Cross-linkable and water-soluble phospholipid polymer as artificial extracellular matrix

2014 ◽  
Vol 1 (3) ◽  
pp. 163-174
Author(s):  
Eri Maeta ◽  
Kazuhiko Ishihara
Keyword(s):  
Extracellular Matrix ◽  
Water Soluble ◽  
Phospholipid Polymer ◽  
Artificial Extracellular Matrix

scholarly journals Electrospun Nanofibers of Natural and Synthetic Polymers as Artificial Extracellular Matrix for Tissue Engineering

Nanomaterials ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 21
Author(s):  
Mina Keshvardoostchokami ◽  
Sara Seidelin Majidi ◽  
Peipei Huo ◽  
Rajan Ramachandran ◽  
Menglin Chen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Polymer Composite ◽  
Electrospun Nanofibers ◽  
Synthetic Polymer ◽  
Inorganic Materials ◽  
Host Cells ◽  
Natural Polymer ◽  
Reinforced Polymers ◽  
Nanofibrous Scaffolds ◽  
Artificial Extracellular Matrix

Many types of polymer nanofibers have been introduced as artificial extracellular matrices. Their controllable properties, such as wettability, surface charge, transparency, elasticity, porosity and surface to volume proportion, have attracted much attention. Moreover, functionalizing polymers with other bioactive components could enable the engineering of microenvironments to host cells for regenerative medical applications. In the current brief review, we focus on the most recently cited electrospun nanofibrous polymeric scaffolds and divide them into five main categories: natural polymer-natural polymer composite, natural polymer-synthetic polymer composite, synthetic polymer-synthetic polymer composite, crosslinked polymers and reinforced polymers with inorganic materials. Then, we focus on their physiochemical, biological and mechanical features and discussed the capability and efficiency of the nanofibrous scaffolds to function as the extracellular matrix to support cellular function.

Promotion of Angiogenesis by an Artificial Extracellular Matrix Protein Containing the Laminin-1-Derived IKVAV Sequence

2009 ◽  
Vol 20 (9) ◽  
pp. 1759-1764 ◽  
Author(s):  
Makiko Nakamura ◽  
Kumiko Yamaguchi ◽  
Masayasu Mie ◽  
Makoto Nakamura ◽  
Keiichi Akita ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Artificial Extracellular Matrix ◽  
Laminin 1

Transformable Nanomaterials as an Artificial Extracellular Matrix for Inhibiting Tumor Invasion and Metastasis

ACS Nano ◽  
2017 ◽  
Vol 11 (4) ◽  
pp. 4086-4096 ◽  
Author(s):  
Xiao-Xue Hu ◽  
Ping-Ping He ◽  
Guo-Bin Qi ◽  
Yu-Juan Gao ◽  
Yao-Xin Lin ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Tumor Invasion ◽  
Invasion And Metastasis ◽  
Artificial Extracellular Matrix

Construction of Hybrid Cell Spheroids Using Cell-Sized Cross-Linked Nanogel Microspheres as an Artificial Extracellular Matrix

2021 ◽  
Author(s):  
Shunya Hayashi ◽  
Yoshihiro Sasaki ◽  
Hirotaka Kubo ◽  
Shin-ichi Sawada ◽  
Naoya Kinoshita ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Hybrid Cell ◽  
Cell Spheroids ◽  
Artificial Extracellular Matrix

Bioinspired and Multifunctional Phospholipid Polymer Nanoparticles

2016 ◽  
Vol 102 ◽  
pp. 3-11
Author(s):  
Kazuhiko Ishihara ◽  
Wei Xin Chen ◽  
Yuuki Inoue
Keyword(s):  
Acid Group ◽  
Covalent Bonding ◽  
Water Soluble ◽  
Polymer Nanoparticles ◽  
Butyl Methacrylate ◽  
Carboxylic Acid Group ◽  
Cleavage Reaction ◽  
Phospholipid Polymer ◽  
Biodegradable Poly ◽  
Immobilized Proteins

Photoreactive and cytocompatible polymer nanoparticles for immobilizing and photoinduced releasing proteins were prepared. A water-soluble and amphiphilic phospholipid polymer, poly (2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-4-(4-(1-methacryloyloxyethyl)-2-methoxy-5-nitrophenoxy) butyric acid (PL)) (PMB-PL) was synthesized. The PMB-PL underwent a cleavage reaction at the PL unit by photoirradiation at a wavelength of 365 nm. Additionally, the PMB-PL took polymer aggregate in aqueous medium and was used to modify the surface of biodegradable poly (L-lactic acid) (PLA) nanoparticle as an emulsifier. The morphology of the PMB-PL/PLA nanoparticle was spherical and approximately 130 nm in diameter. The carboxylic acid group in the PL unit could be used for immobilization of proteins by covalent bonding. The bound proteins were released by a photoinduced cleavage reaction. Within 60 sec, up to 90% of the immobilized proteins were released by photoirradiation and activity of the protein released in the medium was maintained as well as that the original proteins before immobilization. Octa-arginine (R8) could promote internalization of the protein/PLA/PMB-PL nanoparticles into cells when the R8 was co-immobilized on the nanoparticles. After that, photoirradiation induced protein release from the nanoparticles and proteins distributed more evenly inside cells. From these results, we concluded that PMB-PL/PLA nanoparticles have the potential to be used as smart carriers to deliver proteins to biological systems, such as the inside of living cells.

A fusion protein N-cadherin-Fc as an artificial extracellular matrix surface for maintenance of stem cell features

Biomaterials ◽  
2010 ◽  
Vol 31 (20) ◽  
pp. 5287-5296 ◽  
Author(s):  
Xiao-Shan Yue ◽  
Yuta Murakami ◽  
Toshiyuki Tamai ◽  
Masato Nagaoka ◽  
Chong-Su Cho ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Fusion Protein ◽  
Matrix Surface ◽  
Artificial Extracellular Matrix

Cell adhesion on an artificial extracellular matrix using aptamer-functionalized PEG hydrogels

Biomaterials ◽  
2012 ◽  
Vol 33 (5) ◽  
pp. 1353-1362 ◽  
Author(s):  
Niancao Chen ◽  
Zhaoyang Zhang ◽  
Boonchoy Soontornworajit ◽  
Jing Zhou ◽  
Yong Wang
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Peg Hydrogels ◽  
Artificial Extracellular Matrix

Injection mixed drug intratumoral to produce ROS and creating a coagulum as cancer therapeutics drug depot.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e15635-e15635
Author(s):  
Jian Zhang ◽  
Qiang Fu ◽  
Bian Li ◽  
Yan Han ◽  
Dong Chen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Tumor Imaging ◽  
Cancer Therapeutics ◽  
Water Soluble ◽  
Control Group ◽  
Histological Structure ◽  
High Concentration ◽  
Long Time ◽  
Group 2 ◽  
Scanning Imaging

e15635 Background: To explore the nature of hydrogen peroxide at higher dosage for denature-aggregation of tumor with drugs through aggregation in denatured tumor as a coagulum for drug depot and prolong function of drug. Methods: (1). Preparation for two 5 ml of BLM-I131, one diluted with 0.12 ml NS and 0.2 ml (0.2mCi = 49.67µg BLM) for tumor injection in control group; one diluted with 0.12 ml of H2O2 (0.833 mg/ml) and 0.2 ml (0.2mCi = 49.67µg BLM) for tumor injection in experimental group. (2).Tumor imaging with BLM-I131 analyzed at different time points. (3). Radioactivity in tumor of mice analyzed under SPECT scanning imaging instrument at the 0, 5 h, 1 h, 2 h, 4 h, 8 h, 24 h, 48 h, 96 h, 120 h, 144 h and 168 h, activity of isotope I131 is representing the BLM retaining time in tumor. (4). Also, tumor sectioned and observed cellular and extracellular matrix changes of histological structure. Results: It was observed that BLM-I131 with ROS in tumors sustained for 168 hours while BLM-I131 with NS in tumors sustained for 8 hours only. Radioactivity of BLM-I131 in tumors with ROS reach at peak 1.5 hours and second peak at 20 hours by average to extend to 168 hours, while radioactivity of BLM-I131 in tumors with NS reached at peak in 0.5 hour, decreased to 30% in 1.5 hour quickly to background in 8 hours; We observed the extracellular matrix changes in experimental tumor while no changes in control tumor. Conclusions: A water soluble oxidant mixed with free drug can play a biological scissors role to chop tumor matrix, then it resulted in a denature tumor matrix into a coagulation for a drug depot, it showed drug of BLM-I131 sustained in tumor for a long time; while oxidant plays an important role to punch holes on cell membrane and resulted a high permeability for high concentration drug in each cancer cells.

Fabrication of BSA Loaded Poly(Caprolactone) (PCL) Microsphere Incorporated Chitosan Scaffolds for Tissues Engineering Application

2014 ◽  
Vol 695 ◽  
pp. 199-202
Author(s):  
Mad Jin Rashid ◽  
Lari Ali Reza ◽  
Naznin Sultana
Keyword(s):  
Extracellular Matrix ◽  
Engineering Application ◽  
Chitosan Solution ◽  
Living Cells ◽  
Porous Scaffolds ◽  
High Porosity ◽  
Chitosan Scaffolds ◽  
Artificial Extracellular Matrix ◽  
Poly Caprolactone ◽  
Suitable Environment

Scaffolds-based tissues engineering involves the combination of an artificial extracellular matrix (ECM), living cells, with high porosity and well connected pores that will provide suitable environment for cells. In this study, firstly, poly (caprolactone) (PCL)-based microspheres were synthesized and characterized. Bovine serum albumin (BSA) (0.04% w/v) was added into the microspheres produced from 5% (w/v) PCL concentration. BSA loaded microspheres were then incorporated into chitosan solution to fabricate porous scaffolds. The scaffolds were then characterized using different techniques.

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