Biochemical Analysis of Vitreous Humor in Postmortal Diagnosis of Renal Failure

10.12737/2726 ◽  
2013 ◽  
Vol 20 (4) ◽  
pp. 47-49
Author(s):  
Терёхина ◽  
N. Terekhina ◽  
Акимов ◽  
Pavel Akimov
Keyword(s):  
Diabetes Mellitus ◽  
Molecular Weight ◽  
Renal Failure ◽  
Serum Creatinine ◽  
Biochemical Analysis ◽  
Vitreous Humor ◽  
Control Group ◽  
Patients With Diabetes ◽  
Maximum Content

The content of medium molecular weight peptides and creatinine in serum and vitreous humor from 48 corpses of people suffering from diabetes mellitus during his lifetime and 27 without this disease, formed the control group, were investigated. The presence of renal failure among patients with diabetes mellitus and in the control group was, respectively, in 20 and 15 persons was revealed. The content of serum creatinine in cadaver blood without renal failure was more than 2-2,5 - folds in comparative with living people and was not more than 240 μηΊθΙ/L. The content of serum medium molecular weight peptides in control group was not more than 2,8 g/L, and in the group of diabetes mellitus without renal failure - 2,9 g/L. The obtained results are analogical with data of living people accordingly literature sources. Direct correlation between the content of creatinine, medium molecular weight peptides and vitreous humor and serum are estimated. The maximum content of creatinine and medium molecular weight peptides in vitreous humor were, respectively, 110 μΓηοΙ/L and 0,5 g/L. The values extreme graduated in presents of renal failure as in serum, as in vitreous humor. Thus, in postmortal period appropriate to determine the vitreous humor creatinine and medium molecular weight peptides content for the diagnosis of renal failure.

scholarly journals The Study of Biochemical Markers in Chronic Renal Failure Patients in Pre- and Post-Haemodialysis

2021 ◽  
Vol 53 (03) ◽  
pp. 259-264
Author(s):  
Mohammed Abed Jawad ◽  
Keyword(s):  
Diabetes Mellitus ◽  
Lipid Peroxidation ◽  
Renal Failure ◽  
Chronic Renal Failure ◽  
Group Method ◽  
Control Group ◽  
Renal Markers ◽  
Patients With Diabetes ◽  
History Of ◽  
Notable Increase

Background: The current research is designed to investigate alterations in lipid peroxidation (malondialdehyde - MDA) and renal markers (urea and creatinine) in patients of chronic renal failure (CRF) as compared to the control group. Method: The study included 55 subjects, who were separated into two groups: control group, which included 15 healthy members with no history of systematic illness; and patients group, which included 40 patients with CRF divided into four groups “Non: CRF patients without any accompanied disease, DM: CRF patients with diabetes mellitus, HT: CRF patients with hypertension, and HT + DM: CRF patients with diabetes mellitus and hypertension”. Results: The findings reveal that there is a notable increase in serum concentration of MDA, urea, and creatinine, in patients group as compared to the control group. Conclusion: In all chronic renal failure patients with or without any accompanying disease, lipid peroxidation is present in pre- and post-haemodialysis patients as well as patients with CRF have high levels of urea and creatinine compared with healthy groups.

scholarly journals The significance of resistin concentration in metabolical diseases

2010 ◽  
Vol 67 (12) ◽  
pp. 965-968
Author(s):  
Dragisa Rasic ◽  
Radojica Stolic ◽  
Aleksandar Jovanovic ◽  
Goran Trajkovic ◽  
Julijana Rasic ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Renal Failure ◽  
Waist Circumference ◽  
Renal Insufficiency ◽  
Biochemical Analysis ◽  
Metabolic Diseases ◽  
Arterial Blood ◽  
Significant Difference ◽  
Patients With Diabetes ◽  
Active Substances

Background/Aim. Adipose tissue secrets a number of hormonal active substances that play an important role in the development of various metabolic diseases. The aim of this study was to determine the resistin significance in patients with various forms of metabolic diseases. Methods. The survey was conducted in a hospital in Laplje Selo, and included 102 patients suffering from diabetes mellitus, cardiovascular disease and renal insufficiency. All patients were verified and anthropometric characteristics and the basic hematological and biochemical analysis and resistin level were controlled. Results. The average age of the patients, 69 (67.6%) men and 33 (32.4%) women, was 55 ? 9.3 years. It was about two-thirds of smokers. The values of mean arterial blood pressure suggested that our patients had expressed hypertension, body mass index (28.5 ? 3.4 kg/m2) pointed to excessive well-fed, the circumference of hips (100 ? 10.8 cm), waist circumference (110 ? 17.4 cm) and the ratio of hip/waist circumference (1.05 ? 0.14) indicated a significant intra-abdominal distribution of fat tissue. More than one third of our patients had a certain form of cardiovascular diseases, renal failure was present almost in a fifth of patients, while diabetes mellitus was verified in 44.1% of the studied population. Significantly higher values of resistin concentrations were determined in patients with renal insufficiency and diabetes mellitus. In relation to the level of resistin, according to the number of metabolic diseases per patient, a statistically significant difference was achieved (F = 11.80, DF = 4.61, p < 0.001). Spearman correlation (r = 0.66, p < 0.001) found a statistically significant correlation between the number of diseases in one subject and the levels of resistin. Conclusion. In patients with diabetes mellitus and renal failure higher concentrations of resistin were recorded.

Apolipoprotein(a) is Present in Urine and Its Excretion is Decreased in Patients with Renal Failure

1992 ◽  
Vol 38 (11) ◽  
pp. 2244-2248 ◽  
Author(s):  
K Oida ◽  
H Takai ◽  
H Maeda ◽  
S Takahashi ◽  
A Shimada ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Renal Failure ◽  
Renal Function ◽  
Serum Creatinine ◽  
Creatinine Clearance ◽  
Renal Dysfunction ◽  
Filtration Rate ◽  
Apolipoprotein A ◽  
Patients With Diabetes ◽  
Serum And Urine

Abstract To investigate the relation between renal function and concentrations of lipoprotein(a) [Lp(a)] in serum, we measured Lp(a) in samples of serum and urine from patients with diabetes mellitus and in samples sent to a laboratory center for measurements of creatinine clearance. Serum Lp(a) concentrations were significantly increased in subjects with obvious renal dysfunction (serum creatinine &gt; or = 176.8 mumol/L) compared with normal control subjects. Urinary Lp(a) excretion was decreased in subjects with obvious renal dysfunction compared with subjects without obvious renal dysfunction (serum creatinine &lt; or = 88.4 mumol/L) and was negatively and positively correlated with serum creatinine and creatinine clearance, respectively. More than 80% of urinary Lp(a) was recovered in the d &gt; 1.21 kg/L fraction. At least six bands for apolipoprotein(a) [apo(a)] fragments, which were smaller than native apo(a) in serum, were observed in urine by immunoblotting, and some of these were also detected in serum. Degraded apo(a) fragments are probably present in urine, and their excretion decreases in parallel with decreases in the glomerular filtration rate.

Soluble Fibrin Complexes and Fibrinogen Heterogeneity in Diabetes mellitus

1980 ◽  
Vol 44 (03) ◽  
pp. 130-134 ◽  
Author(s):  
E B Tsianos ◽  
N E Stathakis
Keyword(s):  
Diabetes Mellitus ◽  
Molecular Weight ◽  
Plasma Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gel Filtration ◽  
Lower Molecular Weight ◽  
Polyacrylamide Gels ◽  
Soluble Fibrin ◽  
Α2 Macroglobulin ◽  
Patients With Diabetes

SummaryThe presence of soluble fibrin complexes (SFC) measured by gel filtration of plasma on 4% agarose columns, fibrinogen heterogeneity on 3.5% SDS-polyacrylamide gels and the concentrations of several plasma proteins were evaluated in 39 patients with diabetes mellitus (DM) and 19 matched control subjects. A small but significant increase of SFC was found in DM (p<0.01). On individual basis 51.2% of the patients had increased SFC (>M + 2 SD of the controls). Polyacrylamide gel electrophoresis of the SFC showed no evidence of cross-linking or proteolysis. Plasma clots formed in the presence of EDTA and trasylol were analysed in SDS-polyacrylamide gels in a normal and two lower molecular weight fibrin bands (band I, II, III). The percentage of band I fibrinogen was in diabetics (65.3 ± 4.7%) lower than that of the controls (71.8 ± 4.5%) (p < 0.01). Fibrinogen levels, antithrombin III, α1-antitrypsin, α2-macroglobulin and plasminogen were significantly increased in DM. We suggest that in DM there is an enhancement of intravascular fibrin formation and accelerated fibrinogen degradation to lower molecular weight forms.

SUPPORTIVE EDUCATIVE SYSTEM DALAM MENINGKATKAN KEMANDIRIAN MERAWAT KAKI PADA PASIEN DIABETES MELLITUS TIPE II

2019 ◽  
Vol 9 (2) ◽  
pp. 63-72
Author(s):  
Nova Nurwinda Sari ◽  
Herlina Herlina
Keyword(s):  
Diabetes Mellitus ◽  
Health Center ◽  
Intervention Group ◽  
T Test ◽  
Control Group ◽  
P Value ◽  
Type Ii ◽  
Foot Care ◽  
Control Group Design ◽  
Patients With Diabetes

Diabetes mellitus dapat menyebabkan cukup banyak komplikasi seperti kelainan mata, kelainan ginjal, kelainan pembuluh darah dan kelainan pada kaki. Penderita diabetes mellitus yang mengalami komplikasi kronis perlu diberikan upaya preventif untuk mencegah komplikasi, salah satunya adalah kemampuan perawatan kaki. Penelitian ini dilakukan untuk menguji efektivitas supportive educative system dalam meningkatkan kemandirian perawatan kaki pada pasien dengan diabetes mellitus Tipe II di Puskesmas Permata Sukarame, Bandar Lampung. Penelitian ini menggunakan metode quasy eksperimen dengan desain pretest-posttest with control group dengan total masing-masing kelompok sebanyak 18 responden. Pengumpulan data dilakukan dengan menyebarkan kuesioner kepada responden yang memenuhi kriteria inklusi penelitian. Penelitian ini diuji menggunakan analisis univariat, bivariat dan uji T-test. Hasil penelitian menunjukkan bahwa terdapat perbedaan rata-rata dalam kemandirian perawatan kaki pada kelompok intervensi dan kelompok kontrol dengan p-value 0,000. Pendidikan dan praktik perawatan kaki harus diberikan sejak dini sebagai upaya pencegahan untuk komplikasi.   Kata kunci : Supportive educative system, kemandirian perawatan kaki   SUPPORTIVE EDUCATIVE SYSTEM IN IMPROVING INDEPENDENCE OF FOOT CARE IN PATIENTS WITH DIABETES MELLITUS TYPE II   ABSTRACT Diabetes mellitus can cause quite a lot of complications such as eye disorders, kidney disorders, vascular disorders and abnormalities in the legs. Patients with diabetes mellitus who have chronic complications need to be given a preventive effort to prevent complications, one of which is foot care ability. This research was conducted to examine the effectiveness of supportive educative systems in increasing the independence of foot care in patients with Type II diabetes mellitus in the Permata Sukarame Health Center Bandar Lampung Working Area. This study used a quasi-experimental method with pretest-posttest with control group design with a total of 18 respondents each. Data collection is done by distributing questionnaires to respondents who meet the research inclusion criteria. This study was tested using univariate, bivariate, T-Test analysis. The results showed that the mean differences in the independence of foot care in the intervention group and the control group in the Permata Sukarame Community Health Center work area with a p-value of 0,000. Education and practice of foot care should be given early as a preventative effort for complications.   Keywords: Supportive educative system, independence of foot care

Diabetes-induced Alterations in HDL Subfractions Distribution

2020 ◽  
Vol 26 (27) ◽  
pp. 3341-3348 ◽  
Author(s):  
Marek Femlak ◽  
Anna Gluba-Brzozka ◽  
Beata Franczyk ◽  
Jacek Rysz
Keyword(s):  
Diabetes Mellitus ◽  
Cardiovascular Risk ◽  
Public Health Problem ◽  
Statin Treatment ◽  
Oxidative Modification ◽  
Control Group ◽  
Newly Diagnosed ◽  
Hdl Subfractions ◽  
Patients With Diabetes ◽  
Healthy Control

Introduction: Diabetes mellitus (DM) due to its increasing prevalence and associated morbidity and mortality has become a serious public health problem. In DM, HDL may lose its beneficial features and become proatherogenic due to its altered biological activity thus increasing cardiovascular risk. The aim of this study was to assess the influence of the presence of diabetes mellitus type 2 and its duration on the distribution of HDL subfractions. Moreover, the effect of statin treatment on HDL subfraction share was analysed in this study. Methods: The study group consisted of 50 patients with newly diagnosed DM and 50 persons with DM for longer than 10 years while the control group consisted of 50 healthy volunteers. HDL subfractions were analysed with the use of Lipoprint. Results: We demonstrated progressive worsening of heart functioning and impairment of its structure in the course of diabetes mellitus. Moreover, we observed that HDL-6 subfraction and intermediate HDL fraction are lowest in the group with advanced DMt2 compared to the group with newly diagnosed DM and a healthy control group. Finally, the results of our study indicated the effect of statin treatment on HDL subfractions that seems not to be advantageous. Conclusion: It seems that in patients with diabetes mellitus compromised antiatherogenic properties of HDL, as a result of oxidative modification and glycation of the HDL protein as well as the transformation of the HDL proteome into a proinflammatory protein, increase cardiovascular risk.

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Type 2 Diabetes Mellitus: A Retrospective Cohort Study

2020 ◽  
Vol 20 (4) ◽  
pp. 584-590 ◽  
Author(s):  
Shima Fathi ◽  
Shiva Borzouei ◽  
Mohammad Taghi Goodarzi ◽  
Jalal Poorolajal ◽  
Fatemeh Ahmadi-Motamayel
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Type 2 Dm ◽  
Patients With Diabetes ◽  
The Difference ◽  
And Oxidative Stress

Background: Diabetes Mellitus (DM) is a progressive metabolic disorder. Objective: The aim of this study was to investigate the relationship between antioxidant and oxidative stress markers in the saliva of patients with type 2 DM and a healthy control group. Methods: In this study, 20 patients with diabetes and 20 healthy individuals were evaluated. Salivary antioxidants markers consisted of total antioxidant capacity (TAC), uric acid (UA), peroxidase and catalase. Oxidative stress markers included total oxidant status (TOS), malondealdehyde (MDA) and total thiol (SH). Sialochemical analysis was performed with spectrophotometric assay. All the statistical analyses were conducted using STATA software. Results: TAC decreased significantly in patients with diabetes. Although salivary UA and peroxidase were lower in patients with diabetes compared to the control group, the difference was not significant. Salivary catalase in patients with diabetes was significantly lower than that in the control group. MDA and TOS exhibited significantly higher levels in type 2 DM. SH levels were slightly higher in DM. Conclusions: According to the results of the present study, there were some changes in the salivary levels of some antioxidants and oxidative stress markers in patients with type 2 DM and could be measured as an indicator of serum changes..

scholarly journals Hand osteomyelitis in arterial calcification, diabetes mellitus and end-stage renal failure: a comparison of 210 cases over 12 years

2021 ◽  
pp. 175319342098187
Author(s):  
Matthew Wyman ◽  
Dallan Dargan ◽  
Jennifer Caddick ◽  
Victoria Giblin
Keyword(s):  
Diabetes Mellitus ◽  
Renal Failure ◽  
Disease Free Survival ◽  
Arterial Calcification ◽  
Level Of Evidence ◽  
Free Survival ◽  
End Stage Renal Failure ◽  
Patients With Diabetes ◽  
End Stage ◽  
Steal Syndrome

We present 210 patients with hand osteomyelitis in 246 rays over 12 years, including detailed analysis of 29 patients in this cohort with digital artery calcification evident on plain X-ray. Overall 71 patients had diabetes mellitus and/or end-stage renal failure, including 28 of 29 patients with calcification. In the calcification group, 17 patients had ipsilateral arteriovenous fistulae, five had steal syndrome and 15 had digital ulceration or skin necrosis. Compared with 181 controls, patients with calcification had more affected bones, polymicrobial infections, surgical procedures, phalanges and digits amputated and had higher mortality at 1 year (12 of 29) and 5 years (20 of 29), as a result of comorbidities. Absence of calcification in 43 patients with diabetes and/or end-stage renal failure was associated with better outcomes on all the above parameters. Early amputation to maximize disease-free survival may be appropriate for patients with hand osteomyelitis and arterial calcification. Level of evidence: IV

scholarly journals Depression among patients with diabetes mellitus in a Nigerian teaching hospital

2010 ◽  
Vol 16 (2) ◽  
pp. 4 ◽  
Author(s):  
Bawo Onesirosan James ◽  
Joyce Ohiole Omoaregba ◽  
George Eze ◽  
Olufemi Morakinyo
Keyword(s):  
Diabetes Mellitus ◽  
Control Group ◽  
Depression Symptoms ◽  
Depression Symptom ◽  
Treatment Goals ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Prevalence Of Depression ◽  
Patients With Diabetes ◽  
Diabetes Patients

<p><strong>Objectives.</strong> Depression is associated with diabetes mellitus and affects treatment goals negatively. We aimed to determine the prevalence of depression and identify its socio-demographic or clinical correlates among patients with diabetes mellitus attending an out-patient clinic in Nigeria.</p><p><strong>Methods.</strong> Two hundred consecutively recruited diabetes patients (index group) were compared with a similar number of apparently healthy controls in a cross-sectional survey. In both groups, in addition to obtaining socio-demographic details, depression was diagnosed using the Schedule for the Clinical Assessment in Neuropsychiatry (SCAN), while the Beck Depression Inventory (BDI) was used to assess depression symptom severity. <strong></strong></p><p><strong>Results.</strong> Sixty (30%) diabetes patients met a SCAN diagnosis for clinical depression, compared with 19 (9.5%) in the control group. Having a smaller income and more children were significantly correlated with higher depression symptoms on the BDI.</p><p><strong>Conclusion.</strong> Depression is highly co-morbid with diabetes mellitus. The care of individuals with diabetes mellitus should include the screening and possible treatment for depression in order to achieve and sustain treatment goals.</p>

scholarly journals E-healthcare for diabetes mellitus type 2 patients – a randomised controlled trial in Slovenia

2017 ◽  
Vol 56 (3) ◽  
pp. 150-157 ◽  
Author(s):  
Rade Iljaž ◽  
Andrej Brodnik ◽  
Tatjana Zrimec ◽  
Iztok Cukjati
Keyword(s):  
Diabetes Mellitus ◽  
Blood Pressure ◽  
Nurse Practitioners ◽  
Controlled Trial ◽  
Control Group ◽  
Web Based ◽  
Patients With Diabetes ◽  
Randomised Controlled ◽  
Interventional Group

Abstract Background Telemonitoring and web-based interventions are increasingly used in primary-care practices in many countries for more effective management of patients with diabetes mellitus (DM). A new approach in treating patients with diabetes mellitus in family practices, based on ICT use and nurse practitioners, has been introduced and evaluated in this study. Method Fifteen Slovene family practices enrolled 120 DM patients treated only with a diet regime and/or tablets into the study. 58 of them were included into the interventional group, and the other 62 DM patients into the control group, within one-year-long interventional, randomised controlled trial. Patients in the control group had conventional care for DM according to Slovenian professional guidelines, while the patients in the interventional group were using also the eDiabetes application. Patients were randomised through a balanced randomisation process. Results Significant reductions of glycated haemoglobin (HbA1c) values were found after 6 and 12 months among patients using this eDiabetes application (p<0.05). Among these patients, a significant correlation was also found between self-monitored blood pressure and the final HbA1c values. Diabetic patients’ involvement in web-based intervention had only transient impact on their functional health status. Conclusion This eDiabetes application was confirmed to be an innovative approach for better self-management of DM type 2 patients not using insulin. Both a significant reduction of HbA1c values and a significant correlation between the average self-measured blood pressure and the final HbA1c values in the interventional group were found. Nurse practitioners – as diabetes care coordinators – could contribute to better adherence in diabetes e-care.

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