Rapid determination of the three-dimensional orientation of single molecules

2001 ◽  
Vol 26 (4) ◽  
pp. 211 ◽  
Author(s):  
John T. Fourkas
Keyword(s):  
Rapid Determination ◽  
Single Molecules ◽  
Three Dimensional

Structural Analysis of Proteins on Lipid Substrates

2000 ◽  
Vol 6 (S2) ◽  
pp. 1182-1183
Author(s):  
Elizabeth M. Wilson-Kubalek
Keyword(s):  
Structural Information ◽  
Rapid Determination ◽  
3D Structure ◽  
Three Dimensional ◽  
Molecular Complexes ◽  
Structural Data ◽  
X Ray Crystallography ◽  
Helical Protein ◽  
3D Structure Determination

Electron microscopy (EM) has become an increasingly powerful method for the determination of three-dimensional (3D) structures of proteins and macromolecular complexes. EM offers advantages over X-ray crystallography and NMR for obtaining structural information about proteins in physiological conditions, as components of large assemblies, that cannot be obtained in large quantity, or that fail to yield 3D crystals. EM has been used to obtain structural data from images of isolated molecules and molecular complexes, two-dimensional (2D) protein crystals, and helical protein arrays. Helically arranged proteins allow the most rapid determination of 3D maps because they contain a complete range of equally spaced molecular views, therefore no tilting of the sample with respect to the electron beam is required. However, so far 3D structure determination of helical assemblies has been limited to proteins that naturally adopt this organization and to proteins that fortuitously crystallize as helices.

Determination of the three-dimensional orientation of single molecules

2008 ◽  
Vol 33 (9) ◽  
pp. 1020 ◽  
Author(s):  
Matthew R. Foreman ◽  
Carlos Macías Romero ◽  
Peter Török
Keyword(s):  
Single Molecules ◽  
Three Dimensional

Application of Three-Dimensional Fluorescence Spectroscopy Coupled with ATLD in Rapid Determination of Triazophos Content in Duck Meat

2014 ◽  
Vol 651-653 ◽  
pp. 362-366
Author(s):  
Jin Hui Zhao ◽  
Hai Bin Xiao ◽  
Hai Chao Yuan ◽  
Qian Hong ◽  
Mu Hua Liu
Keyword(s):  
Fluorescence Spectroscopy ◽  
Mean Squared Error ◽  
Rapid Determination ◽  
Organic Phosphorus ◽  
Three Dimensional ◽  
Meat Extract ◽  
Trilinear Decomposition ◽  
Proposed Model ◽  
Standard Solution

The triazophos is a kind of organic phosphorus pesticide, the quantitative determination model based on three-dimensional fluorescence spectroscopy coupled with alternating trilinear decomposition (ATLD) was explored for realizing the rapid determination of triazophos content in duck meat. Firstly, three-dimensional fluorescence spectra of duck meat extract, triazophos standard solution and duck meat extract containing triazophos were explored, respectively. Secondly, the fluorescence quenching phenomenon of the triazophos in duck meat extract was analyzed. Lastly, the number of components for three linear decomposition of ATLD was set as 2 by using the core consistency diagnostic, and the calibration curve between the relative fluorescence intensity and the actual concentration of the triazophos was established by using ATLD. The experimental results showed that the determination coefficient (R2) and the root mean squared error of prediction (RMSEP) for the proposed model in this paper were 0.9741 and 0.764 respectively, and it was feasible to predict the triazophos content in duck meat combining with three-demensional fluorescence spectroscopy and ATLD.

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