Development and characterization of disposable sub-millimeter fiberoptic Raman needle probe for real-time in vivo deep tissue and biofluids Raman measurements

2021 ◽  
Author(s):  
Zhiwei Huang ◽  
Chi Shu ◽  
Wei Zheng ◽  
Zhuo Wang ◽  
Changyuan Yu
Keyword(s):  
Real Time ◽  
Deep Tissue ◽  
Needle Probe

Real-Time In Vivo Characterization of Primary Liver Tumors With Diffuse Optical Spectroscopy During Percutaneous Needle Interventions

2015 ◽  
Vol 50 (7) ◽  
pp. 443-448 ◽  
Author(s):  
Rami Nachabé ◽  
Benno H.W. Hendriks ◽  
Ross Schierling ◽  
Jasmine Hales ◽  
Judy M. Racadio ◽  
...  
Keyword(s):  
Real Time ◽  
Optical Spectroscopy ◽  
Liver Tumors ◽  
Diffuse Optical Spectroscopy ◽  
Primary Liver Tumors ◽  
In Vivo Characterization ◽  
Needle Interventions

In vivo real-time confocal laser scanning endomicroscopic colonoscopy for the detection and characterization of colorectal neoplasia

2008 ◽  
Vol 95 (5) ◽  
pp. 636-645 ◽  
Author(s):  
D. P. Hurlstone ◽  
W. Baraza ◽  
S. Brown ◽  
M. Thomson ◽  
N. Tiffin ◽  
...  
Keyword(s):  
Real Time ◽  
Laser Scanning ◽  
Colorectal Neoplasia ◽  
Confocal Laser Scanning ◽  
Confocal Laser

scholarly journals Characterization of PEBBLEs as a Tool for Real-Time Measurement ofDictyostelium discoideumEndosomal pH

2009 ◽  
Vol 2009 ◽  
pp. 1-4 ◽  
Author(s):  
Everett Moding ◽  
Jessica Hellyer ◽  
Kevin Rank ◽  
Phoebe Lostroh ◽  
Murphy Brasuel
Keyword(s):  
Real Time ◽  
Fluorescent Dyes ◽  
Ion Concentration ◽  
Ph Sensing ◽  
Cellular Mechanisms ◽  
Real Time Measurement ◽  
Endosomal Ph ◽  
Camp Stimulation

The measurement of intracellular ion concentration change is important for understanding the cellular mechanisms for communication. Recently developed nanosensors, (Photonic Explorers for Biomedical use with Biologically Localized Embedding) PEBBLEs, have a number of advantages for measuring ions in cells over established methods using microelectrodes, unbound fluorescent dyes, or NMR. PEBBLE sensors have been shown to work in principle for measuring dynamic ion changes, but few in vivo applications have been demonstrated. We modified the protocol for the fabrication of pH sensing PEBBLEs and developed a protocol for the utilization of these sensors for the monitoring of dynamic pH changes in the endosomes of slime moldDictyostelium discoideum (D. discoideum). Oregon Green 514-CdSe Quantum Dot PEBBLEs were used to measure real-time pH insideD. discoideumendosomes during cAMP stimulation. Endosomal pH was shown to decrease during cAMP signaling, demonstrating a movement of protons into the endosomes ofD. discoideumamoebae.

Noninvasive and real‐time in vivo characterization of Inflammation skin. A feasibility of animal study

2021 ◽  
Author(s):  
Thien Luan Phan ◽  
Nguyen Van Hieu ◽  
Tzong Shiun Li ◽  
Ko‐Chang Tsao ◽  
Congo Tak Shing Ching
Keyword(s):  
Real Time ◽  
Animal Study ◽  
In Vivo Characterization

Ablation of Red Stable Transfected Prostate Cancer Cell Lines by C-CPE Gold-Nanoparticle Mediated Laser Intervention

2021 ◽  
Author(s):  
Suhayla Alnajjar ◽  
Ingo Nolte ◽  
Annegret Becker ◽  
Tina Kostka ◽  
Jan Torben Schille ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Gold Nanoparticle ◽  
Cell Lines ◽  
Cancer Cell ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Deep Tissue Imaging

Abstract Background: Claudin (CLDN) proteins have been described to be found and accordingly targeted to evaluate novel therapeutic approaches. C-terminus of Clostridium perfringens enterotoxin (C-CPE) binds efficiently several claudins and thus recombinant C-CPE conjugated to gold nanoparticles (AuNPs) has been used for cancer cell targeting using gold nanoparticle- mediated laser perforation (GNOME-LP). Cancer cells inoculation is routinely used to generate in vivo models to evaluate novel therapeutic approaches in prostate cancer. However, detailed characterization of cancer spreading and early tumor development and therapeutic response is often limited as conventional cell lines do not allow advanced deep tissue imaging.Methods: two canine prostate cancer cell lines were stably transfected with red fluorescent protein (RFP), followed by G418 selection. RFP marker as well as CLDN3, -4 and -7 expression was comparatively confirmed by flow cytometry, qPCR and immunofluorescences. For cancer cells targeting, GNOME-LP at a laser fluence of 72 mJ/cm² and a scanning speed of 0.5 cm/s was used. Statistical analysis was performed using SAS software 7.1, Dunnett´s Multiple Comparison Test and Student´s two-sided t-test. Differences were considered statistically significant for p<0.05.Results: we established two canine prostate carcinoma cell lines, stably expressing RFP allowing perspective deep tissue imaging. Directed C-CPE-AuNP binding to native and RFP transfected cells verified the capability to specifically target CLDN receptors. Cancer cell ablation was demonstrated in vitro setting using a combination of gold nanoparticle mediated laser perforation and C-CPE-AuNPs treatment reducing tumor cell viability to less than 10 % depending on cell line. Conclusion: the results confirm that this therapeutic approach can be used efficiently to target prostate carcinoma cells carrying a marker protein allowing deep tissue imaging. The established cell lines and the verified proof of concept in vitro study provide the basis for perspective Xenograft model in vivo studies. The introduce red fluorescence enables deep tissue imaging in living animals and therefore detailed characterization of tumor growth and subsequently possible tumor ablation through C-CPE-AuNPs treatment.

Sign in / Sign up

Export Citation Format

Share Document