Mechanical Stimulation via Muscle Activity Is Necessary for the Maturation of Tendon Multiscale Mechanics During Embryonic Development

During embryonic development, tendons transform into a hypocellular tissue with robust tensile load-bearing capabilities. Previous work suggests that this mechanical transformation is due to increases in collagen fibril length and is dependent on mechanical stimulation via muscle activity. However, the relationship between changes in the microscale tissue structure and changes in macroscale tendon mechanics is still unclear. Additionally, the specific effect of mechanical stimulation on the multiscale structure-function relationships of developing tendons is also unknown. Therefore, the objective of this study was to measure the changes in tendon mechanics and structure at multiple length scales during embryonic development with and without skeletal muscle paralysis. Tensile testing of tendons from chick embryos was performed to determine the macroscale tensile modulus as well as the magnitude of the fibril strains and interfibrillar sliding with applied tissue strain. Embryos were also treated with either decamethonium bromide or pancuronium bromide to produce rigid or flaccid paralysis. Histology was performed to assess changes in tendon size, spacing between tendon subunits, and collagen fiber diameter. We found that the increase in the macroscale modulus observed with development is accompanied by an increase in the fibril:tissue strain ratio, which is consistent with an increase in collagen fibril length. Additionally, we found that flaccid paralysis reduced the macroscale tendon modulus and the fibril:tissue strain ratio, whereas less pronounced effects that were not statistically significant were observed with rigid paralysis. Finally, skeletal paralysis also reduced the size of collagen fibril bundles (i.e., fibers). Together, these data suggest that more of the applied tissue strain is transmitted to the collagen fibrils at later embryonic ages, which leads to an increase in the tendon macroscale tensile mechanics. Furthermore, our data suggest that mechanical stimulation during development is necessary to induce structural and mechanical changes at multiple physical length scales. This information provides valuable insight into the multiscale structure-function relationships of developing tendons and the importance of mechanical stimulation in producing a robust tensile load-bearing soft tissue.

Mechanical Stimulation via Muscle Activity is Necessary for the Maturation of Tendon Multiscale Mechanics during Embryonic Development

During embryonic development, tendons transform into a hypocellular tissue with robust tensile load-bearing capabilities. Previous work suggests that this mechanical transformation is due to increases in collagen fibril length and is dependent on mechanical stimulation via muscle activity. However, the relationship between changes in the microscale tissue structure and changes in macroscale tendon mechanics is still unclear. Additionally, the specific effect of mechanical stimulation on the multiscale structure-function relationships of developing tendons is also unknown. Therefore, the objective of this study was to measure the changes in tendon mechanics and structure at multiple length scales during embryonic development with and without skeletal muscle paralysis. Tensile testing of tendons from chicken embryos was performed to determine the macroscale tensile modulus as well as the magnitude of the fibril strains and interfibrillar sliding with applied tissue strain. Embryos were also treated with either decamethonium bromide or pancuronium bromide to produce rigid or flaccid paralysis. Histology was performed to assess changes in tendon size, spacing between tendon subunits, and collagen fiber diameter. We found that the increase in the macroscale modulus observed with development is accompanied by an increase in the fibril:tissue strain ratio, which is consistent with an increase in collagen fibril length. Additionally, we found that flaccid paralysis reduced the macroscale tendon modulus and the fibril:tissue strain ratio, whereas less pronounced effects that were not statistically significant were observed with rigid paralysis. Finally, skeletal paralysis also reduced the size of collagen fibril bundles (i.e., fibers). Together, these data suggest that more of the applied tissue strain is transmitted to the collagen fibrils at later embryonic ages, which leads in an increase the tendon macroscale tensile mechanics. Furthermore, our data suggest that mechanical stimulation during development is necessary to induce structural and mechanical changes at multiple physical length scales. This information provides valuable insight into the multiscale structure-function relationships of developing tendons and the importance of mechanical stimulation in producing a robust tensile load-bearing soft tissue.

Sources of Error When Measuring Achilles Tendon Mechanics During the Stance Phase of Running

In recent years, the use of methods that combine motion capture with ultrasound (MoCapUs) has increased. Although several limitations and individual errors of these methods have been reported, the total error from all the potential sources together has not been estimated. The aim of this study was to establish the total error in the Achilles tendon (AT) measurements, specifically its length (ATL), strain (ATS) and moment arm (ATMA) acquired with MoCapUs during running. The total error from digitising, marker movement, ultrasound calibration and probe rotation errors caused mean ATL error of 4.2 ± 0.6 mm, mean ATMA error of 0.1 ± 0.1 mm, and could potentially alter measured ATS by a mean 2.9 ± 0.2 %. Correcting the calcaneus insertion position (CIP) and properly synchronising ultrasound and motion capture data combined caused ATL and ATMA changes up to 5.4 ± 1.7 mm and 11.6 ± 1.3 mm, respectively. Changes in ATL and ATS due to the CIP correction and synchronisation individually were similar. However, the ATMA change was almost exclusively due to the CIP correction. Finally, if all sources of error were combined, the total ATL error could reach 13.1 mm, the total ATMA error could reach 14.4 mm, and ATS differences could reach up to ± 6.7%. The magnitude such errors emphasises the fact that MoCapUS based AT measurements must be interpreted within the scope of their corresponding errors.

Effect of Achilles Tendon Mechanics on the Running Economy of Elite Endurance Athletes

The Achilles tendon stores and releases strain energy, influencing running economy. The present study aims to verify the influence of the Achilles tendon tangent modulus, as a material property, on running economy by comparing two groups of elite endurance-performance athletes undergoing different running training volumes. Twelve athletes, six long-distance runners and six pentathletes, were studied. Long-distance runners had a higher weekly running training volume (116.7±13.7 vs. 58.3±20.4 km, p<0.05) and a better running economy (204.3±12.0 vs. 222.0±8.7 O2 mL ∙ kg−1 ∙ km−1, p<0.05) evaluated in a treadmill at 16 km·h–1, 1% inclination. Both groups presented similar VO2max (68.5±3.8 vs. 65.7±5.0 mL ∙ min−1 ∙ kg−1, p>0.05). Achilles tendon tangent modulus was estimated from ultrasound-measured deformations, with the ankle passively mobilized by a dynamometer. True stress was calculated from the measured torque. The long-distance runners had a higher maximum tangent modulus (380.6±92.2 vs. 236.2±82.6 MPa, p<0.05) and maximum true stress than pentathletes (24.2±5.1 vs. 16.0±3.5 MPa, p<0.05). The correlation coefficient between tangent modulus at larger deformations was R=–0.7447 (p<0.05). Quantifying tendon tissue adaptations associated with different running training volumes will support subject and modality-specific workouts prescription of elite endurance athletes.

Peculiarities of Muscle-Tendon Mechanics and Energetics in Elite Athletes in Various Sports

The article presents results of the research on jumping strategies applied by elite athletes in various sport disciplines. Research hypothesis: to perform the same motor task athletes employ different ways of organizing the movement and different features of MTU functioning. The choice of a mechanism to enhance muscle contraction depends on sport discipline, in particular specific features of the sport movement. The study involved members of the Russian national teams in alpine skiing, bobsleighing, mogul skiing and ski jumping. The athletes performed drop jumps from the heights of 0.1, 0.3, and 0.5 m with no arm swing. Experimental data were obtained online from 24 cameras using the Qualisys motion capture system (400 frames per second) and the two force plates AMTI 6000. Data was processed using the OpenSim package. The authors calculated the amount of accumulation and utilization of elastic strain energy and assessed metabolic energy expenditures in MTU. The authors concluded that employment of different strategies of movement organization in drop jumps could be explained by the transfer of motor skills specific to the athlete’s sport discipline. The results of the study may help coaches develop individual training plans for athletes, in particular strength training exercises targeting specific muscle groups.

Contribution of Achilles tendon mechanical properties to torque steadiness in persons with transfemoral amputation

Background: How Achilles tendon mechanics and plantar flexion strength and torque steadiness are altered in the intact leg of persons with trauma-related amputation is unknown. Understanding Achilles tendon mechanics following amputation will further inform rehabilitation approaches to enhance posture, balance, and force control. Objective: Conduct a pilot study to quantify plantar flexion maximal voluntary contraction torque, torque steadiness, and Achilles tendon mechanics in persons with unilateral trauma-related transfemoral amputation and controls without amputation. Study design: Cross-sectional study. Methods: Isometric plantar flexion maximal voluntary contractions were performed with the intact leg of ten males with transfemoral amputation (48 ± 14 years) and the dominant leg of age-matched male controls without amputation. Torque steadiness was calculated as the coefficient of variation in torque over 6 s during submaximal tracking tasks (5%, 10%, 25%, 50%, and 75% maximal voluntary contraction). Achilles tendon elongation and cross-sectional area were recorded with ultrasound to calculate strain, stress, and stiffness. Results: Maximal voluntary contraction and torque steadiness did not differ between persons with amputation (90.6 ± 31.6 N m, 3.7 ± 2.0%) and controls (95.8 ± 26.8 N m, 2.9 ± 1.2%; p > 0.05). Tendon stiffness (21.1 ± 18.2 N/mm) and strain (5.2 ± 1.3%) did not differ between groups ( p > 0.05). Tendon cross-sectional area was 10% greater in persons with amputation leading to 29% lower stress ( p = 0.021). Maximal voluntary contraction was a predictor of a lower coefficient of variation in torque ( R2 = 0.11, p < 0.05). Conclusion: Persons with trauma-related transfemoral amputation do not differ in plantar flexion maximal voluntary contraction and torque steadiness of the intact leg compared with controls without amputation. Larger tendon cross-sectional area reduces stress and enables distribution of force across a greater area.

Elastase treatment of tendon specifically impacts the mechanical properties of the interfascicular matrix

The tendon interfascicular matrix (IFM) binds tendon fascicles together. As a result of its low stiffness behaviour under small loads, it enables non-uniform loading and increased overall extensibility of tendon by facilitating fascicle sliding. This function is particularly important in energy storing tendons, with previous studies demonstrating enhanced extensibility, recovery and fatigue resistance in the IFM of energy storing compared to positional tendons. However, the compositional specialisations within the IFM that confer this behaviour remain to be elucidated. It is well established that the IFM is rich in elastin, therefore we sought to test the hypothesis that elastin depletion (following elastase treatment) will significantly impact IFM, but not fascicle, mechanical properties, reducing IFM resilience in all samples, but to a greater extent in younger tendons, which have a higher elastin content. Using a combination of quasi-static and fatigue testing, and optical imaging, we confirmed our hypothesis, demonstrating that elastin depletion resulted in significant decreases in IFM viscoelasticity, fatigue resistance and recoverability compared to untreated samples, with no significant changes to fascicle mechanics. Ageing had little effect on fascicle or IFM response to elastase treatment.This study offers a first insight into the functional importance of elastin in regional specific tendon mechanics. It highlights the important contribution of elastin to IFM mechanical properties, demonstrating that maintenance of a functional elastin network within the IFM is essential to maintain IFM and thus tendon integrity.

Importance of Maximal Strength and Muscle-Tendon Mechanics for Improving Force Steadiness in Persons with Parkinson’s Disease

Although plantar flexion force steadiness (FS) is reduced in persons with Parkinson’s disease (PD), the underlying causes are unknown. The aim of this exploratory design study was to ascertain the influence of maximal voluntary contraction (MVC) force and gastrocnemius-Achilles muscle-tendon unit behaviour on FS in persons with PD. Nine persons with PD and nine age- and sex-matched non-PD controls (~70 years, 6 females per group) performed plantar flexion MVCs and sub-maximal tracking tasks at 5, 10, 25, 50 and 75% MVC. Achilles tendon elongation and medial gastrocnemius fascicle lengths were recorded via ultrasound during contraction. FS was quantified using the coefficient of variation (CV) of force. Contributions of MVC and tendon mechanics to FS were determined using multiple regression analyses. Persons with PD were 35% weaker during MVC (p = 0.04) and had 97% greater CV (p = 0.01) with 47% less fascicle shortening (p = 0.004) and 38% less tendon elongation (p = 0.002) than controls. Reduced strength was a direct contributor to lower FS in PD (ß = 0.631), and an indirect factor through limiting optimal muscle-tendon unit interaction. Interestingly, our findings indicate an uncoupling between fascicle shortening and tendon elongation in persons with PD. To better understand limitations in FS and muscle-tendon unit behavior, it is imperative to identify the origins of MVC decrements in persons with PD.