scholarly journals Fast two-snapshot structured illumination for temporal focusing microscopy with enhanced axial resolution

2017 ◽  
Vol 25 (19) ◽  
pp. 23109 ◽  
Author(s):  
Yunlong Meng ◽  
Wei Lin ◽  
Chenglin Li ◽  
Shih-chi Chen
Keyword(s):  
Structured Illumination ◽  
Axial Resolution ◽  
Temporal Focusing

Multiphoton 3D structured illumination microscopy for enhanced axial resolution in deep imaging

2015 ◽  
Author(s):  
Qiyuan Song ◽  
Keisuke Isobe ◽  
Fumihiko Kannari ◽  
Hiroyuki Kawano ◽  
Akiko Kumagai ◽  
...  
Keyword(s):  
Structured Illumination ◽  
Axial Resolution ◽  
Structured Illumination Microscopy ◽  
Deep Imaging

scholarly journals Temporal focusing microscopy combined with three-dimensional structured illumination

2017 ◽  
Vol 56 (5) ◽  
pp. 052501 ◽  
Author(s):  
Keisuke Isobe ◽  
Keisuke Toda ◽  
Qiyuan Song ◽  
Fumihiko Kannari ◽  
Hiroyuki Kawano ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Structured Illumination ◽  
Temporal Focusing

scholarly journals Full three-dimensional imaging deep through multicellular thick samples with subcellular resolution by structured illumination microscopy and adaptive optics

2020 ◽  
Author(s):  
Ruizhe Lin ◽  
Edward T. Kipreos ◽  
Jie Zhu ◽  
Chang Hyun Khang ◽  
Peter Kner
Keyword(s):  
Adaptive Optics ◽  
Three Dimensional ◽  
Structured Illumination ◽  
Axial Resolution ◽  
Structured Illumination Microscopy ◽  
Optical Aberrations ◽  
C Elegans ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Subcellular Resolution

AbstractStructured Illumination Microscopy enables live imaging with resolutions of ~120 nm. Unfortunately, optical aberrations can lead to loss of resolution and artifacts in Structured Illumination Microscopy rendering the technique unusable in samples thicker than a single cell. Here we report on the combination of Adaptive Optics and Structured Illumination Microscopy enabling imaging with 140 nm lateral and 585 nm axial resolution in tissue culture cells, C. elegans, and rice blast fungus. We demonstrate that AO improves resolution and reduces artifacts, making full 3D SIM possible in thicker samples.

Temporal focusing microscopy with structured illumination for super-resolution deep imaging

CLEO: 2014 ◽  
2014 ◽  
Author(s):  
Keisuke Isobe ◽  
Kyohei Mochizuki ◽  
Qiyuan Song ◽  
Akira Suda ◽  
Fumihiko Kannari ◽  
...  
Keyword(s):  
Super Resolution ◽  
Structured Illumination ◽  
Temporal Focusing ◽  
Deep Imaging

Generation and Control of Wide-Field Three-Dimensional Structured Illumination for Advanced Microscopic Imaging

2012 ◽  
Vol 516 ◽  
pp. 640-644
Author(s):  
Shin Usuki ◽  
Hiroyoshi Kanaka ◽  
Kenjiro Takai Miura
Keyword(s):  
Three Dimensional ◽  
Fluorescent Imaging ◽  
Wide Field ◽  
Structured Illumination ◽  
Axial Resolution ◽  
Structured Illumination Microscopy ◽  
Spatial Control ◽  
Microscopic Imaging ◽  
Point Spread ◽  
Resolution Improvement

In a variety of practical microscopic imaging applications, many industries require not only lateral resolution improvement but also axial resolution improvement. The resolution in optical microscopy is limited by diffraction and determined by the wavelength of the incident light and the numerical aperture (NA) of the objective lens. The diffraction limit is mathematically described by a point spread function in the imaging system, and three-dimensional (3D) point spread functions describe both the lateral and axial resolutions. Thus, it is useful to focus on exceeding this limit and improving the resolution of optical imaging by the spatial control of structured illumination. Structured illumination microscopy is a familiar technique to improve resolution in fluorescent imaging, and it is expected to be applied to industrial applications. Microscopic imaging is convenient, non-destructive, and has a high-throughput performance and compatibility with a number of applications. However, the spatial resolution of conventional light microscopy is limited to wavelength scale and the depth of field is shallow; hence, it is difficult to obtain detailed 3D spatial data of the object to be measured. Here, we propose a new technique for generating and controlling wide-field 3D structured illumination. The technique, based on the 3D interference of multiple laser beams, provides lateral and axial resolution improvement, and a wide 3D field of view. The spatial configuration of the beams was theoretically examined and the optimal incident angle of the multiple beams was confirmed. Numerical simulations using the finite difference time domain (FDTD) method were carried out and confirmed the generation of 3D structured illumination and spatial control of the illumination by using the phase shift of incident beams.

scholarly journals Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device

2014 ◽  
Vol 5 (8) ◽  
pp. 2526 ◽  
Author(s):  
Li-Chung Cheng ◽  
Chi-Hsiang Lien ◽  
Yong Da Sie ◽  
Yvonne Yuling Hu ◽  
Chun-Yu Lin ◽  
...  
Keyword(s):  
Digital Micromirror Device ◽  
Structured Illumination ◽  
Multiphoton Excitation ◽  
Temporal Focusing

scholarly journals Improving axial resolution in Structured Illumination Microscopy using deep learning

2021 ◽  
Vol 379 (2199) ◽  
Author(s):  
Miguel A. Boland ◽  
Edward A. K. Cohen ◽  
Seth R. Flaxman ◽  
Mark A. A. Neil
Keyword(s):  
Deep Learning ◽  
Optical Microscopy ◽  
Super Resolution ◽  
Structured Illumination ◽  
Learning Models ◽  
Axial Resolution ◽  
Structured Illumination Microscopy ◽  
Recent Advances ◽  
Robust To Noise ◽  
Up Scaling

Structured Illumination Microscopy (SIM) is a widespread methodology to image live and fixed biological structures smaller than the diffraction limits of conventional optical microscopy. Using recent advances in image up-scaling through deep learning models, we demonstrate a method to reconstruct 3D SIM image stacks with twice the axial resolution attainable through conventional SIM reconstructions. We further demonstrate our method is robust to noise and evaluate it against two-point cases and axial gratings. Finally, we discuss potential adaptions of the method to further improve resolution. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)’.

scholarly journals HiLo Based Line Scanning Temporal Focusing Microscopy for High-Speed, Deep Tissue Imaging

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 634
Author(s):  
Ruheng Shi ◽  
Yuanlong Zhang ◽  
Tiankuang Zhou ◽  
Lingjie Kong
Keyword(s):  
High Speed ◽  
Three Dimensions ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
High Temporal Resolution ◽  
Structured Illumination ◽  
Volumetric Imaging ◽  
Temporal Focusing ◽  
Line Scanning

High-speed, optical-sectioning imaging is highly desired in biomedical studies, as most bio-structures and bio-dynamics are in three-dimensions. Compared to point-scanning techniques, line scanning temporal focusing microscopy (LSTFM) is a promising method that can achieve high temporal resolution while maintaining a deep penetration depth. However, the contrast and axial confinement would still be deteriorated in scattering tissue imaging. Here, we propose a HiLo-based LSTFM, utilizing structured illumination to inhibit the fluorescence background and, thus, enhance the image contrast and axial confinement in deep imaging. We demonstrate the superiority of our method by performing volumetric imaging of neurons and dynamical imaging of microglia in mouse brains in vivo.

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