Fluorescent scanning laser ophthalmoscopy for cellular resolution in vivo mouse retinal imaging: benefits and drawbacks of implementing adaptive optics

2016 ◽  
Author(s):  
Pengfei Zhang ◽  
Mayank Goswami ◽  
Edward N. Pugh ◽  
Robert J. Zawadzki
Keyword(s):  
Adaptive Optics ◽  
Retinal Imaging ◽  
Scanning Laser ◽  
Scanning Laser Ophthalmoscopy ◽  
Cellular Resolution

scholarly journals Large field-of-view incoherent volumetric imaging in living human retina by confocal oblique scanning laser ophthalmoscopy

2021 ◽  
Author(s):  
Wenjun Shao ◽  
Ji Yi
Keyword(s):  
Clinical Care ◽  
Retinal Imaging ◽  
Field Of View ◽  
Scanning Laser ◽  
Large Field ◽  
Scanning Laser Ophthalmoscopy ◽  
Human Retina ◽  
Label Free ◽  
Volumetric Imaging

Three-dimensional (3D) volumetric imaging of the human retina is instrumental to monitor and diagnose blinding conditions. Although coherent retinal imaging is well established by optical coherence tomography, it is still a large void for incoherent volumetric imaging in the human retina. Here, we report confocal oblique scanning laser ophthalmoscopy (CoSLO), to fill that void and harness incoherent optical contrast in 3D. CoSLO uses oblique scanning laser and remote focusing to acquire depth signal in parallel, avoid the lengthy z-stacking, and image a large field of view (FOV). In addition, confocal gating is introduced by a linear sensor array to improve the contrast and resolution. For the first time, we achieved incoherent 3D human retinal imaging with >20° viewing angle within only 5 seconds. The depth resolution is ~45 microns in vivo. We demonstrated label-free incoherent contrast by CoSLO, revealing unique features in the retina. CoSLO will be an important technique for clinical care of retinal conditions and fundamental vision science, by offering unique volumetric incoherent contrasts.

scholarly journals Adaptive optics scanning laser ophthalmoscopy for in vivo imaging of lamina cribrosa

2007 ◽  
Vol 24 (5) ◽  
pp. 1417 ◽  
Author(s):  
Abhiram S. Vilupuru ◽  
Nalini V. Rangaswamy ◽  
Laura J. Frishman ◽  
Earl L. Smith III ◽  
Ronald S. Harwerth ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
In Vivo Imaging ◽  
Lamina Cribrosa ◽  
Scanning Laser ◽  
Scanning Laser Ophthalmoscopy

Clinical Applications of Retinal Imaging with Adaptive Optics

2011 ◽  
Vol 04 (02) ◽  
pp. 78 ◽  
Author(s):  
Joseph Carroll ◽  
Adam M Dubis ◽  
Pooja Godara ◽  
Alfredo Dubra ◽  
Kimberly E Stepien ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Retinal Imaging ◽  
Clinical Applications ◽  
Human Retina ◽  
Non Invasive ◽  
Cellular Resolution

The first images of the living human retina were published in 1886. In the 125 years since then, clinicians and researchers alike have relied on the continued development of new and improved ways to visualize the fundus to provide better in vivo views of the normal and diseased retina. Adaptive optics-based technologies offer non-invasive, cellular resolution views of the living retina, and bring with them several exciting clinical applications. Here we review some of these applications, with an emphasis on photoreceptor imaging.

scholarly journals Cellular resolution volumetric in vivo retinal imaging with adaptive optics–optical coherence tomography

2009 ◽  
Vol 17 (5) ◽  
pp. 4084 ◽  
Author(s):  
Robert J. Zawadzki ◽  
Stacey S. Choi ◽  
Alfred R. Fuller ◽  
Julia W. Evans ◽  
Bernd Hamann ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Adaptive Optics ◽  
Retinal Imaging ◽  
Optical Coherence ◽  
Cellular Resolution
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