scholarly journals Selection of reliable reference genes for gene expression study in nasopharyngeal carcinoma

2010 ◽  
Vol 31 (11) ◽  
pp. 1487-1494 ◽  
Author(s):  
Yi Guo ◽  
Jia-xin Chen ◽  
Shu Yang ◽  
Xu-ping Fu ◽  
Zheng Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nasopharyngeal Carcinoma ◽  
Reference Genes ◽  
Gene Expression Study ◽  
Expression Study ◽  
Selection Of

scholarly journals Critical selection of reliable reference genes for gene expression study in the HepaRG cell line

2011 ◽  
Vol 81 (10) ◽  
pp. 1255-1261 ◽  
Author(s):  
Liesbeth Ceelen ◽  
Ward De Spiegelaere ◽  
Michael David ◽  
Jurgen De Craene ◽  
Mathieu Vinken ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Reference Genes ◽  
Gene Expression Study ◽  
Expression Study ◽  
Heparg Cell ◽  
Heparg Cell Line ◽  
Selection Of

Validation of reference genes in Penicillium echinulatum to enable gene expression study using real-time quantitative RT-PCR

2014 ◽  
Vol 60 (3) ◽  
pp. 231-236 ◽  
Author(s):  
Denise Zampieri ◽  
Luísa C. Nora ◽  
Vanessa Basso ◽  
Marli Camassola ◽  
Aldo J. P. Dillon
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Reference Genes ◽  
Gene Expression Study ◽  
Rt Pcr ◽  
Expression Study ◽  
Penicillium Echinulatum

Comparative gene expression study in WM164 melanoma cells treated with dimethylacrylshikonin.

2017 ◽  
Author(s):  
N Kretschmer ◽  
A Deutsch ◽  
B Rinner ◽  
M Scheideler ◽  
R Bauer
Keyword(s):  
Gene Expression ◽  
Melanoma Cells ◽  
Gene Expression Study ◽  
Expression Study

A gene expression study of the glutamate-nitric oxide pathway in schizophrenia

2018 ◽  
Author(s):  
Makoto Kinoshita ◽  
Florian Freudenberg ◽  
Esin Candemir ◽  
Sarah Kittel-Schneider
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Gene Expression Study ◽  
Expression Study ◽  
Nitric Oxide Pathway

scholarly journals Spatio-temporal selection of reference genes in the two congeneric species of Glycyrrhiza

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuping Li ◽  
Xiaoju Liang ◽  
Xuguo Zhou ◽  
Yu An ◽  
Ming Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Developmental Stage ◽  
Reference Genes ◽  
Developmental Stages ◽  
Individual Factors ◽  
Congeneric Species ◽  
Spatio Temporal ◽  
The Stability ◽  
Different Tissues ◽  
Selection Of

AbstractGlycyrrhiza, a genus of perennial medicinal herbs, has been traditionally used to treat human diseases, including respiratory disorders. Functional analysis of genes involved in the synthesis, accumulation, and degradation of bioactive compounds in these medicinal plants requires accurate measurement of their expression profiles. Reverse transcription quantitative real-time PCR (RT-qPCR) is a primary tool, which requires stably expressed reference genes to serve as the internal references to normalize the target gene expression. In this study, the stability of 14 candidate reference genes from the two congeneric species G. uralensis and G. inflata, including ACT, CAC, CYP, DNAJ, DREB, EF1, RAN, TIF1, TUB, UBC2, ABCC2, COPS3, CS, R3HDM2, were evaluated across different tissues and throughout various developmental stages. More importantly, we investigated the impact of interactions between tissue and developmental stage on the performance of candidate reference genes. Four algorithms, including geNorm, NormFinder, BestKeeper, and Delta Ct, were used to analyze the expression stability and RefFinder, a comprehensive software, provided the final recommendation. Based on previous research and our preliminary data, we hypothesized that internal references for spatio-temporal gene expression are different from the reference genes suited for individual factors. In G. uralensis, the top three most stable reference genes across different tissues were R3HDM2, CAC and TUB, while CAC, CYP and ABCC2 were most suited for different developmental stages. CAC is the only candidate recommended for both biotic factors, which is reflected in the stability ranking for the spatio (tissue)-temporal (developmental stage) interactions (CAC, R3HDM2 and DNAJ). Similarly, in G. inflata, COPS3, R3HDM2 and DREB were selected for tissues, while RAN, COPS3 and CS were recommended for developmental stages. For the tissue-developmental stage interactions, COPS3, DREB and ABCC2 were the most suited reference genes. In both species, only one of the top three candidates was shared between the individual factors and their interactions, specifically, CAC in G. uralensis and COPS3 in G. inflata, which supports our overarching hypothesis. In summary, spatio-temporal selection of reference genes not only lays the foundation for functional genomics research in Glycyrrhiza, but also facilitates these traditional medicinal herbs to reach/maximize their pharmaceutical potential.

scholarly journals Identification of Novel GH-Regulated Pathway of Lipid Metabolism in Adipose Tissue: A Gene Expression Study in Hypopituitary Men

2011 ◽  
Vol 96 (7) ◽  
pp. E1188-E1196 ◽  
Author(s):  
Jing Ting Zhao ◽  
Mark J. Cowley ◽  
Paul Lee ◽  
Vita Birzniece ◽  
Warren Kaplan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Gene Expression Study ◽  
Expression Study
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