scholarly journals characterIstIcs of an antIoxIdant actIvIty of YERSINIA PESTIS wIth dIfferent plasmId spectrum

2016 ◽  
Vol 1 (5) ◽  
pp. 133-137
Author(s):  
Токмакова ◽  
Elena Tokmakova ◽  
Витязева ◽  
Svetlana Vityazeva ◽  
Иванова ◽  
...  
Keyword(s):  
Experimental Data ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Yersinia Pestis ◽  
Superoxide Dismutase Activity ◽  
Wild Type ◽  
Complex Study ◽  
Pathogenic Activity ◽  
The Common ◽  
High Peroxide

Experimental data concerning complex study of antioxidant activity of Y. pestis with different plasmid spectrum (wild-type Yersinia pestis subsp. pestis, Yersinia pestis subsp. altaica and their isogenic variants) are represented in the article. Superoxide dismutase activity of the tested Y.pestis strains was from 6.0 to 9.0×109 microbe cells. Signifi-cant differences between the Y.pestis strains with different plasmid composition were not detected by this parameter. Our results, consistent with the data of other authors, tell that superoxide dismutase activity is a thermo-inducible feature and does nоt depend on a plasmid spectrum. High peroxide destroying activity was also detected in all tested Y.pestis strains. The differences between the strains regarding their common peroxide destroying activity were found. This parameter of the plague microbe strains lacking pYP plasmid was at least 3times lower than common peroxide destroying activity in strains with this plasmid in the genome. In our opinion these revealed differences were caused by characteristics of plasmid spectrum. The common peroxide destroying activity’s degree of Y.pestis strains can be associated with the presence of pYP plasmid in the genome. The isogenic variants of Y.pestis strains lacking one of the plasmids had smaller pathogenic activity. This fact points to the need for further study of these strains.

scholarly journals In VivoAntioxidant Activity of Deacetylasperulosidic Acid in Noni

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
De-Lu Ma ◽  
Mai Chen ◽  
Chen X. Su ◽  
Brett J. West
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Catalase Activity ◽  
Antioxidant Properties ◽  
Superoxide Dismutase Activity ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Dose Dependent

Deacetylasperulosidic acid (DAA) is a major phytochemical constituent ofMorinda citrifolia(noni) fruit. Noni juice has demonstrated antioxidant activityin vivoand in human trials. To evaluate the role of DAA in this antioxidant activity, Wistar rats were fed 0 (control group), 15, 30, or 60 mg/kg body weight per day for 7 days. Afterwards, serum malondialdehyde concentration and superoxide dismutase and glutathione peroxidase activities were measured and compared among groups. A dose-dependent reduction in malondialdehyde was evident as well as a dose-dependent increase in superoxide dismutase activity. DAA ingestion did not influence serum glutathione peroxidase activity. These results suggest that DAA contributes to the antioxidant activity of noni juice by increasing superoxide dismutase activity. The fact that malondialdehyde concentrations declined with increased DAA dose, despite the lack of glutathione peroxidase-inducing activity, suggests that DAA may also increase catalase activity. It has been previously reported that noni juice increases catalase activityin vivobut additional research is required to confirm the effect of DAA on catalase. Even so, the current findings do explain a possible mechanism of action for the antioxidant properties of noni juice that have been observed in human clinical trials.

scholarly journals Prooxidant-Antioxidant Balance in the Heart of Rats with Different Behavioral Activities under the Conditions of Light Exposition

2021 ◽  
Vol 6 (3) ◽  
pp. 360-365
Author(s):  
L. D. Chebotar ◽  
◽  
E. N. Laricheva ◽  
M. Sh. Gilmutdinova
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Open Field ◽  
Emotional Stress ◽  
Light Exposure ◽  
Superoxide Dismutase Activity ◽  
Main Role ◽  
Experimental Animals ◽  
Behavioural Activity

The purpose of the article. The article shows that the effect of round-the-clock lighting causes changes in the processes of lipid peroxidation and antioxidant activity in rats, and depends on their behavioural activity. The effects of lighting on the processes of oxidative metabolism of varying degrees in the heart of resistant and unstable to emotional stress rats. Materials and methods. Investigations were carried out on 32 mature Wistar rats, divided into four groups: group 1 – animals resistant to emotional stress (intact); group 2 – intact animals unstable to emotional stress; group 3 – experimental animals resistant to emotional stress (30-day light exposure (1000 lux)); group 4 – experimental animals, unstable to emotional stress (30-day light exposure (1000 lux)). When assessing the effect of light on the state of the organism, the most important integral indicator is the behavior of animals. Therefore, during the experiment we used the observation of behavioral reactions in the test “open field”. Based on the characteristics of the behavior of animals in the “open field” rats were divided into groups resistant and unstable to emotional stress. To assess lipid peroxidation in the heart homogenate, the concentration of TBA-active products, the concentration of diene, oxidiene and triene conjugates were determined. Antioxidant processes were assessed by the increase in the concentration of TBA- active products during 1.5-hour incubation in an iron-ascorbate buffer solution, as well as by the activity of superoxide dismutase and catalase. Results and discussion. Prooxidant activity was characterized by an increase in the concentration of TBA-reactive substances in animals resistant to emotional stress. The concentration of TBA-reactive substances after 1.5-hour incubation increased in both experimental groups. Changes in the antioxidant status were illustrated by an increase in superoxide dismutase activity in the group of stress-unstable rats, whereas catalase activity increased in both experimental groups. In addition, in the group of animals resistant to emotional stress, a significant decrease in the resources of α-tocopherol and β-carotene was revealed. Conclusion. The long-term light exposure promotes the formation of end products of peroxidation in the heart of rats resistant to emotional stress and causes a decrease in antioxidant potential, regardless of behavioural activity. Antioxidant activity in the heart of emotionally stress-resistant rats is realized through both the enzyme and non-enzyme links of the antioxidant defence, while the main role in the heart of emotionally stress-resistant rats is played by superoxide dismutase activity

scholarly journals Comparative Evaluation of Proteolytic and Superoxide Dismutase Activities of Yersinia pestis Strains with Different Plasmid imposition

2015 ◽  
Vol 14 (1) ◽  
pp. 83-87
Author(s):  
O. V. Yur’Eva ◽  
V. I. Dubrovina ◽  
G. B. Mukhturgin ◽  
T. A. Ivanova ◽  
K. M. Korytov ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Yersinia Pestis ◽  
Proteolytic Activity ◽  
Comparative Evaluation ◽  
Activity Level ◽  
Superoxide Dismutase Activity ◽  
Direct Dependence

Data of the proteolytic and superoxide dismutase activities of Yersinia pestis strains with different plasmid spectrum are presented. It was established that superoxide dismutase activity of the tested Y. pestis strains was not associated with its plasmid composition. Some differences were revealed concerning the proteolytic activity. Direct dependence of proteolytic activity level of the studied Y. pestis strains on pYV plasmid presence in its genome was found.

scholarly journals Antioxidant activity of the probiotic consortium in vitro

2014 ◽  
Vol 2 ◽  
Author(s):  
Saule Saduakhasova ◽  
Almagul Kushugulova ◽  
Samat Kozhakhmetov ◽  
Gulnara Shakhabayeva ◽  
Indira Tynybayeva ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Glutathione Reductase ◽  
Reductase Activity ◽  
Superoxide Dismutase Activity ◽  
Total Antioxidant Activity ◽  
Bacterial Cultures ◽  
Total Antioxidant ◽  
Glutathione Reductase Activity

Introduction: Available evidence suggests that probiotics have different biological functions that depend on several mechanisms, such as antioxidant and DNA-protective activities. The probiotic consortium includes bacterial cultures such as Streptococcus thermophilus, Lactococcus lactis, Lactobacillus plantarum, and other bacterial cultures isolated from traditional Kazakh dairy products (ayran, kumys, shubat, and healthy clinical material). The aim of this study was to investigate the total antioxidant activity of the consortium of probiotic bacteria and to determine the activity of superoxide dismutase, glutathione reductase, and DNA-protective action.Material and methods: In vitro comet assay was used to determine the antigenotoxicity of the probiotic consortium. Total antioxidant activity was determined using a method of analysis with Trolox as the equivalent. The analysis method of superoxide dismutase activity assesses the inhibition rate of the nitroblue tetrazolium reduction to formazan by superoxide dismutase. Determination of glutathione reductase activity is based on the measurement of the NADPH oxidation speed.Results: A significantly high level of the total antioxidant activity of the probiotic consortium intact cells (15.3 mM/ml) was observed whereas the activity index of  lysate  was 11.1 mM/ml.The superoxide dismutase activity of probiotic consortium lysate was evaluated, with values that peaked at 0.24 U/mg protein. The superoxide dismutase activity of the consortium was lower in comparison to L.fernentum E-3 and L.fernentum E-18 cultures with values of 0.85 U/mg and 0.76 U/mg protein, respectively. SOD activity of probiotic consortium whole cells was not observed, which is typical for lactic acid bacteria.Glutathione reductase plays an important role in the optimal protection from oxidative stress. Glutathione reductase activity of the studied probiotic consortium was low; moreover, the activity of the lysate was two times higher than the activity of the cells reaching 0.01 units/ml. Investigations by Dr. Li have shown that the intracellular glutathione may give a significant protection of Lactococcus from the damaging action of H2O2, even at very low concentrations.The data from our study suggests that the co-incubation of the epithelial cells with probiotic bacteria reduces the percentage of damaged cells (damage index–0.60).Conclusion: The studied probiotic consortium has antigenotoxic and antioxidant activities. Preparations and products of this probiotic consortium may serve as a protective component in the intestinal microbial ecosystem. 

Superoxide Dismutase Activity in Two Different Wild-Type Strains of Drosophila melanogaster

Gerontology ◽  
1981 ◽  
Vol 27 (4) ◽  
pp. 205-208 ◽  
Author(s):  
Harold R. Massie ◽  
Trevor R. Williams ◽  
Valerie R. Aiello
Keyword(s):  
Superoxide Dismutase ◽  
Superoxide Dismutase Activity ◽  
Wild Type ◽  
Type Strains ◽  
Drosophila Melanogaste

scholarly journals Antioxidant activity of L-ascorbic acid in wild-type and superoxide dismutase deficient strains ofSaccharomyces cerevisiae

Redox Report ◽  
2006 ◽  
Vol 11 (4) ◽  
pp. 179-184 ◽  
Author(s):  
Jenifer Saffi ◽  
Luciano Sonego ◽  
Queli Defaveri Varela ◽  
Mirian Salvador
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Wild Type

scholarly journals Optimization of Aging Time for Improved Antioxidant Activity and Bacteriostatic Capacity of Fresh and Black Garlic

2021 ◽  
Vol 11 (5) ◽  
pp. 2377
Author(s):  
Tsan-Chang Chang ◽  
Hung-Der Jang
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Aging Time ◽  
Superoxide Dismutase Activity ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Scavenging Capacity ◽  
Trolox Equivalent ◽  
Dpph Scavenging ◽  
Aged Garlic

To determine the optimization of aging time for improved antioxidant activity and bacteriostatic capacity of garlic during its aging, garlic produced in Yunlin region, Taiwan, was employed as the test material in an analysis of the allicin content, total phenol content, antioxidant activity, and bacteriostatic capacity of fresh and aged garlic extracts. Allicin content of the aging garlic decreased to a minor level, whereas total phenol content increased to 16.96 mg gallic acid equivalent (GAE)/mL after 35 days of the aging process. The results of antioxidant testing demonstrated favorable positive correlations among IC50 of DPPH scavenging capacity, Trolox equivalent antioxidant activity, and superoxide dismutase activity for both the fresh and aged garlic extracts. The analytical results showed that aging of garlic at 70 °C and 85% relative humidity for 40 days substantially increased the quantity of phenolics, DPPH scavenging capacity, Trolox equivalent antioxidant activity, and superoxide dismutase activity and enhanced the antioxidant activity. The extracts exhibited higher bacteriostatic capacity against Gram-positive bacteria such as Bacillus subtilis and Staphylococcus aureus than against Gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa. In conclusion, black garlic aged under the optimum conditions exhibited favorable antioxidant activity and bacteriostatic ability.

scholarly journals The Single Substitution I259T, Conserved in the Plasminogen Activator Pla of Pandemic Yersinia pestis Branches, Enhances Fibrinolytic Activity

2009 ◽  
Vol 191 (15) ◽  
pp. 4758-4766 ◽  
Author(s):  
Johanna Haiko ◽  
Maini Kukkonen ◽  
Janne J. Ravantti ◽  
Benita Westerlund-Wikström ◽  
Timo K. Korhonen
Keyword(s):  
Yersinia Pestis ◽  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Amino Acid Change ◽  
Single Amino Acid ◽  
Plasminogen Activation ◽  
Wild Type ◽  
Surface Loop ◽  
Single Amino Acid Change ◽  
The Common

ABSTRACT The outer membrane plasminogen activator Pla of Yersinia pestis is a central virulence factor in plague. The primary structure of the Pla β-barrel is conserved in Y. pestis biovars Antiqua, Medievalis, and Orientalis, which are associated with pandemics of plague. The Pla molecule of the ancestral Y. pestis lineages Microtus and Angola carries the single amino acid change T259I located in surface loop 5 of the β-barrel. Recombinant Y. pestis KIM D34 or Escherichia coli XL1 expressing Pla T259I was impaired in fibrinolysis and in plasminogen activation. Lack of detectable generation of the catalytic light chain of plasmin and inactivation of plasmin enzymatic activity by the Pla T259I construct indicated that Microtus Pla cleaved the plasminogen molecule more unspecifically than did common Pla. The isoform pattern of the Pla T259I molecule was different from that of the common Pla molecule. Microtus Pla was more efficient than wild-type Pla in α2-antiplasmin inactivation. Pla of Y. pestis and PgtE of Salmonella enterica have evolved from the same omptin ancestor, and their comparison showed that PgtE was poor in plasminogen activation but exhibited efficient antiprotease inactivation. The substitution 259IIDKT/TIDKN in PgtE, constructed to mimic the L5 region in Pla, altered proteolysis in favor of plasmin formation, whereas the reverse substitution 259TIDKN/IIDKT in Pla altered proteolysis in favor of α2-antiplasmin inactivation. The results suggest that Microtus Pla represents an ancestral form of Pla that has evolved into a more efficient plasminogen activator in the pandemic Y. pestis lineages.

Factors relating to the aerotolerance of Spirillum volutans

1986 ◽  
Vol 32 (7) ◽  
pp. 548-552 ◽  
Author(s):  
Penelope J. Padgett ◽  
Noel R. Krieg
Keyword(s):  
Superoxide Dismutase ◽  
Peroxidase Activity ◽  
Catalase Activity ◽  
Casein Hydrolysate ◽  
Superoxide Dismutase Activity ◽  
Wild Type ◽  
Aerobic Growth ◽  
Air Atmosphere ◽  
Sequential Selection ◽  
One Step

A variant of the microaerophile Spirillum volutans capable of growth under an air atmosphere (21% oxygen) was obtained by sequential selection and stabilization of colonies able to grow on a casein hydrolysate – succinate – potassium metabisulfite medium under an atmosphere of 14, 16, 18, 20, and finally 21% O2. A small amount of bisulfite (0.0025%) was still required, however, for aerobic growth. Both the variant and the wild type lacked catalase activity, and they had the same levels of superoxide dismutase activity. However, the mutant possessed 13 times more donor: hydrogen-peroxide oxidoreductase (peroxidase) activity (0.008 IU) than the wild type (0.0006 IU). An aerotolerant mutant was also isolated in one step by treatment with the mutagenic agent ethyl methane sulfonate, followed by incubation under 21% O2; it was found to possess approximately three times the peroxidase activity of the wild type. This mutant lacked catalase activity and the level of superoxide dismutase activity it contained was comparable with the wild type.

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