scholarly journals Multimodal optical imaging system for in vivo investigation of cerebral oxygen delivery and energy metabolism

2015 ◽  
Vol 6 (12) ◽  
pp. 4994 ◽  
Author(s):  
Mohammad A. Yaseen ◽  
Vivek J. Srinivasan ◽  
Iwona Gorczynska ◽  
James G. Fujimoto ◽  
David A. Boas ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Optical Imaging ◽  
Oxygen Delivery ◽  
Imaging System ◽  
Cerebral Oxygen ◽  
Optical Imaging System

scholarly journals The Development and Applications of a Dual Optical Imaging System for Studying Glioma Stem Cells

2019 ◽  
Vol 18 ◽  
pp. 153601211987089 ◽  
Author(s):  
Po-An Tai ◽  
Yen-Lin Liu ◽  
Ya-Ting Wen ◽  
Chien-Min Lin ◽  
Thanh-Tuan Huynh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Brain Tumor ◽  
Optical Imaging ◽  
Fluorescent Protein ◽  
Imaging System ◽  
High Rate ◽  
Glioma Stem Cells ◽  
Optical Imaging System

Glioblastoma multiforme represents one of the deadliest brain tumor types, manifested by a high rate of recurrence and poor prognosis. The presence of glioma stem cells (GSCs) can repopulate the tumor posttreatment and resist therapeutics. A better understanding of GSC biology is essential for developing more effective interventions. We established a CD133 promoter-driven dual reporter, expressing green fluorescent protein (GFP) and firefly luciferase (CD133-LG), capable for in vitro and in vivo imaging of CD133+ GSCs. We first demonstrated the reporter enabled in vitro analyses of GSCs. DBTRG-05MG (Denver Brain Tumor Research Group 05) carrying CD133-LG (DBTRG-05MG-CD133-LG) system reported increased GFP/luciferase activities in neurospheres. Additionally, we identified and isolated CD133+/GFP+ cells with increased tumorigenic properties, stemness markers, Notch1, β-catenin, and Bruton’s tyrosine kinase (Btk). Furthermore, prolonged temozolomide (TMZ) treatment enriched GSCs (reflected by increased percentage of CD133+ cells). Subsequently, Btk inhibitor, ibrutinib, suppressed GSC generation and stemness markers. Finally, we demonstrated real-time evaluation of anti-GSC function of ibrutinib in vivo with TMZ-enriched GSCs. Tumorigenesis was noninvasively monitored by bioluminescence imaging and mice that received ibrutinib showed a significantly lower tumor burden, indicating ibrutinib as a potential GSC inhibitor. In conclusion, we established a dual optical imaging system which enables the identification of CD133+ GSCs and screening for anti-GSC drugs.

scholarly journals A Multimode Optical Imaging System for Preclinical Applications In Vivo: Technology Development, Multiscale Imaging, and Chemotherapy Assessment

2011 ◽  
Vol 14 (4) ◽  
pp. 431-442 ◽  
Author(s):  
Jae Youn Hwang ◽  
Sebastian Wachsmann-Hogiu ◽  
V. Krishnan Ramanujan ◽  
Julia Ljubimova ◽  
Zeev Gross ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Technology Development ◽  
Imaging System ◽  
Optical Imaging System ◽  
Multiscale Imaging

1206: In vivo Visual Monitoring of Implanted Embryonic Stem Cells in Rat Corpus Cavernosum Using Optical Imaging System

2006 ◽  
Vol 175 (4S) ◽  
pp. 388-388
Author(s):  
Kwangsung Park ◽  
Jung-Joon Min ◽  
Kyung Jin Oh ◽  
In Sang Hwang ◽  
Hyun Sook Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Optical Imaging ◽  
Imaging System ◽  
Embryonic Stem ◽  
Corpus Cavernosum ◽  
Visual Monitoring ◽  
Optical Imaging System

scholarly journals Percutaneous optical imaging system to track reporter gene expression from vasculatures in vivo

2006 ◽  
Vol 11 (3) ◽  
pp. 034008 ◽  
Author(s):  
S. Kar ◽  
A. Kumar ◽  
F. Gao ◽  
B. Qiu ◽  
X. Zhan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Optical Imaging ◽  
Reporter Gene ◽  
Imaging System ◽  
Reporter Gene Expression ◽  
Optical Imaging System

scholarly journals Dual-mode optical imaging system for fluorescence image-guided surgery

2014 ◽  
Vol 39 (13) ◽  
pp. 3830 ◽  
Author(s):  
Nan Zhu ◽  
Suman Mondal ◽  
Shengkui Gao ◽  
Samuel Achilefua ◽  
Viktor Gruev ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Imaging System ◽  
Image Guided Surgery ◽  
Dual Mode ◽  
Fluorescence Image ◽  
Guided Surgery ◽  
Image Guided ◽  
Optical Imaging System
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