scholarly journals Percutaneous optical imaging system to track reporter gene expression from vasculatures in vivo

2006 ◽  
Vol 11 (3) ◽  
pp. 034008 ◽  
Author(s):  
S. Kar ◽  
A. Kumar ◽  
F. Gao ◽  
B. Qiu ◽  
X. Zhan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Optical Imaging ◽  
Reporter Gene ◽  
Imaging System ◽  
Reporter Gene Expression ◽  
Optical Imaging System

scholarly journals Multimodal optical imaging system for in vivo investigation of cerebral oxygen delivery and energy metabolism

2015 ◽  
Vol 6 (12) ◽  
pp. 4994 ◽  
Author(s):  
Mohammad A. Yaseen ◽  
Vivek J. Srinivasan ◽  
Iwona Gorczynska ◽  
James G. Fujimoto ◽  
David A. Boas ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Optical Imaging ◽  
Oxygen Delivery ◽  
Imaging System ◽  
Cerebral Oxygen ◽  
Optical Imaging System

scholarly journals In Vivo Analysis of Glucocorticoid-Induced Reporter Gene Expression Using Gene Gun DNA Delivery

2002 ◽  
Vol 25 (8) ◽  
pp. 1115-1118 ◽  
Author(s):  
Kiyoshi Tanigawa ◽  
Katsunao Tanaka ◽  
Hidetaka Nagase ◽  
Hidekazu Miyake ◽  
Mamoru Kiniwa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reporter Gene ◽  
Dna Delivery ◽  
Reporter Gene Expression ◽  
Gene Gun ◽  
In Vivo Analysis

Comparison of Vibrio and Firefly Luciferases as Reporter Gene Systems for Use in Bacteria and Plants

1996 ◽  
Vol 23 (1) ◽  
pp. 75 ◽  
Author(s):  
SR Mudge ◽  
WR Lewis-Henderson ◽  
RG Birch
Keyword(s):  
Gene Expression ◽  
Reporter Gene ◽  
Ccd Camera ◽  
Reporter Gene Expression ◽  
In Vitro Assays ◽  
E Coli ◽  
Cell Extracts ◽  
Cooled Ccd

Luciferase genes from Vibrio harveyi (luxAB) and firefly (luc) were introduced into E. coli, Agrobacteriurn, Arabidopsis and tobacco. Transformed bacteria and plants were quantitatively assayed for luciferase activity using a range of in vitro and in vivo assay conditions. Both lux and luc proved efficient reporter genes in bacteria, although it is important to be aware that the sensitive assays may detect expression due to readthrough from distant promoters. LUX activity was undetectable by liquid nitrogen-cooled CCD camera assays on intact tissues of plants which showed strong luxAB expression by in vitro assays. The decanal substrate for the lux assay was toxic to many plant tissues, and caused chemiluminescence in untransformed Arabidopsis leaves. These are serious limitations to application of the lux system for sensitive, non-toxic assays of reporter gene expression in plants. In contrast, LUC activity was readily detectable in intact tissues of all plants with luc expression detectable by luminometer assays on cell extracts. Image intensities of luc-expressing leaves were commonly two to four orders of magnitude above controls under the CCD camera. Provided adequate penetration of the substrate luciferin is obtained, luc is suitable for applications requiring sensitive, non-toxic assays of reporter gene expression in plants.

scholarly journals The Development and Applications of a Dual Optical Imaging System for Studying Glioma Stem Cells

2019 ◽  
Vol 18 ◽  
pp. 153601211987089 ◽  
Author(s):  
Po-An Tai ◽  
Yen-Lin Liu ◽  
Ya-Ting Wen ◽  
Chien-Min Lin ◽  
Thanh-Tuan Huynh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Brain Tumor ◽  
Optical Imaging ◽  
Fluorescent Protein ◽  
Imaging System ◽  
High Rate ◽  
Glioma Stem Cells ◽  
Optical Imaging System

Glioblastoma multiforme represents one of the deadliest brain tumor types, manifested by a high rate of recurrence and poor prognosis. The presence of glioma stem cells (GSCs) can repopulate the tumor posttreatment and resist therapeutics. A better understanding of GSC biology is essential for developing more effective interventions. We established a CD133 promoter-driven dual reporter, expressing green fluorescent protein (GFP) and firefly luciferase (CD133-LG), capable for in vitro and in vivo imaging of CD133+ GSCs. We first demonstrated the reporter enabled in vitro analyses of GSCs. DBTRG-05MG (Denver Brain Tumor Research Group 05) carrying CD133-LG (DBTRG-05MG-CD133-LG) system reported increased GFP/luciferase activities in neurospheres. Additionally, we identified and isolated CD133+/GFP+ cells with increased tumorigenic properties, stemness markers, Notch1, β-catenin, and Bruton’s tyrosine kinase (Btk). Furthermore, prolonged temozolomide (TMZ) treatment enriched GSCs (reflected by increased percentage of CD133+ cells). Subsequently, Btk inhibitor, ibrutinib, suppressed GSC generation and stemness markers. Finally, we demonstrated real-time evaluation of anti-GSC function of ibrutinib in vivo with TMZ-enriched GSCs. Tumorigenesis was noninvasively monitored by bioluminescence imaging and mice that received ibrutinib showed a significantly lower tumor burden, indicating ibrutinib as a potential GSC inhibitor. In conclusion, we established a dual optical imaging system which enables the identification of CD133+ GSCs and screening for anti-GSC drugs.

scholarly journals Optical Imaging of Cardiac Reporter Gene Expression in Living Rats

Circulation ◽  
2002 ◽  
Vol 105 (14) ◽  
pp. 1631-1634 ◽  
Author(s):  
Joseph C. Wu ◽  
Masayuki Inubushi ◽  
Gobalakrishnan Sundaresan ◽  
Heinrich R. Schelbert ◽  
Sanjiv S. Gambhir
Keyword(s):  
Gene Expression ◽  
Optical Imaging ◽  
Reporter Gene ◽  
Reporter Gene Expression
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