Factors relating to the aerotolerance of Spirillum volutans

1986 ◽  
Vol 32 (7) ◽  
pp. 548-552 ◽  
Author(s):  
Penelope J. Padgett ◽  
Noel R. Krieg
Keyword(s):  
Superoxide Dismutase ◽  
Peroxidase Activity ◽  
Catalase Activity ◽  
Casein Hydrolysate ◽  
Superoxide Dismutase Activity ◽  
Wild Type ◽  
Aerobic Growth ◽  
Air Atmosphere ◽  
Sequential Selection ◽  
One Step

A variant of the microaerophile Spirillum volutans capable of growth under an air atmosphere (21% oxygen) was obtained by sequential selection and stabilization of colonies able to grow on a casein hydrolysate – succinate – potassium metabisulfite medium under an atmosphere of 14, 16, 18, 20, and finally 21% O2. A small amount of bisulfite (0.0025%) was still required, however, for aerobic growth. Both the variant and the wild type lacked catalase activity, and they had the same levels of superoxide dismutase activity. However, the mutant possessed 13 times more donor: hydrogen-peroxide oxidoreductase (peroxidase) activity (0.008 IU) than the wild type (0.0006 IU). An aerotolerant mutant was also isolated in one step by treatment with the mutagenic agent ethyl methane sulfonate, followed by incubation under 21% O2; it was found to possess approximately three times the peroxidase activity of the wild type. This mutant lacked catalase activity and the level of superoxide dismutase activity it contained was comparable with the wild type.

scholarly journals 902 PB 517 CHANGES IN THE ACTIVITIES OF CATALASE, PEROXIDASE, AND SUPEROXIDE DISMUTASE DURING CHILLING STRESS IN ZUCCHINI SQUASH

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 563c-563
Author(s):  
Chien Yi Wang
Keyword(s):  
Superoxide Dismutase ◽  
Cold Storage ◽  
Peroxidase Activity ◽  
Catalase Activity ◽  
Cucurbita Pepo ◽  
Chilling Stress ◽  
Superoxide Dismutase Activity ◽  
Chilling Temperature ◽  
Catalase Peroxidase

The activities of catalase and superoxide dismutase decreased while peroxidase activity increased in zucchini squash (Cucurbita pepo L., cv. `Elite') during storage at 5°C. Preconditioning of squash at 15°C for 2 days prior to the cold storage reduced the decline of catalase activity and suppressed the increase in peroxidase activity. The superoxide dismutase activity remained higher in temperature conditioned squash than in untreated squash. These results indicate that acclimation to chilling temperature in squash may also involve modifications in the activities of catalase, peroxidase, and superoxide dismutase.

scholarly journals Effect of catalase inactivation on levels of inorganic peroxides, superoxide dismutase, glutathione, oxygen consumption and life span in adult houseflies (Musca domestica)

1983 ◽  
Vol 216 (2) ◽  
pp. 503-506 ◽  
Author(s):  
R G Allen ◽  
K J Farmer ◽  
R S Sohal
Keyword(s):  
Superoxide Dismutase ◽  
Oxygen Consumption ◽  
Life Span ◽  
Musca Domestica ◽  
Catalase Activity ◽  
Reduced Glutathione ◽  
Superoxide Dismutase Activity ◽  
Adaptive Responses ◽  
Total Inhibition ◽  
Rate Of Oxygen Consumption

The effects of total inhibition of catalase, induced by 3-amino-1,2,4-triazole, on the adult housefly (Musca domestica) were examined. The lack of catalase activity had no effect on the longevity of the houseflies. Inorganic-peroxide concentration was elevated at younger ages, but declined in older flies. The rate of oxygen consumption by the flies was greatly decreased and the levels of oxidized as well as reduced glutathione were augmented. Superoxide dismutase activity showed a slight increase. This study suggests that loss of catalase activity does not affect survival of houseflies due to adaptive responses.

scholarly journals In VivoAntioxidant Activity of Deacetylasperulosidic Acid in Noni

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
De-Lu Ma ◽  
Mai Chen ◽  
Chen X. Su ◽  
Brett J. West
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Catalase Activity ◽  
Antioxidant Properties ◽  
Superoxide Dismutase Activity ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Dose Dependent

Deacetylasperulosidic acid (DAA) is a major phytochemical constituent ofMorinda citrifolia(noni) fruit. Noni juice has demonstrated antioxidant activityin vivoand in human trials. To evaluate the role of DAA in this antioxidant activity, Wistar rats were fed 0 (control group), 15, 30, or 60 mg/kg body weight per day for 7 days. Afterwards, serum malondialdehyde concentration and superoxide dismutase and glutathione peroxidase activities were measured and compared among groups. A dose-dependent reduction in malondialdehyde was evident as well as a dose-dependent increase in superoxide dismutase activity. DAA ingestion did not influence serum glutathione peroxidase activity. These results suggest that DAA contributes to the antioxidant activity of noni juice by increasing superoxide dismutase activity. The fact that malondialdehyde concentrations declined with increased DAA dose, despite the lack of glutathione peroxidase-inducing activity, suggests that DAA may also increase catalase activity. It has been previously reported that noni juice increases catalase activityin vivobut additional research is required to confirm the effect of DAA on catalase. Even so, the current findings do explain a possible mechanism of action for the antioxidant properties of noni juice that have been observed in human clinical trials.

Activity of erythrocyte antioxidant enzymes in healthy women depends on age, BMI, physical activity and diet

2020 ◽  
Author(s):  
Elżbieta Cecerska-Heryć ◽  
Klaudia Krauze ◽  
Angelika Szczęśniak ◽  
Aleksandra Goryniak Mikołajczyk ◽  
Natalia Serwin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Physical Activity ◽  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Catalase Activity ◽  
Smoking Status ◽  
Superoxide Dismutase Activity ◽  
Antioxidant Systems ◽  
Healthy Women

Abstract Introduction Antioxidant enzymes protect the human body against the harmful effects of oxidative stress. The activity of antioxidant enzymes changes with age, and depends on dietary nutrients such as fats and vitamins, which can have a significant impact on minimizing or exacerbating oxidative stress. Aim Examine the effect of age, BMI, diet, physical activity and smoking status on the activity of erythrocyte antioxidant enzymes catalase, glutathione reductase, glutathione peroxidase glutathione S-transferase, superoxide dismutase and glutathione concentrations in healthy women. Material and methods This study included 98 healthy women aged between 20 and 65 years. All women underwent anthropometric tests: body weight, height, hip and waist circumference. Antioxidant activity in erythrocytes was measured by spectrophotometric methods. Results Catalase activity increased significantly with age (p<0.001), while superoxide dismutase activities and glutathione decreased with age (p =0.008, p =0.023, respectively). Women with a lower BMI (emaciation) had higher superoxide dismutase activity than those in the first degree of obesity (p = 0.009 Conclusions 1. Increased catalase activity with age may be a sign of a large amount of hydrogen peroxide, resulting from poorly functioning antioxidant systems in older age. 2. Decreased superoxide dismutase activity with age may indicate inactivation of this enzyme by excessive hydrogen peroxide, as well as glycation of superoxide dismutase molecules or reactions with lipid peroxidation products, the intensity of which increases with age. 3. The negative correlation between superoxide dismutase activity and BMI index indicates reduced enzymatic activity in obese subjects, despite increased ROS production by adipose tissue.

scholarly journals Relationship among intracellular superoxide dismutase activity, glutathione peroxidase activity, motility and morphology in human semen

2019 ◽  
Vol 112 (3) ◽  
pp. e263-e264
Author(s):  
Luchezar Vasilev Jelezarski ◽  
Dimitar Parvanov ◽  
Vilyana Georgieva ◽  
Rumiana Ganeva ◽  
Georgi Stamenov Stamenov
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Superoxide Dismutase Activity ◽  
Human Semen ◽  
Glutathione Peroxidase Activity

Superoxide Dismutase Activity in Two Different Wild-Type Strains of Drosophila melanogaster

Gerontology ◽  
1981 ◽  
Vol 27 (4) ◽  
pp. 205-208 ◽  
Author(s):  
Harold R. Massie ◽  
Trevor R. Williams ◽  
Valerie R. Aiello
Keyword(s):  
Superoxide Dismutase ◽  
Superoxide Dismutase Activity ◽  
Wild Type ◽  
Type Strains ◽  
Drosophila Melanogaste

scholarly journals characterIstIcs of an antIoxIdant actIvIty of YERSINIA PESTIS wIth dIfferent plasmId spectrum

2016 ◽  
Vol 1 (5) ◽  
pp. 133-137
Author(s):  
Токмакова ◽  
Elena Tokmakova ◽  
Витязева ◽  
Svetlana Vityazeva ◽  
Иванова ◽  
...  
Keyword(s):  
Experimental Data ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Yersinia Pestis ◽  
Superoxide Dismutase Activity ◽  
Wild Type ◽  
Complex Study ◽  
Pathogenic Activity ◽  
The Common ◽  
High Peroxide

Experimental data concerning complex study of antioxidant activity of Y. pestis with different plasmid spectrum (wild-type Yersinia pestis subsp. pestis, Yersinia pestis subsp. altaica and their isogenic variants) are represented in the article. Superoxide dismutase activity of the tested Y.pestis strains was from 6.0 to 9.0×109 microbe cells. Signifi-cant differences between the Y.pestis strains with different plasmid composition were not detected by this parameter. Our results, consistent with the data of other authors, tell that superoxide dismutase activity is a thermo-inducible feature and does nоt depend on a plasmid spectrum. High peroxide destroying activity was also detected in all tested Y.pestis strains. The differences between the strains regarding their common peroxide destroying activity were found. This parameter of the plague microbe strains lacking pYP plasmid was at least 3times lower than common peroxide destroying activity in strains with this plasmid in the genome. In our opinion these revealed differences were caused by characteristics of plasmid spectrum. The common peroxide destroying activity’s degree of Y.pestis strains can be associated with the presence of pYP plasmid in the genome. The isogenic variants of Y.pestis strains lacking one of the plasmids had smaller pathogenic activity. This fact points to the need for further study of these strains.

scholarly journals Antioxidant system blood enzymes activity in patients with echinococcosis

2014 ◽  
Vol 95 (4) ◽  
pp. 531-533 ◽  
Author(s):  
N A Maslennikova ◽  
E Yu Sergeeva ◽  
E P Tikhonova ◽  
A Yu Burakov ◽  
N M Titova ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Catalase Activity ◽  
Treatment Options ◽  
The Body ◽  
Superoxide Dismutase Activity ◽  
Control Group ◽  
Lipid Peroxidation Product ◽  
Gradual Development ◽  
Peroxidation Product ◽  
New Treatment

Aim. To study the activity of antioxidant enzymes and concentration of malondialdehyde - lipid peroxidation product - in patients with echinococcosis. Methods. 22 patients aged 32 to 74 years [females - 9 (40%), males - 13 (60%), mean age 37.6 years] with established diagnosis of liver echinococcosis confirmed by ELISA, were examined. Mean disease duration was 10 years. 12 patients, in whom the diagnosis of liver echinococcosis was excluded, were included in the control group. Superoxide dismutase activity was determined based on adrenaline auto-oxidation inhibition, as well as catalase activity and malondialdehyde level were determined. Results. Statistically significant decrease in the malondialdehyde concentration from 5.9 [5.6÷6.6] to 3.7 [3.3÷4.1] mmol/g of hemoglobin (p 0.001), superoxide dismutase activity - from 764 [744÷796] to 666 [627÷695] i.u./min.×g of hemoglobin (p 0.001) was revealed in patients with echinococcosis. Catalase activity did not change significantly (maintained at the level of control group), which may indicate a temporary activation of the compensatory abilities of the body. Conclusion. Revealed data suggest that gradual development of oxidative stress and decrease in body adaptive abilities play a significant role in the pathogenesis of liver echinococcosis which should be considered while working out the new treatment options for the disease.

NGF restores decrease in catalase activity and increases superoxide dismutase and glutathione peroxidase activity in the brain of aged rats

1992 ◽  
Vol 12 (3) ◽  
pp. 177-181 ◽  
Author(s):  
Giuseppe Nisticò ◽  
Maria Rosa Ciriolo ◽  
Kayahan Fiskin ◽  
Michelangelo Iannone ◽  
Angelo de Martino ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Catalase Activity ◽  
Aged Rats ◽  
Glutathione Peroxidase Activity ◽  
The Brain
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