Computational optical-sectioning microscopy for 3D quantization of cell motion: results and challenges

1994 ◽  
Author(s):  
James G. McNally
Keyword(s):  
Optical Sectioning ◽  
Computational Optical Sectioning Microscopy ◽  
Cell Motion

Point-spread sensitivity analysis for computational optical-sectioning microscopy

1992 ◽  
Vol 23 (4) ◽  
pp. 501-513 ◽  
Author(s):  
Chrysanthe Preza ◽  
John M. Ollinger ◽  
James G. McNally ◽  
Lewis J. Thomas
Keyword(s):  
Sensitivity Analysis ◽  
Optical Sectioning ◽  
Point Spread ◽  
Computational Optical Sectioning Microscopy

Artifacts in computational optical-sectioning microscopy

1994 ◽  
Vol 11 (3) ◽  
pp. 1056 ◽  
Author(s):  
James G. McNally ◽  
Chrysanthe Preza ◽  
José-Angel Conchello ◽  
Lewis J. Thomas
Keyword(s):  
Optical Sectioning ◽  
Computational Optical Sectioning Microscopy

scholarly journals Chemotactic Responses of Jurkat Cells in Microfluidic Flow-Free Gradient Chambers

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 384 ◽  
Author(s):  
Utku M. Sonmez ◽  
Adam Wood ◽  
Kyle Justus ◽  
Weijian Jiang ◽  
Fatima Syed-Picard ◽  
...  
Keyword(s):  
Immune Response ◽  
Cancer Progression ◽  
Soft Lithography ◽  
Cell Movement ◽  
Population Level ◽  
Jurkat Cells ◽  
Dependent Manner ◽  
Diverse Range ◽  
Microfluidic Flow ◽  
Cell Motion

Gradients of soluble molecules coordinate cellular communication in a diverse range of multicellular systems. Chemokine-driven chemotaxis is a key orchestrator of cell movement during organ development, immune response and cancer progression. Chemotaxis assays capable of examining cell responses to different chemokines in the context of various extracellular matrices will be crucial to characterize directed cell motion in conditions which mimic whole tissue conditions. Here, a microfluidic device which can generate different chemokine patterns in flow-free gradient chambers while controlling surface extracellular matrix (ECM) to study chemotaxis either at the population level or at the single cell level with high resolution imaging is presented. The device is produced by combining additive manufacturing (AM) and soft lithography. Generation of concentration gradients in the device were simulated and experimentally validated. Then, stable gradients were applied to modulate chemotaxis and chemokinetic response of Jurkat cells as a model for T lymphocyte motility. Live imaging of the gradient chambers allowed to track and quantify Jurkat cell migration patterns. Using this system, it has been found that the strength of the chemotactic response of Jurkat cells to CXCL12 gradient was reduced by increasing surface fibronectin in a dose-dependent manner. The chemotaxis of the Jurkat cells was also found to be governed not only by the CXCL12 gradient but also by the average CXCL12 concentration. Distinct migratory behaviors in response to chemokine gradients in different contexts may be physiologically relevant for shaping the host immune response and may serve to optimize the targeting and accumulation of immune cells to the inflammation site. Our approach demonstrates the feasibility of using a flow-free gradient chamber for evaluating cross-regulation of cell motility by multiple factors in different biologic processes.

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