scholarly journals Characterization of PEBBLEs as a Tool for Real-Time Measurement ofDictyostelium discoideumEndosomal pH

2009 ◽  
Vol 2009 ◽  
pp. 1-4 ◽  
Author(s):  
Everett Moding ◽  
Jessica Hellyer ◽  
Kevin Rank ◽  
Phoebe Lostroh ◽  
Murphy Brasuel
Keyword(s):  
Real Time ◽  
Fluorescent Dyes ◽  
Ion Concentration ◽  
Ph Sensing ◽  
Cellular Mechanisms ◽  
Real Time Measurement ◽  
Endosomal Ph ◽  
Camp Stimulation

The measurement of intracellular ion concentration change is important for understanding the cellular mechanisms for communication. Recently developed nanosensors, (Photonic Explorers for Biomedical use with Biologically Localized Embedding) PEBBLEs, have a number of advantages for measuring ions in cells over established methods using microelectrodes, unbound fluorescent dyes, or NMR. PEBBLE sensors have been shown to work in principle for measuring dynamic ion changes, but few in vivo applications have been demonstrated. We modified the protocol for the fabrication of pH sensing PEBBLEs and developed a protocol for the utilization of these sensors for the monitoring of dynamic pH changes in the endosomes of slime moldDictyostelium discoideum (D. discoideum). Oregon Green 514-CdSe Quantum Dot PEBBLEs were used to measure real-time pH insideD. discoideumendosomes during cAMP stimulation. Endosomal pH was shown to decrease during cAMP signaling, demonstrating a movement of protons into the endosomes ofD. discoideumamoebae.

Direct real-time measurement of in vivo forces in the lumbar spine

2005 ◽  
Vol 5 (1) ◽  
pp. 85-94 ◽  
Author(s):  
Eric H. Ledet ◽  
Michael P. Tymeson ◽  
Darryl J. DiRisio ◽  
Benjamin Cohen ◽  
Richard L. Uhl
Keyword(s):  
Lumbar Spine ◽  
Real Time ◽  
Time Measurement ◽  
Real Time Measurement

scholarly journals Ratiometric pH Sensing and Imaging in Living Cells with Dual-Emission Semiconductor Polymer Dots

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2923
Author(s):  
Piaopiao Chen ◽  
Iqra Ilyas ◽  
Su He ◽  
Yichen Xing ◽  
Zhigang Jin ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescein Isothiocyanate ◽  
Water Soluble ◽  
Ph Sensing ◽  
Dual Emission ◽  
Polymer Dots ◽  
Ratiometric Ph Sensing

Polymer dots (Pdots) represent newly developed semiconductor polymer nanoparticles and exhibit excellent characteristics as fluorescent probes. To improve the sensitivity and biocompatibility of Pdots ratiometric pH biosensors, we synthesized 3 types of water-soluble Pdots: Pdots-PF, Pdots-PP, and Pdots-PPF by different combinations of fluorescent dyes poly(9,9-dioctylfluorenyl-2,7-diyl) (PFO), poly[(9,9-dioctyl-fluorenyl-2,7-diyl)-co-(1,4-benzo-{2,1′,3}-thiadazole)] (PFBT), and fluorescein isothiocyanate (FITC). We found that Pdots-PPF exhibits optimal performance on pH sensing. PFO and FITC in Pdots-PPF produce pH-insensitive (λ = 439 nm) and pH-sensitive (λ = 517 nm) fluorescence respectively upon a single excitation at 380 nm wavelength, which enables Pdots-PPF ratiometric pH sensing ability. Förster resonance energy transfer (FRET) together with the use of PFBT amplify the FITC signal, which enables Pdots-PPF robust sensitivity to pH. The emission intensity ratio (I517/I439) of Pdots-PPF changes linearly as a function of pH within the range of pH 3.0 to 8.0. Pdots-PPF also possesses desirable reversibility and stability in pH measurement. More importantly, Pdots-PPF was successfully used for cell imaging in Hela cells, exhibiting effective cellular uptake and low cytotoxicity. Our study suggests the promising potential of Pdots-PPF as an in vivo biomarker.

scholarly journals Loading Graphene Quantum Dots into Optical-Magneto Nanoparticles for Real-Time Tracking In Vivo

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2191 ◽  
Author(s):  
Yu Wang ◽  
Nan Xu ◽  
Yongkai He ◽  
Jingyun Wang ◽  
Dan Wang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Real Time ◽  
Fluorescent Dyes ◽  
Graphene Quantum Dots ◽  
Shell Nanoparticles ◽  
One Pot ◽  
Real Time Tracking

Fluorescence imaging offers a new approach to visualize real-time details on a cellular level in vitro and in vivo without radioactive damage. Poor light stability of organic fluorescent dyes makes long-term imaging difficult. Due to their outstanding optical properties and unique structural features, graphene quantum dots (GQDs) are promising in the field of imaging for real-time tracking in vivo. At present, GQDs are mainly loaded on the surface of nanoparticles. In this study, we developed an efficient and convenient one-pot method to load GQDs into nanoparticles, leading to longer metabolic processes in blood and increased delivery of GQDs to tumors. Optical-magneto ferroferric oxide@polypyrrole (Fe3O4@PPy) core-shell nanoparticles were chosen for their potential use in cancer therapy. The in vivo results demonstrated that by loading GQDs, it was possible to monitor the distribution and metabolism of nanoparticles. This study provided new insights into the application of GQDs in long-term in vivo real-time tracking.

Real-Time In Vivo Characterization of Primary Liver Tumors With Diffuse Optical Spectroscopy During Percutaneous Needle Interventions

2015 ◽  
Vol 50 (7) ◽  
pp. 443-448 ◽  
Author(s):  
Rami Nachabé ◽  
Benno H.W. Hendriks ◽  
Ross Schierling ◽  
Jasmine Hales ◽  
Judy M. Racadio ◽  
...  
Keyword(s):  
Real Time ◽  
Optical Spectroscopy ◽  
Liver Tumors ◽  
Diffuse Optical Spectroscopy ◽  
Primary Liver Tumors ◽  
In Vivo Characterization ◽  
Needle Interventions

Synthesis and Characterization of Ratiometric, pH Sensing Nanoparticles with Covalently Attached Fluorescent Dyes

2006 ◽  
Vol 18 (15) ◽  
pp. 3381-3384 ◽  
Author(s):  
Honghao Sun ◽  
Anne Marie Scharff-Poulsen ◽  
Hong Gu ◽  
Kristoffer Almdal
Keyword(s):  
Fluorescent Dyes ◽  
Synthesis And Characterization ◽  
Ph Sensing ◽  
Ratiometric Ph Sensing
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