In vivo real-time confocal laser scanning endomicroscopic colonoscopy for the detection and characterization of colorectal neoplasia

2008 ◽  
Vol 95 (5) ◽  
pp. 636-645 ◽  
Author(s):  
D. P. Hurlstone ◽  
W. Baraza ◽  
S. Brown ◽  
M. Thomson ◽  
N. Tiffin ◽  
...  
Keyword(s):  
Real Time ◽  
Laser Scanning ◽  
Colorectal Neoplasia ◽  
Confocal Laser Scanning ◽  
Confocal Laser

In vivo confocal laser scanning chromo-endomicroscopy of colorectal neoplasia: changing the technological paradigm

2008 ◽  
Vol 52 (4) ◽  
pp. 417-426 ◽  
Author(s):  
D P Hurlstone ◽  
N Tiffin ◽  
S R Brown ◽  
W Baraza ◽  
M Thomson ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Colorectal Neoplasia ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Technological Paradigm

Real-time in-vivo imaging of pulmonary capillary perfusion using probe-based confocal laser scanning endomicroscopy in pigs

2015 ◽  
Vol 32 (6) ◽  
pp. 392-399 ◽  
Author(s):  
Sybille Gruber ◽  
Isabella Spielauer ◽  
Stefan Böhme ◽  
David Baron ◽  
Klaus Markstaller ◽  
...  
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Laser Scanning ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Capillary Perfusion ◽  
Pulmonary Capillary

Probing Enhanced Cytochrome P450 2B1/2 Activity in Rat Hepatocyte Spheroids through Confocal Laser Scanning Microscopy

2001 ◽  
Vol 10 (3) ◽  
pp. 329-342 ◽  
Author(s):  
Emmanouhl S. Tzanakakis ◽  
Chang-Chun Hsiao ◽  
Taku Matsushita ◽  
Rory P. Remmel ◽  
Wei-Shou Hu
Keyword(s):  
Cytochrome P450 ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Constitutive Expression ◽  
Rat Hepatocytes ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser

Cytochrome P450 (CYP450) enzymes are essential for xenobiotic metabolism. Although CYP450s are found in many tissues, CYP2B1/2 are primarily expressed in the rat liver. The constitutive expression in vivo of CYP2B1/2 is low but it is induced in the presence of various drugs such as phenobarbital (PB). In this study, CYP2B1/2 activity in cultured hepatocytes was assessed in situ with the introduction of a fluorogenic sub-strate, pentoxyresorufin. The product of 7-pentoxyresorufin-O-dealkylation (PROD), which is catalyzed specifically by CYP2B1/2, was detected using confocal laser scanning microscopy (CLSM). Primary hepatocytes cultured as monolayers on collagen-coated surfaces exhibited background PROD activity and minimal PB inducibility after 4 days in culture. In contrast, rat hepatocytes organized in compacted aggregates, or spheroids, exhibited higher levels of PROD activity and retained their ability for PB induction. The results from the CLSM analysis were verified by RT-PCR and Western immunoblotting analysis. Furthermore, CLSM in conjunction with image processing techniques and three-dimensional reconstruction revealed the localization of enhanced PROD activity in the center of spheroids. The results support the use of CLSM as a powerful tool for investigating CYP2B1/2 activity in cultured rat hepatocytes.

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