Super-resolution and point spread function sensitivity analysis of the expectation-maximization algorithm for computational optical sectioning microscopy

1994 ◽  
Author(s):  
Jose-Angel Conchello
Keyword(s):  
Sensitivity Analysis ◽  
Point Spread Function ◽  
Expectation Maximization ◽  
Expectation Maximization Algorithm ◽  
Super Resolution ◽  
Optical Sectioning ◽  
Point Spread ◽  
Spread Function ◽  
Computational Optical Sectioning Microscopy

scholarly journals High-fidelity structured illumination microscopy by point-spread-function engineering

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Gang Wen ◽  
Simin Li ◽  
Linbo Wang ◽  
Xiaohu Chen ◽  
Zhenglong Sun ◽  
...  
Keyword(s):  
Point Spread Function ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
High Fidelity ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Imaging Tool ◽  
Point Spread ◽  
Spread Function ◽  
Point Spread Function Engineering

AbstractStructured illumination microscopy (SIM) has become a widely used tool for insight into biomedical challenges due to its rapid, long-term, and super-resolution (SR) imaging. However, artifacts that often appear in SIM images have long brought into question its fidelity, and might cause misinterpretation of biological structures. We present HiFi-SIM, a high-fidelity SIM reconstruction algorithm, by engineering the effective point spread function (PSF) into an ideal form. HiFi-SIM can effectively reduce commonly seen artifacts without loss of fine structures and improve the axial sectioning for samples with strong background. In particular, HiFi-SIM is not sensitive to the commonly used PSF and reconstruction parameters; hence, it lowers the requirements for dedicated PSF calibration and complicated parameter adjustment, thus promoting SIM as a daily imaging tool.

scholarly journals Three-Dimensional Super-Resolution in Eukaryotic Cells Using the Double-Helix Point Spread Function

2017 ◽  
Vol 112 (7) ◽  
pp. 1444-1454 ◽  
Author(s):  
Alexander R. Carr ◽  
Aleks Ponjavic ◽  
Srinjan Basu ◽  
James McColl ◽  
Ana Mafalda Santos ◽  
...  
Keyword(s):  
Point Spread Function ◽  
Double Helix ◽  
Three Dimensional ◽  
Super Resolution ◽  
Eukaryotic Cells ◽  
Point Spread ◽  
Spread Function

scholarly journals Euclid: Nonparametric point spread function field recovery through interpolation on a graph Laplacian

2020 ◽  
Vol 636 ◽  
pp. A78 ◽  
Author(s):  
M. A. Schmitz ◽  
J.-L. Starck ◽  
F. Ngole Mboula ◽  
N. Auricchio ◽  
J. Brinchmann ◽  
...  
Keyword(s):  
Point Spread Function ◽  
Super Resolution ◽  
Statistical Error ◽  
Graph Laplacian ◽  
Weak Lensing ◽  
Shape Measurement ◽  
Model Errors ◽  
Point Spread ◽  
Spread Function ◽  
The Impact

Context. Future weak lensing surveys, such as the Euclid mission, will attempt to measure the shapes of billions of galaxies in order to derive cosmological information. These surveys will attain very low levels of statistical error, and systematic errors must be extremely well controlled. In particular, the point spread function (PSF) must be estimated using stars in the field, and recovered with high accuracy. Aims. The aims of this paper are twofold. Firstly, we took steps toward a nonparametric method to address the issue of recovering the PSF field, namely that of finding the correct PSF at the position of any galaxy in the field, applicable to Euclid. Our approach relies solely on the data, as opposed to parametric methods that make use of our knowledge of the instrument. Secondly, we studied the impact of imperfect PSF models on the shape measurement of galaxies themselves, and whether common assumptions about this impact hold true in an Euclid scenario. Methods. We extended the recently proposed resolved components analysis approach, which performs super-resolution on a field of under-sampled observations of a spatially varying, image-valued function. We added a spatial interpolation component to the method, making it a true 2-dimensional PSF model. We compared our approach to PSFEx, then quantified the impact of PSF recovery errors on galaxy shape measurements through image simulations. Results. Our approach yields an improvement over PSFEx in terms of the PSF model and on observed galaxy shape errors, though it is at present far from reaching the required Euclid accuracy. We also find that the usual formalism used for the propagation of PSF model errors to weak lensing quantities no longer holds in the case of an Euclid-like PSF. In particular, different shape measurement approaches can react differently to the same PSF modeling errors.

Super-resolution reconstruction of multiformat laser range-gated image by the estimate of point-spread function

2016 ◽  
Vol 9 (2) ◽  
pp. 226-233
Author(s):  
许廷发 XU Ting-fa ◽  
罗 璇 LUO Xuan ◽  
苏 畅 SU Chang ◽  
卞紫阳 BIAN Zi-yang
Keyword(s):  
Point Spread Function ◽  
Super Resolution ◽  
Laser Range ◽  
Point Spread ◽  
Spread Function

scholarly journals 3D Single-Molecule Super-Resolution Fluorescence Microscopy with the Corkscrew Point Spread Function

2016 ◽  
Vol 110 (3) ◽  
pp. 176a
Author(s):  
Maurice Lee ◽  
Matthew Lew ◽  
Alex von Diezmann ◽  
Lucien Weiss ◽  
Yoav Shechtman ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Point Spread Function ◽  
Super Resolution ◽  
Point Spread ◽  
Spread Function

scholarly journals Spherical Aberration of Point Spread Function with Asymmetric Pupil Mask

2016 ◽  
Vol 2016 ◽  
pp. 1-5
Author(s):  
Naresh Kumar Reddy Andra ◽  
Karuna Sagar Dasari
Keyword(s):  
Point Spread Function ◽  
Spherical Aberration ◽  
Spherical Wave ◽  
Super Resolution ◽  
Central Peak ◽  
Phase Mask ◽  
One Dimensional ◽  
Point Spread ◽  
Spread Function ◽  
Wave Aberration

Point spread function underneath spherical wave aberration with antiphase apodization has been obtained by one-dimensional pupil mask functions. In the presence of spherical aberration, suppression of optical side-lobes has increased on one side of the point spread function with the width of the periphery strips within the pupil mask. On introducing wave aberration effect, there exists dependence of the lateral resolution of central peak of the asymmetric point spread function on the amount of amplitude masking. However, the magnitude of intensity of central peak is originated be to amplified by the highest degree of amplitude and phase masking. Additionally, for aberrated asymmetric PSF, FWHM increases and it further decreases with the control parameters of amplitude and phase mask. The magnitude of this corollary can quantify the super resolution of diffracted structures under spherical aberration.

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