scholarly journals Enhancing axial resolution and background rejection in line-scanning temporal focusing microscopy by focal modulation

2018 ◽  
Vol 26 (17) ◽  
pp. 21518 ◽  
Author(s):  
Yuanlong Zhang ◽  
Lingjie Kong ◽  
Hao Xie ◽  
Xiaofei Han ◽  
Qionghai Dai
Keyword(s):  
Axial Resolution ◽  
Background Rejection ◽  
Temporal Focusing ◽  
Line Scanning

scholarly journals Photobleaching Imprinting Enhanced Background Rejection in Line-Scanning Temporal Focusing Microscopy

2020 ◽  
Vol 8 ◽  
Author(s):  
Chaowei Zhuang ◽  
Xinyang Li ◽  
Yuanlong Zhang ◽  
Lingjie Kong ◽  
Hao Xie ◽  
...  
Keyword(s):  
High Speed ◽  
Mammary Epithelial Cells ◽  
Focal Depth ◽  
Time Lapse ◽  
Decay Rates ◽  
High Speed Imaging ◽  
Background Rejection ◽  
Two Photon ◽  
Temporal Focusing ◽  
Line Scanning

Compared with two-photon point-scanning microscopy, two-photon temporal focusing microscopy (2pTFM) provides a parallel high-speed imaging strategy with optical sectioning capability. Owing to out-of-focus fluorescence induced by scattering, 2pTFM suffers deteriorated signal-to-background ratio (SBR) for deep imaging in turbid tissue, Here, we utilized the photobleaching property of fluorophore to eliminate out-of-focus fluorescence. According to different decay rates in different focal depth, we extract the in-focus signals out of backgrounds through time-lapse images. We analyzed the theoretical foundations of photobleaching imprinting of the line-scanning temporal focusing microscopy, simulated implementation for background rejection, and demonstrated the contrast enhancement in MCF-10A human mammary epithelial cells and cleared Thy1-YFP mouse brains. More than 50% of total background light rejection was achieved, providing higher SBR images of the MCF-10A samples and mouse brains. The photobleaching imprinting method can be easily adapted to other fluorescence dyes or proteins, which may have application in studies involving relatively large and nontransparent organisms.

scholarly journals HiLo Based Line Scanning Temporal Focusing Microscopy for High-Speed, Deep Tissue Imaging

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 634
Author(s):  
Ruheng Shi ◽  
Yuanlong Zhang ◽  
Tiankuang Zhou ◽  
Lingjie Kong
Keyword(s):  
High Speed ◽  
Three Dimensions ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
High Temporal Resolution ◽  
Structured Illumination ◽  
Volumetric Imaging ◽  
Temporal Focusing ◽  
Line Scanning

High-speed, optical-sectioning imaging is highly desired in biomedical studies, as most bio-structures and bio-dynamics are in three-dimensions. Compared to point-scanning techniques, line scanning temporal focusing microscopy (LSTFM) is a promising method that can achieve high temporal resolution while maintaining a deep penetration depth. However, the contrast and axial confinement would still be deteriorated in scattering tissue imaging. Here, we propose a HiLo-based LSTFM, utilizing structured illumination to inhibit the fluorescence background and, thus, enhance the image contrast and axial confinement in deep imaging. We demonstrate the superiority of our method by performing volumetric imaging of neurons and dynamical imaging of microglia in mouse brains in vivo.

scholarly journals Scattering reduction by structured light illumination in line-scanning temporal focusing microscopy

2018 ◽  
Vol 9 (11) ◽  
pp. 5654 ◽  
Author(s):  
Yi Xue ◽  
Kalen P. Berry ◽  
Josiah R. Boivin ◽  
Dushan Wadduwage ◽  
Elly Nedivi ◽  
...  
Keyword(s):  
Structured Light ◽  
Light Illumination ◽  
Structured Light Illumination ◽  
Temporal Focusing ◽  
Line Scanning

scholarly journals Fast two-snapshot structured illumination for temporal focusing microscopy with enhanced axial resolution

2017 ◽  
Vol 25 (19) ◽  
pp. 23109 ◽  
Author(s):  
Yunlong Meng ◽  
Wei Lin ◽  
Chenglin Li ◽  
Shih-chi Chen
Keyword(s):  
Structured Illumination ◽  
Axial Resolution ◽  
Temporal Focusing

Deep line-temporal focusing with high axial resolution and a large field-of-view using intracavity control and incoherent pulse shaping

2018 ◽  
Vol 43 (20) ◽  
pp. 4919 ◽  
Author(s):  
Kai Lou ◽  
Bo Wang ◽  
Ah-Young Jee ◽  
Steve Granick ◽  
François Amblard
Keyword(s):  
Pulse Shaping ◽  
Field Of View ◽  
Large Field ◽  
Axial Resolution ◽  
Temporal Focusing
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