Artifacts in computational optical-sectioning microscopy

1994 ◽  
Vol 11 (3) ◽  
pp. 1056 ◽  
Author(s):  
James G. McNally ◽  
Chrysanthe Preza ◽  
José-Angel Conchello ◽  
Lewis J. Thomas
Keyword(s):  
Optical Sectioning ◽  
Computational Optical Sectioning Microscopy

Point-spread sensitivity analysis for computational optical-sectioning microscopy

1992 ◽  
Vol 23 (4) ◽  
pp. 501-513 ◽  
Author(s):  
Chrysanthe Preza ◽  
John M. Ollinger ◽  
James G. McNally ◽  
Lewis J. Thomas
Keyword(s):  
Sensitivity Analysis ◽  
Optical Sectioning ◽  
Point Spread ◽  
Computational Optical Sectioning Microscopy

scholarly journals Challenges in 3D Live Cell Imaging

Photonics ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 275
Author(s):  
Herbert Schneckenburger ◽  
Verena Richter
Keyword(s):  
Live Cell Imaging ◽  
Laser Scanning ◽  
Cell Imaging ◽  
Live Cell ◽  
Laser Scanning Microscopy ◽  
Radiative Energy ◽  
Wide Field ◽  
Optical Sectioning ◽  
Continuous Increase ◽  
Radiative Energy Transfer

A short overview on 3D live cell imaging is given. Relevant samples are described and various problems and challenges—including 3D imaging by optical sectioning, light scattering and phototoxicity—are addressed. Furthermore, enhanced methods of wide-field or laser scanning microscopy together with some relevant examples and applications are summarized. In the future one may profit from a continuous increase in microscopic resolution, but also from molecular sensing techniques in the nanometer range using e.g., non-radiative energy transfer (FRET).

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