scholarly journals In vivo measurement of erythrocyte velocity and retinal blood flow using adaptive optics scanning laser ophthalmoscopy

2008 ◽  
Vol 16 (17) ◽  
pp. 12746 ◽  
Author(s):  
Zhangyi Zhong ◽  
Benno L. Petrig ◽  
Xiaofeng Qi ◽  
Stephen A. Burns
Keyword(s):  
Blood Flow ◽  
Adaptive Optics ◽  
Retinal Blood Flow ◽  
Scanning Laser ◽  
Scanning Laser Ophthalmoscopy ◽  
In Vivo Measurement

scholarly journals Retinal Blood Velocity and Flow in Early Diabetes and Diabetic Retinopathy Using Adaptive Optics Scanning Laser Ophthalmoscopy

2019 ◽  
Vol 8 (8) ◽  
pp. 1165 ◽  
Author(s):  
Cherilyn Mae A. Palochak ◽  
Hee Eun Lee ◽  
Jessica Song ◽  
Andrew Geng ◽  
Robert A. Linsenmeier ◽  
...  
Keyword(s):  
Blood Flow ◽  
Diabetic Retinopathy ◽  
Adaptive Optics ◽  
Blood Velocity ◽  
Vessel Density ◽  
Retinal Blood Flow ◽  
Low Flow ◽  
Scanning Laser ◽  
Diabetic Patients ◽  
Scanning Laser Ophthalmoscopy

Using adaptive optics scanning laser ophthalmoscopy (AOSLO), we measured retinal blood velocity and flow in healthy control eyes and eyes of diabetic patients with or without retinopathy. This cross-sectional study included 39 eyes of 30 patients with diabetes (DM) with mild non-proliferative diabetic retinopathy (NPDR) or without retinopathy (DM no DR) and 21 eyes of 17 healthy age-matched controls. Participants were imaged with a commercial optical coherence tomography angiography (OCTA) device (RTVue-XR Avanti) and AOSLO device (Apaeros Retinal Imaging System, Boston Micromachines). We analyzed AOSLO-based retinal blood velocity and flow, and OCTA-based vessel density of the superficial (SCP), deep retinal capillary plexus (DCP), and full retina. Retinal blood velocity was significantly higher in eyes with DM no DR and lower in NPDR across all vessel diameters compared to controls. Retinal blood flow was significantly higher in DM no DR and lower in NPDR in vessel diameters up to 60 μm compared to controls. When comparing flow outliers (low-flow DM no DR eyes and high-flow NPDR eyes), we found they had a significantly different retinal vessel density compared to the remaining eyes in the respective groups. Retinal blood velocity and flow is increased in eyes with DM no DR, while these parameters are decreased in eyes with mild NPDR compared to healthy age-matched controls. The similarity of OCTA vessel density among outliers in the two diabetic groups suggests an initial increase followed by progressive decline in blood flow and OCTA vessel density with progression to clinical retinopathy, which warrants further investigation.

scholarly journals Adaptive optics scanning laser ophthalmoscopy for in vivo imaging of lamina cribrosa

2007 ◽  
Vol 24 (5) ◽  
pp. 1417 ◽  
Author(s):  
Abhiram S. Vilupuru ◽  
Nalini V. Rangaswamy ◽  
Laura J. Frishman ◽  
Earl L. Smith III ◽  
Ronald S. Harwerth ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
In Vivo Imaging ◽  
Lamina Cribrosa ◽  
Scanning Laser ◽  
Scanning Laser Ophthalmoscopy

scholarly journals Characterization of In Vivo Retinal Lesions of Diabetic Retinopathy Using Adaptive Optics Scanning Laser Ophthalmoscopy

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Sonja G. Karst ◽  
Jan Lammer ◽  
Salma H. Radwan ◽  
Hanna Kwak ◽  
Paolo S. Silva ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Adaptive Optics ◽  
Scattered Light ◽  
Disease Onset ◽  
Morphological Characteristics ◽  
Response To Therapy ◽  
Vascular Lesions ◽  
Scanning Laser ◽  
Scanning Laser Ophthalmoscopy

Purpose. To characterize hallmark diabetic retinopathy (DR) lesions utilizing adaptive optics scanning laser ophthalmoscopy (AOSLO) and to compare AOSLO findings with those on standard imaging techniques. Methods. Cross-sectional study including 35 eyes of 34 study participants. AOSLO confocal and multiply scattered light (MSL) imaging were performed in eyes with DR. Color fundus photographs (CF), infrared images of the macula (Spectralis, Heidelberg), and Spectralis spectral domain optical coherence tomography SDOCT B-scans of each lesion were obtained and registered to corresponding AOSLO images. Main Outcome Measures. Individual lesion characterization by AOSLO imaging. AOSLO appearance was compared with CF and SDOCT imaging. Results. Characterized lesions encompassed 52 microaneurysms (MA), 20 intraretinal microvascular abnormalities (IRMA), 7 neovascularization (NV), 11 hard exudates (HE), 5 dot/blot hemorrhages (HEM), 4 cotton wool spots (CWS), and 14 intraretinal cysts. AOSLO allowed assessment of perfusion in vascular lesions and enabled the identification of vascular lesions that could not be visualized on CF or SDOCT. Conclusions. AOSLO imaging provides detailed, noninvasive in vivo visualization of DR lesions enhancing the assessment of morphological characteristics. These unique AOSLO attributes may enable new insights into the pathological changes of DR in response to disease onset, development, regression, and response to therapy.

In Vivo Imaging of Lamina Cribrosa Pores by Adaptive Optics Scanning Laser Ophthalmoscopy

2012 ◽  
Vol 53 (7) ◽  
pp. 4111 ◽  
Author(s):  
Tadamichi Akagi ◽  
Masanori Hangai ◽  
Kohei Takayama ◽  
Atsushi Nonaka ◽  
Sotaro Ooto ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
In Vivo Imaging ◽  
Lamina Cribrosa ◽  
Scanning Laser ◽  
Scanning Laser Ophthalmoscopy

scholarly journals Large field-of-view incoherent volumetric imaging in living human retina by confocal oblique scanning laser ophthalmoscopy

2021 ◽  
Author(s):  
Wenjun Shao ◽  
Ji Yi
Keyword(s):  
Clinical Care ◽  
Retinal Imaging ◽  
Field Of View ◽  
Scanning Laser ◽  
Large Field ◽  
Scanning Laser Ophthalmoscopy ◽  
Human Retina ◽  
Label Free ◽  
Volumetric Imaging

Three-dimensional (3D) volumetric imaging of the human retina is instrumental to monitor and diagnose blinding conditions. Although coherent retinal imaging is well established by optical coherence tomography, it is still a large void for incoherent volumetric imaging in the human retina. Here, we report confocal oblique scanning laser ophthalmoscopy (CoSLO), to fill that void and harness incoherent optical contrast in 3D. CoSLO uses oblique scanning laser and remote focusing to acquire depth signal in parallel, avoid the lengthy z-stacking, and image a large field of view (FOV). In addition, confocal gating is introduced by a linear sensor array to improve the contrast and resolution. For the first time, we achieved incoherent 3D human retinal imaging with >20° viewing angle within only 5 seconds. The depth resolution is ~45 microns in vivo. We demonstrated label-free incoherent contrast by CoSLO, revealing unique features in the retina. CoSLO will be an important technique for clinical care of retinal conditions and fundamental vision science, by offering unique volumetric incoherent contrasts.

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