scholarly journals Protective Effect of Pasteurella multocida Cell-Free Antigen and Toxoid against Challenge with Toxigenic Strains of Pasteurella multocida in Mice

2003 ◽  
Vol 65 (6) ◽  
pp. 737-740 ◽  
Author(s):  
Chikara UCHIDA ◽  
Yoshihiro KIMURA ◽  
Sei KUBOTA ◽  
Osamu SASAKI
Keyword(s):  
Protective Effect ◽  
Pasteurella Multocida ◽  
Toxigenic Strains ◽  
Free Antigen

scholarly journals Use of ELISA to Detect Toxigenic Pasteurella Multocida in Atrophic Rhinitis in Swine

1992 ◽  
Vol 4 (4) ◽  
pp. 419-422 ◽  
Author(s):  
Terry L. Bowersock ◽  
Tom Hooper ◽  
Ronald Pottenger
Keyword(s):  
Tissue Culture ◽  
Pasteurella Multocida ◽  
Atrophic Rhinitis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cytotoxicity Assays ◽  
Highly Sensitive ◽  
Nasal Secretions ◽  
Toxigenic Strains ◽  
Swine Herds ◽  
Toxigenic Pasteurella Multocida

The use of an enzyme-linked immunosorbent assay (ELISA) as a means of detecting dermone-crotoxin-producing strains of Pasteurella multocida was investigated. The assay was evaluated as a means to identify toxigenic P. multocida isolates recovered from nasal secretions of swine with atrophic rhinitis. The sensitivity and specificity of the ELISA for detecting dermonecrotoxin-producing P. multocida strains were compared to those of mouse-inoculation and cytotoxicity assays. The ELISA was highly sensitive and more specific than animal inoculation or tissue culture assay and is thus a more effective method for screening swine herds for the presence of toxigenic strains of P. multocida. The ELISA is a rapid, effective, economical way to identify toxigenic P. multocida isolates.

scholarly journals Protective effect of intranasal peste des petits ruminants virus and bacterin vaccinations: Clinical, hematological, serological, and serum oxidative stress changes in challenged goats

2019 ◽  
Vol 12 (7) ◽  
pp. 945-950
Author(s):  
Theophilus Aghogho Jarikre ◽  
Jeremiah Olalekan Taiwo ◽  
Benjamin Obukowho Emikpe ◽  
Stephen Owarioro Akpavie
Keyword(s):  
Protective Effect ◽  
Pasteurella Multocida ◽  
Glutathione Transferase ◽  
Clinical Signs ◽  
Vaccination Strategy ◽  
Peste Des Petits Ruminants ◽  
Mucosal Vaccination ◽  
Spectrophotometric Methods ◽  
Stress Changes ◽  
Hydrogen Peroxide Generation

Background and Aim: The current vaccination for peste des petits ruminants virus (PPRV) is stalled by myriad challenges and continuous endemicity of pneumonia due to fulminant bacterial complication in goats. The present study evaluated the protective effect of intranasal PPRV linage 1 and bacterine vaccinations. Materials and Methods: Twelve West African Dwarf (WAD) goats aged 6 months were randomly grouped and vaccinated within 2 weeks using a combination of PPRV lineage 1 vaccine (Nig/75), and bacterin from Mannheimia haemolytica (Mh) or Pasteurella multocida intranasally. The goats were observed for 3 weeks post-vaccination before comingled with a known infected WAD goat with apparent clinical signs of peste des petits ruminants and further observed clinically for 5 weeks post-infection (PI). Blood samples were taken for hematology while sera were assayed for antioxidants (glutathione peroxidase, glutathione transferase, and superoxide dismutase) activities and pro-oxidants (malondialdehyde content, reduced glutathione, hydrogen peroxide generation, and myeloperoxidase) using spectrophotometric methods. Data were subjected to parametric statistics at α=0.05 using GraphPad Prism version 21. Results: Clinically, there were pyrexia, oculonasal discharge, diarrhea, anemia, leukopenia, and increased pro-oxidants in the unvaccinated goats, while moderate neutrophilia and leukocytosis were observed in PPRV and bacterin vaccinated goats. Two unvaccinated goats were weak and euthanized at 13 and 28 days PI. The goats vaccinated with PPRV and Mh showed better response clinically and biochemically. Conclusion: The mucosal vaccination of goats with PPRV vaccine and bacterine will protect against exposure and culminate in the development of protective mucosal, humoral, and cell-mediated immune responses. This vaccination strategy will provide framework needed in the prevention and control of endemic caprine pneumonia in Nigeria.

scholarly journals Protective Effect of the Combined vaccine Prepared from Cell-Free-Antigen of Actinobacillus pleuropneumoniae Serotypes 1,2 and 5 in Pigs.

1995 ◽  
Vol 57 (6) ◽  
pp. 1125-1128 ◽  
Author(s):  
Eiji OISHI ◽  
Takashi KITAJIMA ◽  
Yoko KOYAMA ◽  
Toshiaki OHGITANI ◽  
Shigeji KATAYAMA ◽  
...  
Keyword(s):  
Protective Effect ◽  
Actinobacillus Pleuropneumoniae ◽  
Combined Vaccine ◽  
Free Antigen

scholarly journals Immune Protective Efficacy of China’s Traditional Inactivated and Attenuated Vaccines against the Prevalent Strains of Pasteurella multocida in Mice

Vaccines ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1155
Author(s):  
Li-Jun Guan ◽  
Ji-Jian Song ◽  
Yun Xue ◽  
Xia Ai ◽  
Zhi-Jun Liu ◽  
...  
Keyword(s):  
Mouse Model ◽  
Protective Effect ◽  
Aluminum Hydroxide ◽  
Pasteurella Multocida ◽  
Protective Efficacy ◽  
Cross Protection ◽  
Immune Protection ◽  
Capsular Type ◽  
Protection Effect ◽  
Antibody Titers

Capsular type A and D strains of Pasteurella multocida are the main epidemic serogroups in pigs in China. In this study, we preliminarily evaluated the immune protective efficacy of the two traditional vaccines, an inactivated C44-1 aluminum-hydroxide-gel-adjuvanted (Alh–C44-1) vaccine and a live EO630 vaccine, against currently circulating strains of P. multocida in a mouse model. Mice immunized twice with conventional vaccines generated higher antibody titers, and significantly higher levels of IgG were observed in the mice inoculated with the inactivated Alh–C44-1 vaccine on day 35 (p < 0.05) than those with the live EO630 vaccine. The mice immune protection test showed that the vaccination groups had a 57% or 71% protection effect against the serogroup B strain, but had no protective effect against epidemic strains. In conclusion, our study found that the widely used traditional P. multocida vaccines in China provide good protection against homologous strains, but could not provide cross-protection against heterologous strains in a mouse model.

scholarly journals Protective Effect by Cell-Free Antigen Obtained from Culture Supernatant of Phase I Bordetella bronchiseptica.

1992 ◽  
Vol 54 (1) ◽  
pp. 37-42
Author(s):  
Toshiaki OHGITANI ◽  
Chikara UCHIDA ◽  
Tatsuji OKABE ◽  
Norimasa SASAKI
Keyword(s):  
Phase I ◽  
Protective Effect ◽  
Culture Supernatant ◽  
Bordetella Bronchiseptica ◽  
Free Antigen

Protective effect of chitooligosaccharides on hydrogen peroxide-induced PC-12 cells death by inhibition of oxidative damage

2010 ◽  
Vol 34 (8) ◽  
pp. S27-S27
Author(s):  
Xueling Dai ◽  
Ping Chang ◽  
Ke Xu ◽  
Changjun Lin ◽  
Hanchang Huang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Oxidative Damage ◽  
Protective Effect ◽  
Pc 12 Cells ◽  
Cells Death
Sign in / Sign up

Export Citation Format

Share Document