scholarly journals High-speed adaptive optics line-scan OCT for cellular-resolution optoretinography

2020 ◽  
Vol 11 (9) ◽  
pp. 5274 ◽  
Author(s):  
Vimal Prabhu Pandiyan ◽  
Xiaoyun Jiang ◽  
Aiden Maloney-Bertelli ◽  
James A. Kuchenbecker ◽  
Utkarsh Sharma ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
High Speed ◽  
Line Scan ◽  
Cellular Resolution

Adaptive optics line-scan OCT for high-speed imaging of retinal structure and function (Conference Presentation)

2020 ◽  
Author(s):  
Vimal Prabhu Pandiyan ◽  
Aiden M. Bertelli ◽  
James Kuchenbecker ◽  
Kevin C. Boyle ◽  
Tong Ling ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
High Speed ◽  
Structure And Function ◽  
High Speed Imaging ◽  
Line Scan ◽  
Retinal Structure ◽  
And Function

scholarly journals High-speed adaptive optics line scan confocal retinal imaging for human eye

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0169358 ◽  
Author(s):  
Jing Lu ◽  
Boyu Gu ◽  
Xiaolin Wang ◽  
Yuhua Zhang
Keyword(s):  
Adaptive Optics ◽  
High Speed ◽  
Retinal Imaging ◽  
Human Eye ◽  
Line Scan

scholarly journals High-speed adaptive optics line-scan OCT for cellular-resolution optoretinography

2020 ◽  
Author(s):  
Vimal Prabhu Pandiyan ◽  
Xiaoyun Jiang ◽  
Aiden Maloney Bertelli ◽  
James A. Kuchenbecker ◽  
Utkarsh Sharma ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Adaptive Optics ◽  
Phase Stability ◽  
High Speed ◽  
Collection Efficiency ◽  
Spatial Scales ◽  
Widespread Application ◽  
Parallel Acquisition ◽  
Line Scan ◽  
Length Changes

AbstractOptoretinography – the non-invasive, optical imaging of light-induced functional activity in the retina – stands to provide a critical biomarker for testing the safety and efficacy of new therapies as well as their rapid translation to the clinic. Optical phase change in response to light, as readily accessible in phase-resolved OCT, offers a path towards all-optical imaging of retinal function. However, typical human eye motion adversely affects phase stability and precludes the recording of fast light-induced retinal events. Here we introduce a high-speed line-scan spectral domain OCT with adaptive optics (AO), aimed at volumetric imaging and phase-resolved acquisition of retinal responses to light. By virtue of parallel acquisition of an entire retinal cross-section (B-scan) in a single high-speed camera frame, depth-resolved tomograms at speeds up to 16 kHz were achieved with high sensitivity and phase stability. To optimize spectral and spatial resolution, an anamorphic detection paradigm was introduced enabling improved light collection efficiency and signal roll-off compared to traditional methods. The benefits in speed, resolution and sensitivity were exemplified in imaging nanometer-millisecond scale light-induced optical path length changes in cone photoreceptor outer segments. With 660 nm stimuli, individual cone responses readily segregated into three clusters, corresponding to long, middle and short-wavelength cones. Recording such optoretinograms on spatial scales ranging from individual cones, to 100 μm-wide retinal patches offers a robust and sensitive biomarker for cone function in health and disease. Furthermore, incorporating this capability into an easy-to-use and ubiquitous diagnostic platform of OCT enables its widespread application to patient care and drug development.

scholarly journals A micromirror array with annular partitioning for high-speed random-access axial focusing

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Nathan Tessema Ersumo ◽  
Cem Yalcin ◽  
Nick Antipa ◽  
Nicolas Pégard ◽  
Laura Waller ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Visual Information ◽  
High Speed ◽  
Random Access ◽  
General Purpose ◽  
Phase Shifting ◽  
Optical Systems ◽  
Piston Motion ◽  
Refresh Rate ◽  
Circular Symmetry

Abstract Dynamic axial focusing functionality has recently experienced widespread incorporation in microscopy, augmented/virtual reality (AR/VR), adaptive optics and material processing. However, the limitations of existing varifocal tools continue to beset the performance capabilities and operating overhead of the optical systems that mobilize such functionality. The varifocal tools that are the least burdensome to operate (e.g. liquid crystal, elastomeric or optofluidic lenses) suffer from low (≈100 Hz) refresh rates. Conversely, the fastest devices sacrifice either critical capabilities such as their dwelling capacity (e.g. acoustic gradient lenses or monolithic micromechanical mirrors) or low operating overhead (e.g. deformable mirrors). Here, we present a general-purpose random-access axial focusing device that bridges these previously conflicting features of high speed, dwelling capacity and lightweight drive by employing low-rigidity micromirrors that exploit the robustness of defocusing phase profiles. Geometrically, the device consists of an 8.2 mm diameter array of piston-motion and 48-μm-pitch micromirror pixels that provide 2π phase shifting for wavelengths shorter than 1100 nm with 10–90% settling in 64.8 μs (i.e., 15.44 kHz refresh rate). The pixels are electrically partitioned into 32 rings for a driving scheme that enables phase-wrapped operation with circular symmetry and requires <30 V per channel. Optical experiments demonstrated the array’s wide focusing range with a measured ability to target 29 distinct resolvable depth planes. Overall, the features of the proposed array offer the potential for compact, straightforward methods of tackling bottlenecked applications, including high-throughput single-cell targeting in neurobiology and the delivery of dense 3D visual information in AR/VR.

High-speed high-resolution color line scan camera for machine vision

1993 ◽  
Author(s):  
David J. Litwiller ◽  
Mike Miethig ◽  
Brian C. Doody ◽  
P. Tom Jenkins
Keyword(s):  
High Resolution ◽  
Machine Vision ◽  
High Speed ◽  
Color Line ◽  
Line Scan

scholarly journals High-speed, cortex-wide volumetric recording of neuroactivity at cellular resolution using light beads microscopy

2021 ◽  
Vol 18 (9) ◽  
pp. 1103-1111 ◽  
Author(s):  
Jeffrey Demas ◽  
Jason Manley ◽  
Frank Tejera ◽  
Kevin Barber ◽  
Hyewon Kim ◽  
...  
Keyword(s):  
High Speed ◽  
Cellular Resolution

High-speed high-resolution color line scan camera for machine vision

1994 ◽  
Author(s):  
David J. Litwiller ◽  
Mike Miethig ◽  
Brian C. Doody ◽  
P. Tom Jenkins
Keyword(s):  
High Resolution ◽  
Machine Vision ◽  
High Speed ◽  
Color Line ◽  
Line Scan
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