Deep line-temporal focusing with high axial resolution and a large field-of-view using intracavity control and incoherent pulse shaping

2018 ◽  
Vol 43 (20) ◽  
pp. 4919 ◽  
Author(s):  
Kai Lou ◽  
Bo Wang ◽  
Ah-Young Jee ◽  
Steve Granick ◽  
François Amblard
Keyword(s):  
Pulse Shaping ◽  
Field Of View ◽  
Large Field ◽  
Axial Resolution ◽  
Temporal Focusing

scholarly journals Multiview tiling light sheet microscopy for 3D high resolution live imaging

Development ◽  
2021 ◽  
Author(s):  
Mostafa Aakhte ◽  
H.-Arno J. Müller
Keyword(s):  
High Resolution ◽  
Myosin Ii ◽  
Resolution Enhancement ◽  
Light Sheet ◽  
Field Of View ◽  
Large Field ◽  
Axial Resolution ◽  
Light Sheet Microscopy ◽  
Drosophila Embryogenesis ◽  
Mechanical Rotation

Light sheet or selective plane illumination microscopy (SPIM) is ideally suited for in toto imaging of living specimens at high temporal-spatial resolution. In SPIM, the light scattering that occurs during imaging of opaque specimens brings about limitations in terms of resolution and the imaging field of view. To ameliorate this shortcoming, the illumination beam can be engineered into a highly confined light sheet over a large field of view and multi-view imaging can be performed by applying multiple lenses combined with mechanical rotation of the sample. Here, we present a Multiview tiling SPIM (MT-SPIM) that combines the Multi-view SPIM (M-SPIM) with a confined, multi-tiled light sheet. The MT-SPIM provides high-resolution, robust and rotation-free imaging of living specimens. We applied the MT-SPIM to image nuclei and Myosin II from the cellular to subcellular spatial scale in early Drosophila embryogenesis. We show that the MT-SPIM improves the axial-resolution relative to the conventional M-SPIM by a factor of two. We further demonstrate that this axial resolution enhancement improves the automated segmentation of Myosin II distribution and of nuclear volumes and shapes.

scholarly journals Multiview tiling light sheet microscopy for 3D high resolution live imaging

2021 ◽  
Author(s):  
Mostafa Aakhte ◽  
Hans-Arno J Mueller
Keyword(s):  
High Resolution ◽  
Myosin Ii ◽  
Resolution Enhancement ◽  
Light Sheet ◽  
Field Of View ◽  
Large Field ◽  
Axial Resolution ◽  
Light Sheet Microscopy ◽  
Drosophila Embryogenesis ◽  
Mechanical Rotation

Light sheet or selective plane illumination microscopy (SPIM) is ideally suited for in toto imaging of living specimens at high temporal-spatial resolution. In SPIM, the light scattering that occurs during imaging of opaque specimens brings about limitations in terms of resolution and the imaging field of view. To ameliorate this shortcoming, the illumination beam can be engineered into a highly confined light sheet over a large field of view and multi-view imaging can be performed by applying multiple lenses combined with mechanical rotation of the sample. Here, we present a Multiview tiling SPIM (MT-SPIM) that combines the Multi-view SPIM (M-SPIM) with a confined, multi-tiled light sheet. The MT-SPIM provides high-resolution, robust and rotation-free imaging of living specimens. We applied the MT-SPIM to image nuclei and Myosin II from the cellular to subcellular spatial scale in early Drosophila embryogenesis. We show that the MT-SPIM improves the axial-resolution relative to the conventional M-SPIM by a factor of two. We further demonstrate that this axial resolution enhancement improves the automated segmentation of Myosin II distribution and of nuclear volumes and shapes.

scholarly journals Light-sheet microscopy with isotropic, sub-micron resolution and solvent-independent large-scale imaging

2019 ◽  
Author(s):  
Tonmoy Chakraborty ◽  
Meghan Driscoll ◽  
Malea Murphy ◽  
Philippe Roudot ◽  
Bo-Jui Chang ◽  
...  
Keyword(s):  
Dendritic Spines ◽  
Large Scale ◽  
Light Sheet ◽  
Automated Detection ◽  
Field Of View ◽  
Large Field ◽  
Axial Resolution ◽  
Optical Sectioning ◽  
Isotropic Resolution ◽  
Light Sheet Microscopy

AbstractWe present cleared tissue Axially Swept Light-Sheet Microscopy (ctASLM), which achieves sub-micron isotropic resolution, high optical sectioning capability, and large field of view imaging (870×870 μm2) over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and organic chemically cleared tissue preparations and provides 2- to 5-fold better axial resolution than confocal or other reported cleared tissue light-sheet microscopes. We image millimeter-sized tissues with sub-micron 3D resolution, which enabled us to perform automated detection of cells and subcellular features such as dendritic spines.

Numerical analysis of shift error in X-ray phase contrast imaging for large field of view

2017 ◽  
Vol 34 (1) ◽  
pp. 8
Author(s):  
Jianheng Huang ◽  
Yaohu Lei ◽  
Xin Liu ◽  
Jinchuan Guo ◽  
Ji Li ◽  
...  
Keyword(s):  
Numerical Analysis ◽  
Phase Contrast ◽  
Field Of View ◽  
Phase Contrast Imaging ◽  
Large Field ◽  
Contrast Imaging ◽  
X Ray

Large Field-of-View Super-Resolution Optical Microscopy Based on Planar Polymer Waveguides

ACS Photonics ◽  
2021 ◽  
Author(s):  
Anders Kokkvoll Engdahl ◽  
Stefan Belle ◽  
Tung-Cheng Wang ◽  
Ralf Hellmann ◽  
Thomas Huser ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Super Resolution ◽  
Field Of View ◽  
Large Field ◽  
Polymer Waveguides

scholarly journals Attitude determination for nano-satellites – I. Spherical projections for large field of view infrasensors

2021 ◽  
Author(s):  
Kornél Kapás ◽  
Tamás Bozóki ◽  
Gergely Dálya ◽  
János Takátsy ◽  
László Mészáros ◽  
...  
Keyword(s):  
Attitude Determination ◽  
Field Of View ◽  
Large Field

A novel and accurate calibration method for cameras with large field of view using combined small targets

Measurement ◽  
2015 ◽  
Vol 64 ◽  
pp. 1-16 ◽  
Author(s):  
Zhen Liu ◽  
Fengjiao Li ◽  
Xiaojing Li ◽  
Guangjun Zhang
Keyword(s):  
Calibration Method ◽  
Field Of View ◽  
Large Field ◽  
Small Targets
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