Hyperoxia effect on humoral and cell components of immunobiological resistance of healthy rats in experiment

10.12737/4112 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 1-6
Author(s):  
Мальцева ◽  
L. Maltseva
Keyword(s):  
Blood Serum ◽  
Hyperbaric Oxygen ◽  
Immune Complexes ◽  
Hyperbaric Oxygenation ◽  
Theoretical Explanation ◽  
The Body ◽  
Circulating Immune Complexes ◽  
Complement Activity ◽  
Early Stages ◽  
Cell Components

The paper contains information about hyperbaric oxygenation impact on humoral and cell components of immune-biological resistance of healthy rats. The following factors are analyzed: potency of oxygen-dependent enzyme systems (myeloperoxidase, oxidase), oxygen-independent systems (leukocyte lysozyme, cationic proteins), killing reactions of peripheral blood neutrophils, their phagocytic and oxidase reserve, complement activity and content of circulating immune complexes in the blood serum of healthy animals, which have been exposed to 60-minute sessions of hyperbaric oxygenation, over time from the 1st to the 30th day post hyperoxia period during experiment. A single session of hyperbaric oxygenation is carried out within 60 minutes (3 absolute atmospheres mode). Mechanisms of adaptation and pathological reactions formation by immune-biological surveillance system of healthy body in early and late post hyperoxia periods during experiment are considered. The paper shows that adaptive responses, which are designed to protect the body against aggressive action of oxygen under the influence of hyperbaric oxygenation, are formed in the bodies of healthy animals. The reactions of oxygen-dependent of phagocytes systems in conditions of hyperoxia manifested in the early stages of the research, persisting to the end of the observation and the reactions of oxygen independent - only at early stages. The humoral responses of healthy organism under hyperbaric oxygen are characterized by the change in complement activity and concentration of circulating immune complexes in blood serum. The received experimental data may be used as a theoretical explanation of indications for use of hyperbaric oxygen therapy and different hyperbaric oxygenation regimes.

scholarly journals Adaptation of the status of non-specific resistance of the ducks organism in stress conditions inclusion in the ration of probiotical additives

2018 ◽  
Vol 20 (87) ◽  
pp. 32-37 ◽  
Author(s):  
V.G. Stoyanovskyy ◽  
A.О. Krogh ◽  
I.A. Kolomiіets
Keyword(s):  
Body Weight ◽  
Blood Serum ◽  
Bactericidal Activity ◽  
Immune Complexes ◽  
Specific Resistance ◽  
Lysozyme Activity ◽  
The Body ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Technological Stress

The article deals with the question of adaptation of the ducks organism during industrial cultivation for action of stress. The complex of successive stage reactions, caused by the functioning of stress-implementing systems, arise in the body of the poultry in response to the influence of destabilizing factors and aimed at neutralizing these factors, preservation of homeostasis. Increasing the influence of technological stress factors leads to a decrease in the immune status of the duck organism. The study of the effectiveness of incorporating into the diet ducks harmless feed additives, which contributes to the normalization of the course of adaptive-compensatory reactions in their body during the critical periods of postnatal ontogenesis and can serve as anti-stress prevention in conditions of industrial growth of this species of poultry remains relevant. The purpose of the work is to find out the implementation of an adaptation syndrome in the system of indices of non-specific resistance of the body of ducks when it is included in the diet of the biologically active feed supplement «Primix Bionorm K» and supplements «Biovir». The experiment was conducted on clinically healthy young ducks of the Pekin breed of egg production line. In the 270-day age, all three groups of ducks were modeled experimental technological stress in the form of prolonged transport. From 240 to 270 days of age, ducks additionally fed the additive «Primix Bionorm K» and was pouring with water an additive «Biovir». The material for research was blood, which was taken until morning feeding after slaughter on 240 days of life (to stress), 270 days of life (stage of anxiety), at 273 and 285 days (stages of resistance). Adaptation of the state of non-specific resistance of the duck of the control group to the control group under conditions of stress in the anxiety stage is characterized by an increase of 17.3% (Р < 0.05) of the value of the bactericidal activity of the blood serum, a decrease of 8.5% of the value of lysozyme activity of the blood serum, by 9.3% of phagocytic activity against the background of growth on 8.7% of the number of circulating immune complexes compared to 240-day-old poultry. At the stage of resistance there is a decrease in the amount of blood serum bactericidal activity by 17.3% (Р < 0.05), an increase in the activity of lysozyme in blood serum by 8.5%, and the number of immune complexes by 20.7% (Р < 0.05), with the stabilization of the studied parameters at the later stages of the development of a stress reaction. In these periods, a decrease in the body weight of control group ducks was set at 5.7 – 6.8%. Feeding of the additive «Primix Bionorm K» during the period of the implementation of the stress syndrome has a positive effect on the indices of the cellular and humoral level of nonspecific resistance in the form of an increase in the amount of bactericidal activity of the serum on 38.2% (P < 0.05), an increase in the activity of phagocytosis by 15.1% (P < 0.05), phagocytic index – by 36.1% (P < 0.05) with a decrease in the number of circulating immune complexes by 15.5%. The presentation of the additive «Biovir» causes an increase in the level of bactericidal and lysozyme activity of the blood serum on 47.3% (P < 0.01) and 18.7% (P < 0.05). The use of additives can reduce the negative effect of transport stress in the duck body, increase nutrient uptake, as indicated by an increase in the body weight of experimental birds in the average by 7.1–10.5% (P < 0.05) during the development of the adaptive syndrome.

scholarly journals Influence of cryptosporidiosis agent on biochemical and immunological parameters of blood of calves

2018 ◽  
Vol 20 (88) ◽  
pp. 136-140 ◽  
Author(s):  
V. V. Zhurenko ◽  
O. V. Zhurenko
Keyword(s):  
Immune System ◽  
Blood Serum ◽  
Immune Complexes ◽  
Structural Changes ◽  
Specific Interaction ◽  
The Body ◽  
Circulating Immune Complexes ◽  
Total Protein Content ◽  
Immunological Parameters ◽  
Antigen Antibody

Cows, young cattle and calves of black-and-bark breeds, spontaneously affected by the causative agent Cryptosporidium parvum, were selected for the research. Blood for research was taken from calves in the morning before feeding from the jugular vein. Biochemical parameters of blood serum were determined using a biochemical analyzer of the closed type. Changes in the activity of serum enzymes in the initial stages of the disease are not specific, they indicate a compensatory reaction of liver cells and biliary tract. Effect of Cryptosporidium in calves body accompanied by severe disorders that affect the metabolism of proteins, carbohydrates and enzymes. These changes lead to structural changes in the organs at the cellular level. Serum infested animals significantly reduced total protein content of 25.5% (P < 0.001), albumin – by 14.6% (P < 0.01), the concentration of glucose in – 21.2% (P < 0.01), carotene content – 12.6% (P < 0.05), the level of calcium and phosphorus – 17.5 and 18% (P < 0.05), respectively, and increased total bilirubin content by 23% (P < 0.001), indicating significant violations of the protein synthesis and detoxification processes in the liver and the tension in the metabolism of their body. Reduced glucose concentration in blood serum is due to the fact that in the body of sick animals there have been increased costs for maintaining the energy needs of its own organism. In conducting studies for 35 days in animals in the experimental group noted an increase in the concentration of circulating immune complexes by 10.5% relative to control. Increasing the concentration of circulating immune complexes in serum of calves, patients with cryptosporidiosis, indicates the presence of specific interaction antigen-antibody and decrease the activity of the humoral link of the immune system. In the determination of immunosuppressive proteins – serum cords in serum of calves, it was found that at 7, 14 days their concentration was within the physiological limits. Increasing the concentration of circulating immune complexes in serum of calves, patients with cryptosporidiosis, indicates the presence of specific interaction antigen-antibody and decrease the activity of the humoral link of the immune system.

scholarly journals Influence of Marmix premix on the state of lipid peroxidation and indices of non-specific resistance of the organism of pregnant mares with microelementosis

2019 ◽  
Vol 10 (1) ◽  
pp. 87-91
Author(s):  
A. R. Shcherbatyy ◽  
L. G. Slivinska ◽  
B. V. Gutyj ◽  
V. L. Fedorovych ◽  
B. O. Lukashchuk
Keyword(s):  
Lipid Peroxidation ◽  
Blood Serum ◽  
Immune Complexes ◽  
Positive Influence ◽  
Lysozyme Activity ◽  
The State ◽  
Circulating Immune Complexes ◽  
Lipid Hydroperoxides ◽  
Diene Conjugates ◽  
Lipid Peroxidation Products

We observed the processes of lipid peroxidation, characterized by increased content of intermediate and its end products (diene conjugates, lipid hydroperoxides, malonic dialdehyde), in the blood of the pregnant mares. The changes of the data of indicators in the organism of the mares at the 9–11 months of pregnancy were established. The level of diene conjugates in the blood of animals was 3.6 ± 0.12 μmol/l, 33.3 and 44.0%, which was higher compared to non-pregnant mares and mares at 4th month of pregnancy. The concentration of lipid hydroperoxides in the blood of mares increased by 100% and by 42.9% in the blood of non-pregnant mares and mares at 4th month of pregnancy. The concentration of malondialdehyde in the blood of mares increased by 75.0%, 51.2% and 25.0% compared to non-pregnant mares and mares at 4th and 7th month of pregnancy, respectively. The results of our research showed that the mineral-vitamin premix Marmix had a positive influence on the state of lipid peroxidation products, contributed to their lowering in the blood of pregnant mares, which demonstrated antioxidant action and stimulated the function of the immune system. It was established that the bactericidal and lysozyme activity of the blood serum and the concentration of circulating immune complexes increases in the mares with lack of trace elements. It was shown that feeding mares during the 9–11 months of pregnancy with the mineral-vitamin premix Marmix had a positive influence on the state of humoral factors of nonspecific resistance. In particular, the concentration of bactericidal activity of serum increased by 31.0%, lysozyme activity of blood serum – 45.4% and contributed to the decrease of the concentration of circulating immune complexes in serum by 3.8 times. The use of mineral-vitamin premix Marmix during 60 days in feeding pregnant mares caused a restoration of the clinical status, a decrease in the content of lipid peroxidation products.

scholarly journals Identification of markers of opportunistic infections in frequently ill children in the North-Eastern regions of Russia

2019 ◽  
Vol 18 (4) ◽  
pp. 5-11 ◽  
Author(s):  
N. V. Karazhas ◽  
L. V. Feklisova ◽  
T. A. Semenenko ◽  
M. N. Kornienko ◽  
T. N. Rybalkina ◽  
...  
Keyword(s):  
Immune Complexes ◽  
Opportunistic Infections ◽  
Solid Phase ◽  
Acute Infection ◽  
Physiological Mechanism ◽  
The Body ◽  
Circulating Immune Complexes ◽  
The North ◽  
North Eastern ◽  
Regions Of Russia

The study is devoted to the clinical examination of frequently ill children in the North-Eastern regions of Russia and the laboratory detection of markers of herpesvirus infections, as well as pneumocystosis and the establishment of the relationship with their immune status.62 children aged 8—15 years were examined. Samples of serum and blood cells, sputum on markers of herpesvirus infections and pneumocystosis by ELISA, indirect immunofluorescence reaction and rapid culture method were studied. Assessment of humoral immunity and determination of circulating immune complexes was performed by solid-phase enzyme immunoassay and immune turbodimetric analysis.The study shows the etiological role of herpesviruses. Active infection (acute, reactivation) was caused in 9.7% of cases of HSV-1,2, 12.9% — EBV, 6.5% — CMV, 19.4% — HHV-6. This is indicated by a large number of convalescents: with EBVI — 40.3%, HSVI — 27.4%, HHVI-6 — 8.1%, CMVI — 4.8%. Also, a significant number of children were found and latent form. The formation of the circulating immune complexes (CIC) is a physiological mechanism of protection of the body, and their excessive accumulation indicates the development of inflammatory processes and autoimmune diseases. In the increase in CIC observed mainly in individuals with latent infection: HSVI — 29.4%; HHVI-6 — 20.8%; CMVI — 16.7%, EBVI — 11.9%. It is important to note that in almost half of the cases the increase in CIC level occurred simultaneously with the increase in the concentration of total IgE. A survey of children on Pneumocystis showed that among them, dominated native — 11.3%, in which the sputum was identified pnevmotsisty. It should be noted that one child was found mixed infection with reactivated CMVI. Acute infection was diagnosed only in 6.4% of the patients.

DYNAMICS OF THE LEVEL OF CIR-CULATING IMMUNE COMPLEXES IN BLOOD SERUM IN RABBITS UNDER MODEL STRESSES

2020 ◽  
Vol 3 ◽  
pp. 135-138
Author(s):  
O. V. Kryachko ◽  
◽  
L.A. Lukoyanova ◽  
Keyword(s):  
Blood Serum ◽  
Immune Complexes ◽  
Live Weight ◽  
Circulating Immune Complexes ◽  
Stress Factors ◽  
Innate Immune Responses ◽  
Stress Effects ◽  
Transport Stress ◽  
Post Stress ◽  
Dorsal Position

A small amount of circulating immune complexes is determined in a healthy body, and it naturally increases when there is an antigen in the circulation. Stressful situations can inhibit the implementation of immune protection, both adaptive and innate immune responses. The purpose of this study was to study the dynamics of the level of circulating immune complexes in blood serum after modeling single and combined stress effects in rabbits. 10 male rabbits were used for the experiment. The animals were 8 months old and had a live weight of 3.40±0.73 kg. Transport stress was caused by transporting animals in a car. Immobilization stress was modeled by fixing rabbits in a fixation de-vice for small animals by their legs in the dorsal position. In the final series of experi-ments, a combination of actions was per-formed (immobilization, then, after three days, transportation). As a result of studies it was found that the reaction of animals to stress was characterized by decreased level of the CIC, during transportation (by 1.2 times (p<0.05) and immobilization (1.6 times, p<0.05), the combination of stress factors showed a similar effect. In the post-stress period, we observed in all cases an increase in the CIC level in the blood of rab-bits, which exceeded the CIC values both at the time of the stress reaction and the level of intact animals.

scholarly journals Molecular Aggregation in Immune System Activation Studied by Dynamic Light Scattering

Biology ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 123
Author(s):  
Elena Velichko ◽  
Sergey Makarov ◽  
Elina Nepomnyashchaya ◽  
Ge Dong
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Blood Serum ◽  
Immune Complexes ◽  
Circulating Immune Complexes ◽  
Scattering Method ◽  
Dynamic Light Scattering Method ◽  
Immune System Activation ◽  
Healthy Donors ◽  
Biomedical Diagnostics

Determination of the concentration and size of the circulating immune complexes in the blood is an essential part of diagnostics of immune diseases. In this work, we suggest using the dynamic light scattering method to determine the sizes of circulating immune complexes in blood serum. By the dynamic light scattering spectrometer, we found that for healthy and sick donors, the size and concentration of circulating immune complexes differed significantly. The dynamics of formation of these complexes were also examined in this work. It was shown that the formation of immune complexes in the blood of healthy donors is faster than the same reactions in the blood serum of donors with diseases. The results can be used in the diagnostics of the immune status and detection of chronic inflammation. We can recommend the dynamic light scattering method for implementation in biomedical diagnostics.

scholarly journals Indicators of total homeostasis in cows in different periods of lactations

2021 ◽  
Vol 51 (2) ◽  
pp. 56-64
Author(s):  
E. A. Dementyeva ◽  
S. N. Mager
Keyword(s):  
Immune Complexes ◽  
Relative Number ◽  
The Body ◽  
Mononuclear Phagocytes ◽  
Circulating Immune Complexes ◽  
High Rate ◽  
Control Group ◽  
Dry Period ◽  
Birth Process ◽  
Significant Difference

The immunomorphological parameters of cattle in different periods of lactation were studied. In the first 3 months of lactation, excluding 7 days of the colostrum period, 59.3 units of circulating immune complexes were found in the serum, in the middle of lactation (4–7 months) – 94.2 (p <0.05), at the end (8–10 months) – 94.1 units (p <0.05). The significant difference between the indicator in the first 3 months and in the subsequent periods of lactation is due to the fact that at the beginning of lactation the cows were not yet pregnant. In the colostrum period, a high rate of circulating immune complexes of 116.1 units (p <0.05) was determined as a consequence of a fetal prenatal immune attack on the cow's body, when the system of mononuclear phagocytes had not yet coped with the elimination of neutralization products. During the dry period, the number of circulating immune complexes was 87.6 units (p <0.05). The decrease in the indicator occurred due to an increase in the activity of mononuclear phagocytes and the absence of lactation effect on the body. The content of segmental, functionally mature leukocytes at the beginning of lactation was 39.4%, in the middle of lactation this indicator decreased to 24.8% (p<0.05), at the end it was 26.3% (p<0.05). A significant difference was found in the relative number of segmented neutrophils in the control group and in non-lactating cows in the dry period – 29.9% (p <0.05). During the colostrum period, the level of lymphocytes in the blood of animals was 62.0% (p <0.05) and it significantly differed from the control – 43.6%. In the middle and at the end of lactation, there was also a significant difference between the indicator and the control, up to 58.9–59.4% (p<0.05). A significant difference with the group of down-calving dry cows was established – 53.9% (p <0.05). In the first 1–3 months of lactation, cows are either not yet pregnant, or a close bond (placenta) between the mother and the fetus has not yet been formed, therefore a low activity of specific immunity at this time is caused by the absence of foreign fetal antigens in the blood of cows. The findings suggest that the birth process may be initiated by the immune system.

scholarly journals Peculiarities of the formation and subsequent removal of the circulating immune complexes from the bloodstream during the process of digestion

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 618
Author(s):  
Sergej B. Landa ◽  
Pavel V. Korabliov ◽  
Elena V. Semenova ◽  
Michael V. Filatov
Keyword(s):  
Immune Complexes ◽  
Biological Process ◽  
Biological Fluids ◽  
The Body ◽  
Circulating Immune Complexes ◽  
Physical Parameters ◽  
Kinetic Characteristics ◽  
Large Protein ◽  
Subsequent Removal ◽  
Cellular Mechanisms

Background: Large protein aggregates, known as circulating immune complexes (CICs), are formed in biological fluids as a result of the development of the body's immune response to various provoking factors. The kinetic characteristics of the formation and removal of immune complexes (ICs), their physical parameters, the isotypic composition of immunoglobulins (Igs) and the antigenic component of the CICs may reflect certain aspects of certain pathological and metabolic processes taking place in humans and animals. The aim of this study is to assess the kinetic characteristics of the formation and removal of the CICs that form in blood after eating. We also analyze the changes in the isotypic composition of Igs of ICs that accompany this biological process in rodents and humans. Methods: We identified the CICs, which differed in size and class of Igs, using dynamic light scattering. To remove ICs from the plasma, we used immune-affinity sedimentation. Monoclonal antibodies for the Igs of different isotypes were added to the plasma samples to determine the isotypic composition of the ICs. Results: A large number of ICs were formed in the blood of rats and humans after eating (food CICs). In rats, food ICs are almost immediately filtered in the liver, without circulating in the bloodstream through the body. In humans, the level of food ICs in the blood increases for 3.5 h after ingestion, then within 7–8 h their gradual removal takes place. It was found that in the process of digestion in humans, the isotypic composition of Igs in the CICs changes and becomes more diverse. Conclusions: The molecular–cellular mechanisms of the formation and utilization of food CICs in humans and rodents do not match completely.

Assessment of the immunological status in patients of a surgical hospital

1993 ◽  
Vol 74 (3) ◽  
pp. 218-219
Author(s):  
I. V. Klyushkin ◽  
E. E. Krasnoshchekova ◽  
K. T. Valeeva ◽  
M. N. Nasrullaev
Keyword(s):  
Immune System ◽  
Blood Serum ◽  
Conventional Method ◽  
Bactericidal Activity ◽  
Immune Complexes ◽  
Clinical Picture ◽  
Circulating Immune Complexes ◽  
Immunological Status ◽  
Serum Immunoglobulins

In patients with a surgical profile, the humoral link of the immune system was studied for the production of 3 classes of immunoglobulins (IgA, IgM, IgG), the formation of circulating immune complexes (CIC), bactericidal activity of blood serum (BASK) in comparison with the clinical picture of the disease. Serum immunoglobulins were determined according to Mancini, CEC by the conventional method according to D. K. Novikov (1987), BASK by the method of V. I. Nikitenko and O. V. Bukharin.

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