Effect of carbon source on polysaccharide production by alginate-entrapped Aureobasidium pullulans ATCC 42023 cells

2011 ◽  
Vol 51 (6) ◽  
pp. 673-677 ◽  
Author(s):  
Thomas P. West
1968 ◽  
Vol 14 (10) ◽  
pp. 1063-1068 ◽  
Author(s):  
James L. Goatley

Alternaria solani produced two homogeneous exocellular polysaccharide fractions when grown in shake culture with fructose as the carbon source and the NH4NO3 concentration of the medium 1.7 g/l. Chromatographic techniques indicated that fraction I contained glucose, galactose, and glucosamine in a 2.4:1:1 ratio whereas fraction II contained glucose, galactose, and mannose in a 6.4:1:1.2 ratio. Fraction I was found at levels seven times greater than II. Total polysaccharide produced was 1.8% of the dry weight of the mycelium and 1.2% of the available carbohydrate.Growth and polysaccharide production were greatest with fructose as the carbon source. Decreasing levels of growth occurred with mannitol, glucose, and sucrose, respectively. Exocellular polysaccharides were not detected with mannitol as carbon source. At 10 days, growth on fructose is maximum at NH4NO3 concentrations of 1.5 to 2.5 g/l, but exocellular polysaccharide production is maximum at 0.8 g/l. When produced on a medium of 2.0 g/l NH4NO3 the nitrogen content of I is double that when the medium is 0.8 g/l NH4NO3. In addition the ratio of I to II is about four times greater.


1986 ◽  
Vol 32 (9) ◽  
pp. 756-758 ◽  
Author(s):  
Lucia Costamagna ◽  
Iolanda Rosi ◽  
Isabella Garuccio ◽  
Oreste Arrigoni

One hundred and eighty strains of yeasts belonging to 17 genus and 53 species were screened for their ability to grow on ascorbic acid and iso-ascorbic acid as the sole carbon source. Most of the tested strains (157) were unable to grow on either compound. Strains of seven species of the genus Cryptococcus, of two Candida species, of Filobasidiella neoformans, Trichosporon cutaneum, Lipomyces starkeyi, Hansenula capsulata, and one strain of Aureobasidium pullulans were able to grow on ascorbic as well as on iso-ascorbic acid. Conversely, four strains of Aureobasidium pullulans, Candida blankii, and Cryptococcus dimennae could use only ascorbic acid for growth.


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