scholarly journals Evaluation of Reference Genes for Differential Gene Expression Study of Bovine Tuberculosis

2017 ◽  
Vol 5 (2) ◽  
pp. 17-24
Author(s):  
Ailam Lim ◽  
Steven Bolin
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Bovine Tuberculosis ◽  
Reference Genes ◽  
Gene Expression Study ◽  
Expression Study ◽  
Differential Gene

scholarly journals Comparative gene expression study between two turmeric (Curcuma longa L.) cultivars

1970 ◽  
Vol 27 (2) ◽  
pp. 131
Author(s):  
D K Ayer, K G Modha, V B Parekh, R K Patel, V Ramtekey, A P Bhuriya
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
High Performance ◽  
Curcuma Longa ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Gene Expression Study ◽  
Expression Study ◽  
Opposite Pattern ◽  
Differential Gene

Two turmeric (Curcuma longa L.) cultivars differing in curcumin content viz GNT-2 (4.6 % curcumin) and NDH-98 (1.6% curcumin) were selected for comparative gene expression study in association with total curcumin contents. Sampling was done at six months after planting in open field condition. Differential gene expression patterns were observed between two cultivars by reverse transcription quantitative real time polymerase chain reaction (RT-qPCR), and total curcumin contents were quantified using high performance liquid chromatography (HPLC). Low curcumin yielding cultivar, NDH-98, exhibited higher expression of DCS and CURS3 whereas lower expression of CURS1 and CURS2. However, opposite pattern was observed in a high curcumin yielding cultivar, GNT-2, where DCS and CURS3 expressions were lower but CURS1 and CURS2 expressions were higher. CURS3 showed similar expression between both cultivars. CURS1 and CURS2 expression patterns showed more closer association than DCS and CURS3 gene expression patterns with each other. Differential gene expression patterns could be predictively associated with differential curcuminoids concentrations in turmeric cultivars.

Differential Gene Expression for Age Estimation of Forensically Important Sarcophaga peregrina (Diptera: Sarcophagidae) Intrapuparial

2019 ◽  
Vol 57 (1) ◽  
pp. 65-77 ◽  
Author(s):  
Yanjie Shang ◽  
Lipin Ren ◽  
Li Yang ◽  
Shiwen Wang ◽  
Wei Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Age Estimation ◽  
Reference Genes ◽  
Morphological Changes ◽  
Expression Profiles ◽  
Quantitative Polymerase Chain Reaction ◽  
Accurate Determination ◽  
28S Rrna ◽  
Differential Gene

Abstract Sarcophaga peregrina is an important flesh fly species for estimating the minimum postmortem interval (PMImin) in forensic entomology. The accurate determination of the developmental age is a crucial task for using necrophagous sarcophagids to estimate PMImin. During larval development, the age determination is straight forward by the morphological changes and variation of length, weight, and width; however, the age estimation of sarcophagid intrapuparial is more difficult due to anatomical and morphological changes not being visible. The analysis of differentially expressed genes (DEGs) during sarcophagid metamorphosis is a potential method for age estimation of intrapuparial. In the present study, real-time quantitative polymerase chain reaction (RT-qPCR) was used to analyze the differential gene expression level of S. peregrina intrapuparial in different constant temperatures (35°C, 25°C, and 15°C). In addition, the appropriate reference genes of S. peregrina were selected in the intrapuparial and at different temperatures to obtain reliable and valid gene expression profiles. The results indicated that two candidate genes (18S rRNA and 28S rRNA) were the most reliable reference genes, and four DEGs (Hsp90, A-alpha, AFP, AFBP) have the potential to be used to more accuracy estimate the age of S. peregrina intrapuparial.

scholarly journals Critical selection of reliable reference genes for gene expression study in the HepaRG cell line

2011 ◽  
Vol 81 (10) ◽  
pp. 1255-1261 ◽  
Author(s):  
Liesbeth Ceelen ◽  
Ward De Spiegelaere ◽  
Michael David ◽  
Jurgen De Craene ◽  
Mathieu Vinken ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Reference Genes ◽  
Gene Expression Study ◽  
Expression Study ◽  
Heparg Cell ◽  
Heparg Cell Line ◽  
Selection Of

scholarly journals Selection of reliable reference genes for gene expression study in nasopharyngeal carcinoma

2010 ◽  
Vol 31 (11) ◽  
pp. 1487-1494 ◽  
Author(s):  
Yi Guo ◽  
Jia-xin Chen ◽  
Shu Yang ◽  
Xu-ping Fu ◽  
Zheng Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nasopharyngeal Carcinoma ◽  
Reference Genes ◽  
Gene Expression Study ◽  
Expression Study ◽  
Selection Of

scholarly journals Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas Disease

2021 ◽  
Vol 9 ◽  
Author(s):  
Axelle Marchant ◽  
Florence Mougel ◽  
Emmanuelle Jacquin-Joly ◽  
Carlos E. Almeida ◽  
Denis Blanchet ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chagas Disease ◽  
Large Set ◽  
Gene Expression Study ◽  
Expression Study ◽  
Clear Cut ◽  
Chemosensory Gene ◽  
Chemosensory Perception ◽  
Differential Gene ◽  
Rhodnius Robustus

Two closely related species, Rhodnius prolixus and Rhodnius robustus, are the vectors of Trypanosoma cruzi, which is the causative agent of Chagas disease, but clearly exhibit clear-cut differences in their ecological behavior. R. prolixus is considered as a domiciliated species, whereas R. robustus only sporadically visits human houses in Amazonia. We performed a chemosensory gene expression study via RNA-sequencing (RNA-seq) for the two species and also included a laboratory introgressed R. robustus strain. We built an assembled transcriptome for each sample and for both sexes and compiled all in a reference transcriptome for a differential gene expression study. Because the genes specifically expressed in one condition and not expressed in another may also reflect differences in the adaptation of organisms, a comparative study of the presence/absence of transcripts was also performed for the chemosensory transcripts, namely chemosensory proteins (CSPs), odorant-binding proteins (OBPs), odorant receptors (ORs), gustatory receptors (GRs), and ionotropic receptors (IRs), as well as takeout (TO) transcripts because TO proteins have been proposed to be associated with chemosensory perception in both olfactory and taste systems. In this study, 12 novel TO transcripts from the R. prolixus genome were annotated. Among the 199 transcripts, out of interest, annotated in this study, 93% were conserved between R. prolixus and the sylvatic R. robustus. Moreover, 10 transcripts out of interest were specifically expressed in one sex and absent in another. Three chemosensory transcripts were found to be expressed only in the reared R. prolixus (CSP19, OBP9, and OR89) and only one in sylvatic R. robustus (OR22). A large set of transcripts were found to be differentially expressed (DE) between males and females (1,630), with a majority of them (83%) overexpressed in males. Between environmental conditions, 8,596 transcripts were DE, with most (67%) overexpressed in the sylvatic R. robustus samples, including 17 chemosensory transcripts (4 CSPs, 1 OBP, 5 ORs, 1 GR, 4 IR, and 2 TO), but 4 genes (OBP19, OR13, OR40, and OR79) were overexpressed in the reared samples.

Validation of reference genes in Penicillium echinulatum to enable gene expression study using real-time quantitative RT-PCR

2014 ◽  
Vol 60 (3) ◽  
pp. 231-236 ◽  
Author(s):  
Denise Zampieri ◽  
Luísa C. Nora ◽  
Vanessa Basso ◽  
Marli Camassola ◽  
Aldo J. P. Dillon
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Reference Genes ◽  
Gene Expression Study ◽  
Rt Pcr ◽  
Expression Study ◽  
Penicillium Echinulatum
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