Effect of Temperature, Carbon Source, Yeast Extract, and pH on Pullulan Production by Aureobasidium pullulans

2009 ◽  
Author(s):  
Kuan-Chen Cheng ◽  
Ali Demirci ◽  
Jeffrey Catchmark
1996 ◽  
Vol 57-58 (1) ◽  
pp. 827-835 ◽  
Author(s):  
Kirit D. Chapatwala ◽  
G. R. V. Babu ◽  
Onguri K. Vijaya ◽  
E. Armstead ◽  
A. V. Palumbo ◽  
...  

2010 ◽  
Vol 65 (9-10) ◽  
pp. 594-598 ◽  
Author(s):  
Wei Wu ◽  
Mingbo Lu ◽  
Longjiang Yu

The wild strain and two astaxanthin-overproducing mutant strains, W618 and GNG274, of Xanthophyllomyces dendrorhous were analyzed in order to assess their ability to grow and synthesize astaxanthin in a minimal medium containing (per liter): 2 g KH2PO4, 0.5 g MgSO4, 2 g KNO3, and 1 g yeast extract, and supplemented with citrus residues isolates as a carbon source (citrus medium). The selected strain W618 was evaluated under various contents of citrus juice. At the content of 20% (v/v), the highest astaxanthin production reached 22.63 mg L-1, which was two-fold more than that observed in yeast malt medium. Addition of 8% (v/v) n-hexadecane to the citrus medium was found to be optimal, increasing the astaxanthin yield by 21.7%.


2013 ◽  
Vol 641-642 ◽  
pp. 206-210 ◽  
Author(s):  
Lei Huang ◽  
Jing Xie ◽  
Xiao Feng Shi ◽  
Jing Yan Lian

A hydrocarbon-degrading strain ZRS was isolated from petroleum-contaminated soils sampled from Xinjiang oil field and identified as Acinetobacter beijerinckii, which could use diesel oil as solo carbon source. The optimal temperature and pH for strain utilizing ethanol was 25°C and 7.2; the optimal concentration of ethanol and the biomass concentration was 4.5% and 109 CFU/mL, respectively. Inoculated to artificial seawater which added (NH4)2SO4 2.64g/L、Na2HPO4 1.5 g/L and yeast extract 13 mg/L after 7 days of culture at temperature 25°C, the rate of degradation was 73.9%.


2015 ◽  
Vol 45 (9) ◽  
pp. 1707-1712 ◽  
Author(s):  
Luiz Jardel Visioli ◽  
Eliana Albornoz Alves ◽  
Aline Trindade ◽  
Raquel Cristine Kuhn ◽  
Marcio Schwaab ◽  
...  

<p>In this research it was evaluated the production of biobutanol by<bold> Clostridium beijerinckii</bold>NRRL B-592 using sweet sorghum juice as carbon source. Operational variables, like pH and initial inoculum size, as well as supplementation of industrial media with yeast extract and tryptone, were evaluated. The maximum butanol obtained was 2.12g kg<sup>-1</sup> using 12.5% of inoculum size, 0.05g 100mL<sup>-1</sup> of tryptone and 0.1g 100mL<sup>-1</sup> of yeast extract and initial pH of 5.5. The main contribution of this research was to show a systematic procedure for development of a low cost industrial media for biobutanol production from sweet sorghum.</p>


2020 ◽  
pp. 0958305X2090806
Author(s):  
Ravichandran Viveka ◽  
Sunita Varjani ◽  
Nakkeeran Ekambaram

The interest in pullulan produced from industrial wastes has substantially increased due to its industrial applications. The major constraint in pullulan production is expensive and low yield. In this study, attempts were made on the evaluation of media components for maximum pullulan production from Aureobasidium pullulans MTCC 1991 using cassava waste in solid-state fermentation. A statistical approach was scrutinized to evaluate the effects of yeast extract, K2HPO4, (NH4)2SO4, MgSO4⋅7H2O, and NaCl on pullulan production. The optimal conditions eliciting the maximum pullulan yield (6.45 g pullulan/L of culture broth) were found to be 0.6 g/L yeast extract, 3 g/L K2HPO4, 0.2 g/L (NH4)2SO4, 0.3 g/L MgSO4⋅7H2O, and 1 g/L NaCl. The produced pullulan was extracted using the solvent precipitation method and characterized by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy spectroscopic analysis that confirmed pullulan with linear α (1–6) linkage. The results revealed that cassava waste could be used as an effective carbon source for the production of pullulan.


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