scholarly journals Mid-Infrared Microscopy via Position Correlations of Undetected Photons

2021 ◽  
Author(s):  
Inna Kviatkovsky ◽  
Helen Chrzanowski ◽  
Sven Ramelow
Keyword(s):  
Infrared Microscopy ◽  
Mid Infrared

Laser speckle reduction techniques for mid-infrared microscopy and stand-off spectroscopy

2017 ◽  
Author(s):  
Robert Furstenberg ◽  
Christopher A. Kendziora ◽  
Christopher J. Breshike ◽  
Viet Nguyen ◽  
R. Andrew McGill
Keyword(s):  
Laser Speckle ◽  
Speckle Reduction ◽  
Infrared Microscopy ◽  
Mid Infrared ◽  
Reduction Techniques

scholarly journals Doubling the far-field resolution in mid-infrared microscopy

2016 ◽  
Vol 24 (21) ◽  
pp. 24377 ◽  
Author(s):  
Mahendar Kumbham ◽  
Susan Daly ◽  
Kevin O’Dwyer ◽  
Rabah Mouras ◽  
Ning Liu ◽  
...  
Keyword(s):  
Far Field ◽  
Infrared Microscopy ◽  
Mid Infrared

scholarly journals Mid-infrared optoacoustic microscopy with label-free chemical contrast in living cells and tissues

2018 ◽  
Author(s):  
Miguel A. Pleitez ◽  
Asrar Ali Khan ◽  
Josefine Reber ◽  
Andriy Chmyrov ◽  
Markus R. Seeger ◽  
...  
Keyword(s):  
Imaging Modality ◽  
Living Cells ◽  
Spatiotemporal Dynamics ◽  
Live Cell ◽  
Imaging Modalities ◽  
Label Free ◽  
Infrared Microscopy ◽  
Mid Infrared ◽  
Infrared Sensing ◽  
Biomolecular Imaging

We developed mid-infrared optoacoustic microscopy (MiROM), a bond-selective imaging modality that overcomes water/tissue opacity and depth limitations of mid-infrared sensing allowing uncompromised live-cell/thick-tissue mid-infrared microscopy with up to three orders of magnitudehigher sensitivity than other vibrational imaging modalities; such as Raman. We showcase the functional label-free biomolecular imaging capabilities of MiROM by monitoring the spatiotemporal dynamics of lipids and proteins during lipolysis in living adipocytes. Since MiROM, contrary to Ramanmodalities, is not only able to detect lipids and proteins, but also important metabolites such as glucose without the need of labels, here we discuss how MiROM yields novel functional label-free abilities for a broader range of analytical studies in living cells and tissues.

scholarly journals Toward Mid-Infrared, Subdiffraction, Spectral-Mapping of Human Cells and Tissue: SNIM (Scanning Near-Field Infrared Microscopy) Tip Fabrication

2016 ◽  
Vol 34 (4) ◽  
pp. 1212-1219 ◽  
Author(s):  
Giorgos S. Athanasiou ◽  
Johanna Ernst ◽  
David Furniss ◽  
Trevor M. Benson ◽  
Jasbinder Chauhan ◽  
...  
Keyword(s):  
Near Field ◽  
Human Cells ◽  
Infrared Microscopy ◽  
Spectral Mapping ◽  
Mid Infrared

Advancements in quantum cascade laser-based infrared microscopy of aqueous media

2016 ◽  
Vol 187 ◽  
pp. 119-134 ◽  
Author(s):  
K. Haase ◽  
N. Kröger-Lui ◽  
A. Pucci ◽  
A. Schönhals ◽  
W. Petrich
Keyword(s):  
Quantum Cascade Laser ◽  
Quantum Cascade Lasers ◽  
Spectral Power ◽  
Aqueous Media ◽  
Aqueous Environment ◽  
Label Free ◽  
Infrared Microscopy ◽  
Signal To Noise ◽  
Mid Infrared ◽  
Quantum Cascade

The large mid-infrared absorption coefficient of water frequently hampers the rapid, label-free infrared microscopy of biological objects in their natural aqueous environment. However, the high spectral power density of quantum cascade lasers is shifting this limitation such that mid-infrared absorbance images can be acquired in situ within signal-to-noise ratios of up to 100. Even at sample thicknesses well above 50 μm, signal-to-noise ratios above 10 are readily achieved. The quantum cascade laser-based microspectroscopy of aqueous media is exemplified by imaging an aqueous yeast solution and quantifying glucose consumption, ethanol generation as well as the production of carbon dioxide gas during fermentation.

A graphene-based tunable guided-mode resonance narrowband mid-infrared filter

2018 ◽  
Vol 32 (20) ◽  
pp. 1850227 ◽  
Author(s):  
Yan-Lin Liao ◽  
Yan Zhao ◽  
Wen Zhang ◽  
Sujuan Feng
Keyword(s):  
Fermi Level ◽  
Infrared Microscopy ◽  
Mid Infrared ◽  
The Real ◽  
Guided Mode ◽  
Reflection Peak ◽  
Guided Mode Resonance ◽  
Narrowband Filter

We report a graphene-based tunable ultra-narrowband mid-infrared filter which can be tuned from 4.45122 [Formula: see text]m to 4.44675 [Formula: see text]m by tuning the Fermi level from 0.2 eV to 0.6 eV. Furthermore, the reflection bandwidth is less than 0.2 nm and the reflection rate is more than 0.55. The ultra-narrowband filter is designed based on the guided-mode resonance (GMR) effect. The shift of reflection peak is mainly caused by the change of the real part of the graphene’s permittivity. This tunable ultra-narrowband mid-infrared filter can be applied in the mid-infrared microscopy.

High definition infrared chemical imaging of colorectal tissue using a Spero QCL microscope

The Analyst ◽  
2017 ◽  
Vol 142 (8) ◽  
pp. 1381-1386 ◽  
Author(s):  
B. Bird ◽  
J. Rowlette
Keyword(s):  
High Throughput ◽  
Chemical Imaging ◽  
Biomedical Science ◽  
Infrared Microscopy ◽  
High Definition ◽  
Mid Infrared ◽  
Colorectal Tissue ◽  
Infrared Microscope

Mid-infrared microscopy has become a key technique in the field of biomedical science and spectroscopy. In this current study, we explore the use of a QCL infrared microscope to produce high definition, high throughput chemical images useful for the screening of biopsied colorectal tissue.

scholarly journals Synchrotron-Infrared Microscopy Analysis of Amyloid Fibrils Irradiated by Mid-Infrared Free-Electron Laser

2014 ◽  
Vol 05 (06) ◽  
pp. 384-394 ◽  
Author(s):  
Takayasu Kawasaki ◽  
Toyonari Yaji ◽  
Takayuki Imai ◽  
Toshiaki Ohta ◽  
Koichi Tsukiyama
Keyword(s):  
Free Electron ◽  
Free Electron Laser ◽  
Amyloid Fibrils ◽  
Infrared Microscopy ◽  
Mid Infrared ◽  
Microscopy Analysis

A protocol for rapid, label-free histochemical imaging of fibrotic liver

The Analyst ◽  
2017 ◽  
Vol 142 (8) ◽  
pp. 1179-1184 ◽  
Author(s):  
B. Bird ◽  
J. Rowlette
Keyword(s):  
High Throughput ◽  
Imaging Technique ◽  
Spectroscopic Imaging ◽  
Label Free ◽  
Biochemical Screening ◽  
Infrared Microscopy ◽  
Fibrotic Liver ◽  
Mid Infrared ◽  
Non Destructive

Mid-infrared microscopy is a non-destructive, quantitative and label-free spectroscopic imaging technique that, as a result of recent instrument advancements, is now at the point of enabling high-throughput automated biochemical screening of whole histology samples.

scholarly journals Depth-resolved mid-infrared photothermal imaging of living cells and organisms with submicrometer spatial resolution

2016 ◽  
Vol 2 (9) ◽  
pp. e1600521 ◽  
Author(s):  
Delong Zhang ◽  
Chen Li ◽  
Chi Zhang ◽  
Mikhail N. Slipchenko ◽  
Gregory Eakins ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Single Cells ◽  
Chemical Imaging ◽  
Detection Sensitivity ◽  
Living Systems ◽  
Live Cells ◽  
Photothermal Effect ◽  
Label Free ◽  
Infrared Microscopy ◽  
Mid Infrared

Chemical contrast has long been sought for label-free visualization of biomolecules and materials in complex living systems. Although infrared spectroscopic imaging has come a long way in this direction, it is thus far only applicable to dried tissues because of the strong infrared absorption by water. It also suffers from low spatial resolution due to long wavelengths and lacks optical sectioning capabilities. We overcome these limitations through sensing vibrational absorption–induced photothermal effect by a visible laser beam. Our mid-infrared photothermal (MIP) approach reached 10 μM detection sensitivity and submicrometer lateral spatial resolution. This performance has exceeded the diffraction limit of infrared microscopy and allowed label-free three-dimensional chemical imaging of live cells and organisms. Distributions of endogenous lipid and exogenous drug inside single cells were visualized. We further demonstrated in vivo MIP imaging of lipids and proteins inCaenorhabditis elegans. The reported MIP imaging technology promises broad applications from monitoring metabolic activities to high-resolution mapping of drug molecules in living systems, which are beyond the reach of current infrared microscopy.

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