scholarly journals Label-free non-invasive Subwavelength-resolution imaging using Yeast Cells as biological lenses

2021 ◽  
Author(s):  
Chunlei Jiang ◽  
Hangyu Yue ◽  
Bing Yan ◽  
taiji Dong ◽  
Xiangyu Cui ◽  
...  
Keyword(s):  
Yeast Cells ◽  
Label Free ◽  
Non Invasive ◽  
Subwavelength Resolution ◽  
Resolution Imaging

Label-free non-invasive classification of rice seeds using optical coherence tomography assisted with deep neural network

2021 ◽  
Vol 137 ◽  
pp. 106861
Author(s):  
Deepa Joshi ◽  
Ankit Butola ◽  
Sheetal Raosaheb Kanade ◽  
Dilip K. Prasad ◽  
S.V. Amitha Mithra ◽  
...  
Keyword(s):  
Neural Network ◽  
Optical Coherence Tomography ◽  
Deep Neural Network ◽  
Optical Coherence ◽  
Label Free ◽  
Non Invasive ◽  
Rice Seeds

scholarly journals Non-Invasive Identification of Nutrient Components in Grain

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3124
Author(s):  
Charles Farber ◽  
A. S. M. Faridul Islam ◽  
Endang M. Septiningsih ◽  
Michael J. Thomson ◽  
Dmitry Kurouski
Keyword(s):  
Rice Variety ◽  
Nutrient Content ◽  
Plant Diseases ◽  
Variety Identification ◽  
Successful Implementation ◽  
Label Free ◽  
Accurate Identification ◽  
Analytical Technique ◽  
Non Invasive ◽  
Digital Farming

Digital farming is a modern agricultural concept that aims to maximize the crop yield while simultaneously minimizing the environmental impact of farming. Successful implementation of digital farming requires development of sensors to detect and identify diseases and abiotic stresses in plants, as well as to probe the nutrient content of seeds and identify plant varieties. Experimental evidence of the suitability of Raman spectroscopy (RS) for confirmatory diagnostics of plant diseases was previously provided by our team and other research groups. In this study, we investigate the potential use of RS as a label-free, non-invasive and non-destructive analytical technique for the fast and accurate identification of nutrient components in the grains from 15 different rice genotypes. We demonstrate that spectroscopic analysis of intact rice seeds provides the accurate rice variety identification in ~86% of samples. These results suggest that RS can be used for fully automated, fast and accurate identification of seeds nutrient components.

scholarly journals Tethered Lipid Membranes as a Nanoscale Arrangement Towards Non-Invasive Analysis of Acute Pancreatitis

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 755
Author(s):  
Rima Budvytyte ◽  
Akvile Milasiute ◽  
Dalius Vitkus ◽  
Kestutis Strupas ◽  
Aiste Gulla ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Lipid Membranes ◽  
Electrochemical Impedance ◽  
Membrane Integrity ◽  
Electrochemical Technique ◽  
Label Free ◽  
Non Invasive ◽  
Novel Approach ◽  
Analytical System ◽  
Shock Proteins

Extracellular heat shock proteins (HSPs) mediate immunological functions and are involved in pathologies such as infection, stress, and cancer. Here, we demonstrated the dependence of an amount of HSP70 and HSP90 in serum vs. severity of acute pancreatitis (AP) on a cohort of 49 patients. Tethered bilayer lipid membranes (tBLMs) have been developed to investigate HSPs’ interactions with tBLMs that can be probed by electrochemical impedance spectroscopy (EIS). The results revealed that HSP70 and HSP90 interact via different mechanisms. HSP70 shows the damage of the membrane, while HSP90 increases the insulation properties of tBLM. These findings provide evidence that EIS offers a novel approach for the study of the changes in membrane integrity induced by HSPs proteins. Herein, we present an alternative electrochemical technique, without any immunoprobes, that allows for the monitoring of HSPs on nanoscaled tBLM arrangement in biologics samples such us human urine. This study demonstrates the great potential of tBLM to be used as a membrane based biosensor for novel, simple, and non-invasive label-free analytical system for the prediction of AP severity.

scholarly journals Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo

2010 ◽  
Vol 35 (19) ◽  
pp. 3195 ◽  
Author(s):  
Chi Zhang ◽  
Konstantin Maslov ◽  
Lihong V. Wang
Keyword(s):  
Optical Absorption ◽  
Photoacoustic Microscopy ◽  
Label Free ◽  
Subwavelength Resolution

Non-invasive label-free investigation and typing of head and neck cancers by multimodal nonlinear microscopy

2012 ◽  
Author(s):  
Tobias Meyer ◽  
Nadine Vogler ◽  
Benjamin Dietzek ◽  
Denis Akimov ◽  
Johanna Inhestern ◽  
...  
Keyword(s):  
Head And Neck ◽  
Head And Neck Cancers ◽  
Nonlinear Microscopy ◽  
Label Free ◽  
Non Invasive

Non-invasive and label-free identification of human natural killer subclasses by biophysical single-cell features in microfluidic flow

Lab on a Chip ◽  
2021 ◽  
Author(s):  
David Dannhauser ◽  
Domenico Rossi ◽  
Anna T Palatucci ◽  
Valentina Rubino ◽  
Flavia Carriero ◽  
...  
Keyword(s):  
Single Cell ◽  
Natural Killer ◽  
Label Free ◽  
Therapeutic Treatment ◽  
Non Invasive ◽  
Microfluidic Flow

Natural Killer (NK) are indicated as favorite candidates for innovative therapeutic treatment and are divided in two subclasses: immature regulatory NK CD56bright and mature cytotoxic NK CD56dim. Therefore, the ability...

Label-free RF biosensors for human cell dielectric spectroscopy

2009 ◽  
Vol 1 (6) ◽  
pp. 497-504 ◽  
Author(s):  
Claire Dalmay ◽  
Arnaud Pothier ◽  
Mathilde Cheray ◽  
Fabrice Lalloue ◽  
Marie-Odile Jauberteau ◽  
...  
Keyword(s):  
Human Cell ◽  
Cell Types ◽  
Label Free ◽  
Microwave Frequencies ◽  
Non Invasive ◽  
System Cell ◽  
Electromagnetic Characteristics ◽  
Biosensor Chip ◽  
Rf Signal

This paper presents an original biosensor chip allowing determination of intrinsic relative permittivity of biological cells at microwave frequencies. This sensor permits non-invasive cell identification and discrimination using an RF signal to probe intracellular medium of biological samples. Indeed, these sensors use an RF planar resonator that allows detection capabilities on less than 10 cells, thanks to the microscopic size of its sensitive area. Especially, measurements between 15 and 35 GHz show the ability label-free biosensors to differentiate two human cell types using their own electromagnetic characteristics. The real part of permittivity of cells changes from 20 to 48 for the nervous system cell types studied. The proposed biodetection method is detailed and we show how the accuracy and the repeatability of measurements have been improved to reach reproducible measurements.

scholarly journals Pinhole microLED Array as Point Source Illumination for Miniaturized Lensless Cell Monitoring Systems

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 866 ◽  
Author(s):  
Shinta Mariana ◽  
Gregor Scholz ◽  
Feng Yu ◽  
Agus Budi Dharmawan ◽  
Iqbal Syamsu ◽  
...  
Keyword(s):  
Image Quality ◽  
Light Emitting Diode ◽  
Free Cell ◽  
Monitoring Systems ◽  
Label Free ◽  
Light Emitting ◽  
Non Invasive ◽  
Cell Monitoring ◽  
Blue Spectral Region ◽  
Led Arrays

Pinhole‐shaped light‐emitting diode (LED) arrays with dimension ranging from 100 μm down to 5 μm have been developed as point illumination sources. The proposed microLED arrays, which are based on gallium nitride (GaN) technology and emitting in the blue spectral region (λ = 465 nm), are integrated into a compact lensless holographic microscope for a non‐invasive, label‐free cell sensing and imaging. From the experimental results using single pinhole LEDs having a diameter of 90 μm, the reconstructed images display better resolution and enhanced image quality compared to those captured using a commercial surface‐mount device (SMD)‐based LED.

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