scholarly journals Three-dimensional combined photoacoustic and optical coherence microscopy for in vivo microcirculation studies

2009 ◽  
Vol 17 (19) ◽  
pp. 16450 ◽  
Author(s):  
Li Li ◽  
Konstantin Maslov ◽  
Geng Ku ◽  
Lihong V. Wang
Keyword(s):  
Three Dimensional ◽  
Optical Coherence ◽  
Optical Coherence Microscopy

In vivo three-dimensional imaging of plants with optical coherence microscopy

2002 ◽  
Vol 208 (3) ◽  
pp. 177-189 ◽  
Author(s):  
A. Reeves ◽  
R. L. Parsons ◽  
J. W. Hettinger ◽  
J. I. Medford
Keyword(s):  
Three Dimensional ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Three Dimensional Imaging

scholarly journals In Vivo Rat Brain Imaging through Full-Field Optical Coherence Microscopy Using an Ultrathin Short Multimode Fiber Probe

2019 ◽  
Vol 9 (2) ◽  
pp. 216 ◽  
Author(s):  
Manabu Sato ◽  
Kai Eto ◽  
Junpei Masuta ◽  
Kenji Inoue ◽  
Reiko Kurotani ◽  
...  
Keyword(s):  
Rat Brain ◽  
Three Dimensional ◽  
Nerve Fibers ◽  
Outer Diameter ◽  
Optical Coherence ◽  
Multimode Fiber ◽  
Core Diameter ◽  
Optical Coherence Microscopy ◽  
Full Field

We demonstrate full-field optical coherence microscopy (OCM) using an ultrathin forward-imaging short multimode fiber (SMMF) probe with a core diameter of 50 μm, outer diameter of 125 μm, and length of 7.4 mm, which is a typical graded-index multimode fiber used for optical communications. The axial and lateral resolutions were measured to be 2.14 μm and 2.3 μm, respectively. By inserting the SMMF 4 mm into the cortex of an in vivo rat brain, scanning was performed to a depth of 147 μm from the SMMF facet with a field of view of 47 μm. Three-dimensional (3D) OCM images were obtained at depths ranging from approximately 20 μm to 90 μm. Based on the morphological information of the resliced 3D images and the dependence of the integration of the OCM image signal on the insertion length, the obtained 3D information of nerve fibers has been presented.

scholarly journals 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Jun Zhu ◽  
Hercules Rezende Freitas ◽  
Izumi Maezawa ◽  
Lee-way Jin ◽  
Vivek J. Srinivasan
Keyword(s):  
Minimally Invasive ◽  
Mouse Brain ◽  
Numerical Aperture ◽  
Optical Biopsy ◽  
Optical Coherence ◽  
Label Free ◽  
Optical Coherence Microscopy ◽  
Minimal Invasiveness ◽  
Cortical Regions

AbstractIn vivo, minimally invasive microscopy in deep cortical and sub-cortical regions of the mouse brain has been challenging. To address this challenge, we present an in vivo high numerical aperture optical coherence microscopy (OCM) approach that fully utilizes the water absorption window around 1700 nm, where ballistic attenuation in the brain is minimized. Key issues, including detector noise, excess light source noise, chromatic dispersion, and the resolution-speckle tradeoff, are analyzed and optimized. Imaging through a thinned-skull preparation that preserves intracranial space, we present volumetric imaging of cytoarchitecture and myeloarchitecture across the entire depth of the mouse neocortex, and some sub-cortical regions. In an Alzheimer’s disease model, we report that findings in superficial and deep cortical layers diverge, highlighting the importance of deep optical biopsy. Compared to other microscopic techniques, our 1700 nm OCM approach achieves a unique combination of intrinsic contrast, minimal invasiveness, and high resolution for deep brain imaging.

scholarly journals In vivo imaging of corneal nerves and cellular structures in mice with Gabor-domain optical coherence microscopy

2020 ◽  
Vol 11 (2) ◽  
pp. 711 ◽  
Author(s):  
Cristina Canavesi ◽  
Andrea Cogliati ◽  
Amanda Mietus ◽  
Yue Qi ◽  
Jesse Schallek ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Cellular Structures ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Corneal Nerves ◽  
Gabor Domain
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