avoided.</p> <p><br></p><p>Herein, we show how extraction of a hyperpolarized compound from an organic phase to an aqueous phase combined with a rapid (less than 10 seconds) Ir-based catalyst capture by metal scavenging agents can produce pure parahydrogen-based hyperpolarized contrast agents as demonstrated by high-resolution nuclear magnetic resonance (NMR) spectroscopy and inductively coupled plasma atomic emission spectroscopy (ICP-AES). The presented methodology enables fast and efficient means of producing pure hyperpolarized aqueous solutions for biomedical and other uses.</p>

Supramolecular Enhancement of Aminoxyl ORCA Contrast Agents

2019 ◽  
Author(s):  
Hamilton Lee ◽  
Jenica Lumata ◽  
Michael A. Luzuriaga ◽  
Candace Benjamin ◽  
Olivia Brohlin ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Side Effects ◽  
Magnetic Resonance ◽  
Tobacco Mosaic Virus ◽  
Contrast Agents ◽  
Single Molecule ◽  
Organic Radical ◽  
Resonance Imaging ◽  
Physiological Conditions

<div><div><div><p>Many contrast agents for magnetic resonance imaging are based on gadolinium, however side effects limit their use in some patients. Organic radical contrast agents (ORCAs) are potential alternatives, but are reduced rapidly in physiological conditions and have low relaxivities as single molecule contrast agents. Herein, we use a supramolecular strategy where cucurbit[8]uril binds with nanomolar affinities to ORCAs and protects them against biological reductants to create a stable radical in vivo. We further over came the weak contrast by conjugating this complex on the surface of a self-assembled biomacromolecule derived from the tobacco mosaic virus.</p></div></div></div>

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