Simple scheme for rapid three-dimensional orientation determination of the emission dipole of single molecules

2005 ◽  
Vol 86 (12) ◽  
pp. 121104 ◽  
Author(s):  
Johannes Hohlbein ◽  
Christian G. Hübner
Keyword(s):  
Single Molecules ◽  
Three Dimensional ◽  
Simple Scheme ◽  
Orientation Determination

Orientation determination of single molecules by highly focused generalized cylindrical vector beams

2009 ◽  
Author(s):  
Xianghui Wang ◽  
Shengjiang Chang ◽  
Lie Lin ◽  
Linrui Wang ◽  
Shujuan Hao
Keyword(s):  
Single Molecules ◽  
Vector Beams ◽  
Orientation Determination

Determination of the three-dimensional orientation of single molecules

2008 ◽  
Vol 33 (9) ◽  
pp. 1020 ◽  
Author(s):  
Matthew R. Foreman ◽  
Carlos Macías Romero ◽  
Peter Török
Keyword(s):  
Single Molecules ◽  
Three Dimensional

Conformation of Ribosomes from Escherichia Coli

1974 ◽  
Vol 32 ◽  
pp. 210-211
Author(s):  
M. Boublik ◽  
W. Hellmann ◽  
F. Jenkins
Keyword(s):  
Binding Sites ◽  
Ribosomal Proteins ◽  
Fluorescent Dyes ◽  
Protein Biosynthesis ◽  
Three Dimensional ◽  
Spatial Arrangement ◽  
Dimensional Structure ◽  
Complete Understanding ◽  
And Function

The present knowledge of the three-dimensional structure of ribosomes is far too limited to enable a complete understanding of the various roles which ribosomes play in protein biosynthesis. The spatial arrangement of proteins and ribonuclec acids in ribosomes can be analysed in many ways. Determination of binding sites for individual proteins on ribonuclec acid and locations of the mutual positions of proteins on the ribosome using labeling with fluorescent dyes, cross-linking reagents, neutron-diffraction or antibodies against ribosomal proteins seem to be most successful approaches. Structure and function of ribosomes can be correlated be depleting the complete ribosomes of some proteins to the functionally inactive core and by subsequent partial reconstitution in order to regain active ribosomal particles.

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