Histological Studies of Putrid Abdominal Adhesions as Tool in the Forensic Medical Examination

10.12737/9083 ◽  
2015 ◽  
Vol 22 (1) ◽  
pp. 79-82
Author(s):  
Калашникова ◽  
S. Kalashnikova ◽  
Барканов ◽  
V. Barkanov ◽  
Горячев ◽  
...  
Keyword(s):  
Soft Tissues ◽  
Muscle Fibers ◽  
Muscle Cells ◽  
Differential Staining ◽  
Cellular Composition ◽  
Abdominal Adhesions ◽  
Tissue Adhesions ◽  
Reticular Fibers ◽  
Traumatic Origin ◽  
Hematoxylin And Eosin

Histological examination of putrid adhesions is informative to determine the time of death and the etiology of origin of adhesions. Differential staining of adhesions with hematoxylin and eosin, trichrome by Masson, Zerbino with prescription death up to 7 days allows to define the appearance of the cellular composition, staining of muscle fibers on Zerbino and collagen and reticular fibers. In case of death from 7 to 10 days, the loss of the cellular composition in the color with hematoxylin and eosin is determined in tissue adhesions. This preserves the color of the muscle cells on Zerbino and staining of collagen and reticular fibers in the color of trigram by Masson. In the period from 10 to 14 days after the death, adhesions additionally lose the ability to staining of muscle cells on Zerbino. In case of death of more than 14 days, reticular fibers don’t turn spinach. Color is kept only for the collagen fibers in the color of trigram by Masson. In the case of traumatic and non-traumatic origin of putrid modified adhesions on histological sections of adhesions in color according to Perls, the signs of hemorrhage in the area of adhesions in the presence of compounds of trivalent iron are identified. In case of accumulation of the dye in the blue-green color in the form of granules in the soldering and soft tissues of the insertion or impregnation of the dye of the connective tissue and putrid modified muscle fibers, it is possible to diagnose traumatic origin of putrid modified adhesions, and in the absence of positive reactions – their non-traumatic origin.

scholarly journals In vivo genome editing in mouse restores dystrophin expression in Duchenne muscular dystrophy patient muscle fibers

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Menglong Chen ◽  
Hui Shi ◽  
Shixue Gou ◽  
Xiaomin Wang ◽  
Lei Li ◽  
...  
Keyword(s):  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Mouse Model ◽  
Genome Editing ◽  
Large Scale ◽  
Gene Editing ◽  
High Efficiency ◽  
Muscle Fibers ◽  
Muscle Cells

Abstract Background Mutations in the DMD gene encoding dystrophin—a critical structural element in muscle cells—cause Duchenne muscular dystrophy (DMD), which is the most common fatal genetic disease. Clustered regularly interspaced short palindromic repeat (CRISPR)-mediated gene editing is a promising strategy for permanently curing DMD. Methods In this study, we developed a novel strategy for reframing DMD mutations via CRISPR-mediated large-scale excision of exons 46–54. We compared this approach with other DMD rescue strategies by using DMD patient-derived primary muscle-derived stem cells (DMD-MDSCs). Furthermore, a patient-derived xenograft (PDX) DMD mouse model was established by transplanting DMD-MDSCs into immunodeficient mice. CRISPR gene editing components were intramuscularly delivered into the mouse model by adeno-associated virus vectors. Results Results demonstrated that the large-scale excision of mutant DMD exons showed high efficiency in restoring dystrophin protein expression. We also confirmed that CRISPR from Prevotella and Francisella 1(Cas12a)-mediated genome editing could correct DMD mutation with the same efficiency as CRISPR-associated protein 9 (Cas9). In addition, more than 10% human DMD muscle fibers expressed dystrophin in the PDX DMD mouse model after treated by the large-scale excision strategies. The restored dystrophin in vivo was functional as demonstrated by the expression of the dystrophin glycoprotein complex member β-dystroglycan. Conclusions We demonstrated that the clinically relevant CRISPR/Cas9 could restore dystrophin in human muscle cells in vivo in the PDX DMD mouse model. This study demonstrated an approach for the application of gene therapy to other genetic diseases.

scholarly journals Мікроскопічні зміни в нирках і міокарді курей за інфекційного ларинготрахеїту

2013 ◽  
pp. 54-55
Author(s):  
Б. В. Борисевич ◽  
В. В. Лісова ◽  
М. С. Криштоп
Keyword(s):  
Blood Vessels ◽  
Muscle Fibers ◽  
Muscle Cells ◽  
Degenerative Changes ◽  
Histological Studies ◽  
Infectious Laryngotracheitis ◽  
Circulatory Disorders

Представлено результати гістологічних дослі-джень нирок і міокарду курей, які загинули від інфек-ційного ларинготрахеїту. Встановлено, що в нирках реєструються розлади кровообігу в вигляді розши-рення та переповнення кров’ю кровоносних судин строми органа, екстракапілярний серозний гломеру-лонефрит і дистрофічні зміни та руйнування епіте-лію канальців. У міокарді провідною патологією була зерниста дистрофія м’язових клітин. Окрім того реєструвалися фрагментація та дезорієнтація м’язових волокон. У епікарді та ендокарді мікроско-пічних змін не було. The results of histological studies of kidneys and myocardium of chickens that died of infectious laryngotracheitis have been shown. It was found that circulatory disorders in the form of expansion and overflow of blood with blood vessels of body stroma, extra serosal capillary glomerulonephritis and degenerative changes and destruction of epithelial tubules are registered in the kidneys. In the myocardium the leading pathology was granular dystrophy of muscle cells. In addition, fragmentation and disorientation of muscle fibers was registered. In the epicardium and endocardium there were no microscopic changes.

scholarly journals Morphological changes in distant muscle fibers following thermal injury in Wistar rats

2010 ◽  
Vol 25 (6) ◽  
pp. 525-528 ◽  
Author(s):  
Flavia de Oliveira ◽  
Luci Rezende Bevilacqua ◽  
Carlos Alberto Anaruma ◽  
Silvia de Campos Boldrini ◽  
Edson Aparecido Liberti
Keyword(s):  
Body Surface ◽  
Wistar Rats ◽  
Thermal Injury ◽  
Morphological Changes ◽  
Muscle Fibers ◽  
The Body ◽  
Medial Gastrocnemius ◽  
Control Group ◽  
Hematoxylin And Eosin ◽  
Gastrocnemius Muscles

PURPOSE: Thermal injury causes catabolic processes as the body attempts to repair the damaged area. This study evaluated the effects of a scald injury on the morphology of muscle fibers belonging to a muscle distant from the lesion. METHODS: Thirty Wistar rats were divided into control (C) and scalded (S) groups. Group S was scalded over 45% of the body surface, standardized by body weight. Rats in both groups were euthanized at four, seven and 14 days following the injury. The middle portions of the medial gastrocnemius muscles were sectioned, stained with hematoxylin and eosin and Picrosirius, and submitted to histological analysis. RESULTS: Control group sections exhibited equidistantly distributed polygonal muscle fibers with peripheral nuclei, characteristic of normal muscle. The injured group sections did not consistently show these characteristics; many fibers in these sections exhibited a rounded contour, variable stain intensities, and greater interfiber distances. A substantially increased amount of connective tissue was also observed on the injured group sections. CONCLUSION: This experimental model found a morphological change in muscle distant from the site of thermal injury covering 45% of the body surface.

Glucose transporter expression in human skeletal muscle fibers

2000 ◽  
Vol 279 (3) ◽  
pp. E529-E538 ◽  
Author(s):  
M. Gaster ◽  
A. Handberg ◽  
H. Beck-Nielsen ◽  
H. D. Schrøder
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Glucose Transporter ◽  
Muscle Fibers ◽  
Muscle Cells ◽  
Glut 1 ◽  
Adult Muscle ◽  
Glut 4 ◽  
Skeletal Muscle Fibers ◽  
Transporter Expression

The present study was initiated to investigate GLUT-1 through -5 expression in developing and mature human skeletal muscle. To bypass the problems inherent in techniques using tissue homogenates, we applied an immunocytochemical approach, employing the sensitive enhanced tyramide signal amplification (TSA) technique to detect the localization of glucose transporter expression in human skeletal muscle. We found expression of GLUT-1, GLUT-3, and GLUT-4 in developing human muscle fibers showing a distinct expression pattern. 1) GLUT-1 is expressed in human skeletal muscle cells during gestation, but its expression is markedly reduced around birth and is further reduced to undetectable levels within the first year of life; 2) GLUT-3 protein expression appears at 18 wk of gestation and disappears after birth; and 3) GLUT-4 protein is diffusely expressed in muscle cells throughout gestation, whereas after birth, the characteristic subcellular localization is as seen in adult muscle fibers. Our results show that GLUT-1, GLUT-3, and GLUT-4 seem to be of importance during muscle fiber growth and development. GLUT-5 protein was undetectable in fetal and adult skeletal muscle fibers. In adult muscle fibers, only GLUT-4 was expressed at significant levels. GLUT-1 immunoreactivity was below the detection limit in muscle fibers, indicating that this glucose transporter is of minor importance for muscle glucose supply. Thus we hypothesize that GLUT-4 also mediates basal glucose transport in muscle fibers, possibly through constant exposure to tonal contraction and basal insulin levels.

scholarly journals HISTOLOGY OF THE SPLEEN OF INDIGENOUS DOG (CANIS FAMILIARIS) OF BANGLADESH

2012 ◽  
Vol 3 (1) ◽  
pp. 59-61 ◽  
Author(s):  
SK Das ◽  
MS Alam ◽  
MN Islam ◽  
MA Awal
Keyword(s):  
Smooth Muscle ◽  
Histological Study ◽  
Muscle Fibers ◽  
White Pulp ◽  
Reticular Fibers ◽  
Internal Structures ◽  
Different Shapes ◽  
Disease Free ◽  
Red Pulp ◽  
Apparently Healthy

The present research was conducted in the Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh from July to December 2003 with a view to study the detail histology of the spleen of indigenous dog of Bangladesh. Tissues were collected from the different parts of the spleen from the dogs killed after proper euthanasia. The adult experimental dogs were apparently healthy and disease free. The tissues were passed through different stains (Van Gieson's and Verhoeffs, Bielschowsky's Silver and Potassium Ferrocyanide stains) for detail histological study. The present study revealed that the spleen was encircled by capsule and trabeculae with the internal structures of white pulp, red pulp and sinusoids that are nearly similar as found in cattle. The capsule was composed of collagen, elastic, reticular and smooth muscle fibers. The trabeculae originated from the hilus that usually contained major vessels but was poor in smooth muscle fibers. The white pulp had a fine meshwork of reticular connective tissue containing mainly lymphocytes of various sizes. Meshworks of fine reticular fibers were observed throughout the red pulp in the present study. Sinusoids of the spleen of indigenous dog were of different shapes and sizes and widely distributed within the red pulp and had both arterial and venous communications.

scholarly journals Bacterial Distribution in Teeth with Pulp Necrosis and Apical Granuloma

2013 ◽  
Vol 2 (2) ◽  
pp. 86-91 ◽  
Author(s):  
Tiago Novaes Pinheiro ◽  
Alberto Consolaro ◽  
Francisco Carlos Ribeiro
Keyword(s):  
Root Canal ◽  
Bacterial Infections ◽  
Soft Tissues ◽  
Bacterial Biofilms ◽  
Gram Negative Bacteria ◽  
Apical Surface ◽  
Canal System ◽  
Microscopic Evaluation ◽  
Hematoxylin And Eosin ◽  
Root Canal System

ABSTRACT Aim To evaluate the distribution of bacteria in teeth with pulp necrosis and apical granuloma. Materials and methods Thirty-two extracted teeth with pulp necrosis and apical granulomas and 16 cases of isolated apical granulomas were sectioned and stained with Hematoxylin and Eosin (H&E) and Brown-Brenn staining for microscopic evaluation. The specimens were observed as to the location and distribution of bacteria involved in the process of pulp necrosis and their relationship with the periapical tooth structures and soft tissues. Results Stainable Gram-positive and Gram-negative bacteria were found in the entire root canal system. Bacterial biofilms were detected in 81.2% of cementum root canal, 46.8% in apical delta, 65.6% at the apical surface and 79.2% of the granulomas above all the specimens. Bacteria and bacterial biofilms were present in the entire root canal system of the infected teeth with apical granulomas. Conclusion Our findings suggest that bacterial infections can reach inaccessible areas to instrumentation. How to cite this article Ribeiro FC, Consolaro A, Pinheiro TN. Bacterial Distribution in Teeth with Pulp Necrosis and Apical Granuloma. Int J Experiment Dent Sci 2013;2(2):86-91.

scholarly journals Morphometric justification of osteosynthesis using implants coated with titanium and hafnium nitrides

2016 ◽  
Vol 97 (4) ◽  
pp. 585-591
Author(s):  
D E Tsyplakov ◽  
A E Izosimova ◽  
F V Shakirova ◽  
I F Akhtyamov ◽  
E B Gatina
Keyword(s):  
Stainless Steel ◽  
Soft Tissues ◽  
Medullary Canal ◽  
Control Group ◽  
Tibial Osteotomy ◽  
Bone Fragments ◽  
Hematoxylin And Eosin ◽  
12X18h9t Steel ◽  
Incomplete Closure ◽  
Experimental Group

Aim. To perform comparative and morphological assessment of the reparative processes in the bone and the surrounding soft tissues during intramedullary osteosynthesis using stainless steel implants and titanium nitride coated stainless steel implants.Methods. 40 rabbits who underwent open tibial osteotomy followed by retrograde insertion of the implant into the medullary canal served as an experimental model. 2 mm diameter nails of 12X18H9T steel coated with titanium and hafnium nitrides were used. In the control group, similar nails without coating were used. Histological sections of bone fragments and postoperative wounds, which were taken for morphological study on the 10th, 30th, 60th and 180th day from the beginning of the experiment, were stained with hematoxylin and eosin and with picrofuchsin by Van-Gieson method.Results.The percentage of leukocyte-necrotic masses on the 10th day was 3.5±0.2% in the experimental group and 6.0±0.4% in the comparison group (pConclusion. Osteosynthesis using nails coated with titanium and hafnium nitrides contributes to reduction of the inflammatory response in the surrounding tissues, accelerates the process of reparative regeneration, providing an earlier wound epithelialization and reducing the likelihood of complications development such as incomplete closure of bone defect.

Effect of anti-cytosolic 5′-nucleotidase 1A (NT5C1A) antibody on cultured muscle cells and muscle fibers of mice

2016 ◽  
Vol 26 ◽  
pp. S201
Author(s):  
N. Tawara ◽  
S. Yamashita ◽  
M. Korogi ◽  
X. Zhang ◽  
T. Doki ◽  
...  
Keyword(s):  
Muscle Fibers ◽  
Muscle Cells

scholarly journals THE NERVOUS ENVIRONMENT OF INDIVIDUAL SMOOTH MUSCLE CELLS OF THE GUINEA PIG VAS DEFERENS

1968 ◽  
Vol 37 (3) ◽  
pp. 794-817 ◽  
Author(s):  
Neil C. R. Merrillees
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Vas Deferens ◽  
Close Contact ◽  
Muscle Fibers ◽  
Smooth Muscles ◽  
Muscle Cells ◽  
Sensory Innervation ◽  
Close Contacts

Smooth muscle cells of the external longitudinal coat of the guinea pig vas deferens were followed for 480 µ at 4.5-µ intervals. Muscle bundles and fibers interwove, facilitating intermuscular and neuromuscular contacts. The ribbon- or rodlike muscle cells were about 450 µ long, 3,000 µ3 in volume, and 4,500 µ2 in area. The thickened nuclear zone lay anywhere along the middle one-third of the cell. Intercellular distances were 500–800 A. Intrusions were rare, and tight-junctions absent. At any level in a field of 80 muscle fibers there were 10–15 nerve bundles, each containing several varicose axons. Bundles and axons divided. Axons, en passage, were frequently within 500–1,000 A of a muscle fiber. En passage close contacts were rate. Axon terminations were bare, and bare axons invariably terminated. Bare terminations had scattered vesicle-laden varicosities and were from 10µ-60 µ in length, and all ended within 500 A of muscle fibers. Some made close contact with muscle fibers. Less than half of the muscle cells received this close contact, but some cells were approached by more than one termination. Most terminations involved more than one cell. Some cells had little or no innervation. Some groups of cells had a rich innervation. There was very little evidence of sensory innervation. These conclusions are not valid for other smooth muscles.

Human Vocalis Contains Distinct Superior and Inferior Subcompartments: Possible Candidates for the Two Masses of Vocal Fold Vibration

1998 ◽  
Vol 107 (10) ◽  
pp. 826-833 ◽  
Author(s):  
Ira Sanders ◽  
Yingshi Han ◽  
Surinder Rai ◽  
Hugh F. Biller
Keyword(s):  
Vocal Fold ◽  
Muscle Fibers ◽  
Vocal Folds ◽  
Medial Surface ◽  
Vocal Fold Vibration ◽  
Muscle Fascicle ◽  
Superior Surface ◽  
Hematoxylin And Eosin ◽  
Thyroarytenoid Muscle ◽  
Large Muscle

It is not understood how different parts of the thyroarytenoid muscle contribute to vocal fold vibration. This study investigated the medial part of the thyroarytenoid muscle, the vocalis compartment, for anatomic differences that might suggest functionally distinct areas. Twenty human vocal folds were frontally sectioned and stained with hematoxylin and eosin. A single section from the middle of each vocal fold was magnified, and the muscle fascicles of the most superficial 25% of the vocalis compartment were then examined. In all 20 specimens the vocalis compartment could be separated into 2 plainly distinct subcompartments: the inferior vocalis compartment was composed of a single large muscle fascicle that contained densely packed muscle fibers of similar size; the superior vocalis compartment was composed of multiple small fascicles in which the muscle fibers were loosely arranged and varied greatly in size. On average, the inferior vocalis subcompartment composed 60% of the medial surface of the thyroarytenoid muscle. The superior subcompartment composed the remaining 40% of the medial surface, but also continued past the vocal ligament to make up the superior surface of the thyroarytenoid muscle. It is concluded that 2 distinct entities make up the vocalis compartment of the thyroarytenoid muscle. Their anatomy is so markedly different it suggests that they may function independently. One possibility is that they reflect the 2 masses observed in the superior and inferior aspects of the vocal fold during vibration.

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