LIPID PEROXIDATION OF THE LIVER IN THE CONDITIONS OF HEAT EXPOSURE AND INTRODUCTION OF PHYTOADAPTOGENS

2016 ◽  
Vol 1 (62) ◽  
pp. 76-80
Author(s):  
Елена Юртаева ◽  
Elena Yurtaeva ◽  
Раиса Анохина ◽  
Raisa Anokhina ◽  
Наталья Симонова ◽  
...  
Keyword(s):  
Antioxidant System ◽  
Heat Exposure ◽  
Malonic Dialdehyde ◽  
The Body ◽  
Control Group ◽  
Peroxidation Of Lipids ◽  
Lipid Hydroperoxides ◽  
Experimental Conditions ◽  
Diene Conjugates ◽  
System Activity

Modern environmental conditions dramatically increased the level of radical forming processes in the body. Heat exposure stimulates the generation of reactive oxygen species inducing peroxidation of lipids as a result of the development of hypoxia. In experimental conditions the possibility to correct free radical lipid oxidation of rats’ organism membranes was studied with the oral introduction of the phytoadaptogens that contain the complex of natural antioxidants. The animals were divided into 5 groups and each of them had 30 rats: intact animals (1) which were held in standard conditions of vivarium; the control group (2) in which rats were exposed to heat during forty-five minutes daily; the experimental groups (3, 4, 5) in which before the effects of heat animals had a daily oral intake of the extracts of Eleutherococcus, Rhodiola, liquorice in a dose of 1 ml/kg. It was found out that in the liver tissue of experimental animals a daily heat exposure during forty-five minutes contributes to the increase of lipid hydroperoxides level (by 34-41%), of diene conjugate (by 45-50%), and of malonic dialdehyde (by 62-74%) against the decrease of antioxidant system activity in the liver of intact animals. The introduction of phytoadaptogens to rats in the conditions of heat exposure contributes to the reliable decrease in the liver of lipid hydroperoxides by 22-28%, of diene conjugates by 22-32%, and of malonic dialdehyde by 30-37% in comparison with the rats of the control group. While analyzing the effect of the phytoadaptogens on the activity of the components of antioxidant system it was shown that the level of ceruloplasmin in the liver of animals was reliably higher by 68-82%, of vitamin E by 42-65% in comparison with the same parameters of the rats of the control group. So, the application of phytoadaptogens in the conditions of long heat exposure of the organism of animals under experiment leads to the stabilization of the processes of peroxidation against the increase of antioxidant system activity.

scholarly journals COMPARATIVE EFFECTIVENESS OF SUCCINIC ACID AND REAMBERIN IN THE OXIDATIVE STRESS IN EXPERIMENT

2018 ◽  
Vol 1 (70) ◽  
pp. 78-82 ◽  
Author(s):  
Наталья Симонова ◽  
Natalya Simonova ◽  
Владимир Доровских ◽  
Vladimir Dorovskikh ◽  
А Кропотов ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ultraviolet Radiation ◽  
Succinic Acid ◽  
Antioxidant System ◽  
Malonic Dialdehyde ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Diene Conjugates ◽  
System Activity ◽  
Experimental Group

The search and development of methods for correction of oxidative stress in conditions of exposure to adverse environmental factors is an actual problem of modern medicine. In experimental conditions the possibility to correct free radical lipid oxidation of rats’ organism membranes was studied with the introduction of the succinic acid and of the succinate containing medication called Reamberin (Polysan, St. Petersburg). The animals were divided into 4 groups and each of them had 20 rats: the group with intact animals which were held in standard conditions of vivarium; the control group in which rats were exposed to ultraviolet radiation during three minutes daily; the experimental group in which before ultraviolet radiation animals had a daily intraabdominal intake of the succinic acid in a dose of 100 mg/kg; the experimental group in which before ultraviolet radiation animals had a daily intraabdominal intake of Reamberin in a dose of 100 mg/kg (20 ml/kg). It was found out that in the blood of experimental animals a daily ultraviolet radiation during three minutes contributes to the increase of lipid hydroperoxides level (by 48-53%), of diene conjugate (by 43-48%), and of malonic dialdehyde (by 48-61%) against the decrease of antioxidant system activity in the blood of intact animals. The introduction of the succinic acid to rats contributes to the significant decrease in the blood of lipid hydroperoxides by 15-16%, of diene conjugates by 9-16%, and of malonic dialdehyde by 15% in comparison with the rats of the control group. The introduction of the succinate containing Reamberin to rats in the conditions of oxidative stress contributes to the significant decrease of lipid hydroperoxides in the blood by 27-28%, of diene conjugates by 23-28%, and of malonic dialdehyde by 26-29% in comparison with the rats of the control group. While analyzing the effect of the succinate containing drugs on the activity of the components of antioxidant system it was shown that the level of ceruloplasmin in the blood of animals was significantly higher by 25-32%, of vitamin E by 28-33% in comparison with the rats of the control group. So, the application of the succinate containing antioxidants in the conditions of ultraviolet radiation under experiment leads to the stabilization of the processes of peroxidation against the increase of antioxidant system activity. The intraabdominal introduction in laboratory animals of Reamberin in a dose of 100 mg/kg of succinate prevents the accumulation of lipoperoxidation products and increases the activity of main components of the antioxidant system in rats’ blood plasma, which indirectly exceeds similar effect of succinic acid in a dose of 100 mg/kg in the conditions of oxidative stress.

scholarly journals COMPARATIVE EFFECTIVENESS OF SYNTHETIC AND NATURAL ANTIOXIDANTS IN TOXIC LIVER DAMAGE BY CARBON TETRACHLORIDE

2018 ◽  
Vol 1 (67) ◽  
pp. 64-69 ◽  
Author(s):  
Наталья Симонова ◽  
Natalya Simonova ◽  
Владимир Доровских ◽  
Vladimir Dorovskikh ◽  
Михаил Штарберг ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Antioxidant System ◽  
Liver Damage ◽  
Malonic Dialdehyde ◽  
Natural Antioxidants ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Diene Conjugates ◽  
Toxic Liver Damage ◽  
Experimental Group

Currently there is an active search for medicines increasing the stability of liver to pathological effects, reinforcing its neutralizing function, promoting its functional recovery after various injuries, including poisoning by industrial poisons. Therefore, the study of opportunities to reduce toxic liver damage by carbon tetrachloride introduction of synthetic and natural antioxidants is of interest. In experimental conditions the possibility to correct free radical lipid oxidation of rats’ organism membranes was studied with the introduction of the succinate containing drug called Reamberin (Polysan, St.Petersburg) and licorice syrup. The animals were divided into 4 groups and each of them had 10 rats: the group with intact animals which were held in standard conditions of vivarium; the control group in which rats were given carbon tetrachloride during 3 days daily; the experimental group in which before the introduction of carbon tetrachloride animals had a daily intra-abdominal intake of the Reamberin in a dose of 100 mg/kg (20 ml/kg); the experimental group in which before the introduction of carbon tetrachloride animals had a daily oral intake of the licorice syrup in a dose of 5 ml/kg. It was found out that in the blood and in the liver of experimental animals an introduction of carbon tetrachloride during 3 days contributes to the increase of lipid hydroperoxides level (by 24-43%), of diene conjugate (by 19-47%), and of malonic dialdehyde (by 61-81%) against the decrease of antioxidant system activity in the blood of intact animals. The introduction of the succinate containing drug to rats in the conditions of oxidative stress contributes to the decrease in the blood and in the liver of lipid hydroperoxides by 13-26%, of diene conjugates by 11-28%, and of malonic dialdehyde by 30-34% in comparison with the rats of the control group. Adding of the licorice syrup to rats contributes to the reliable decrease in the blood and in the liver of lipid hydroperoxides by 6-23%, of diene conjugates by 9-24%, and of malonic dialdehyde by 25-30% in comparison with the rats of the control group. While analyzing the effect of the antioxidants on the activity of the components of antioxidant system it was shown that the level of ceruloplasmin in the blood and in the liver of animals was reliably higher by 10-47%, of vitamin E by 13-42% in comparison with the same parameters of the rats of the control group. So, the application of the synthetic and natural antioxidants in the conditions of introduction of carbon tetrachloride of the organism of animals under experiment leads to the stabilization of the processes of peroxidation against the increase of antioxidant system activity.

scholarly journals ANTIOXIDANT PROPERTIES OF CONVOLVULUS ARVENSIS AND STELLARIA MEDIA IN THE CONDITIONS OF ULTRAVIOLET RADIATION

2017 ◽  
Vol 1 (65) ◽  
pp. 94-98
Author(s):  
Елена Юртаева ◽  
Elena Yurtaeva ◽  
Владимир Доровских ◽  
Vladimir Dorovskikh ◽  
Наталья Симонова ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ultraviolet Radiation ◽  
Antioxidant System ◽  
Malonic Dialdehyde ◽  
Ultraviolet Rays ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
External Application ◽  
System Activity ◽  
Experimental Group

The search and development of methods for correction of oxidative stress in conditions of exposure to adverse environmental factors is an actual problem of modern medicine. In experimental conditions the possibility to correct free radical lipid oxidation of rats’ cell membranes was studied with the external application of the ointment of herb convolvulus and of the ointment of herb chickweed. The animals were divided into 4 groups and each of them had 20 rats: intact animals which were held in standard conditions of vivarium; the control group in which rats were exposed to ultraviolet radiation during three minutes daily; the experimental group in which before ultraviolet radiation animals had a daily external application of the ointment of herb convolvulus; the experimental group in which before ultraviolet radiation animals had a daily external application of the ointment of herb chickweed. It was found out that in the blood of experimental animals a daily ultraviolet radiation during three minutes contributes to the increase of lipid hydroperoxides level (by 15-19%), of diene conjugate (by 14-16%), and of malonic dialdehyde (by 40-66%) against the decrease of antioxidant system activity in the blood of intact animals. The introduction of the ointment of herb convolvulus and of the ointment of herb chickweed to rats in the conditions of oxidative stress contributes to the reliable decrease in the blood of lipid hydroperoxides by 9-13%, of diene conjugates by 8-13%, malonic dialdehyde by 15-28% in comparison with the rats of the control group. While analyzing the effect of the ointment on the activity of the components of antioxidant system it was shown that the level of ceruloplasmin in the blood of animals was significantly higher by 13-20%, of vitamin E by 8-14%, of catalase by 13-28% in comparison with the same parameter of the rats of the control group. So, the application of the ointment of herb convolvulus and of the ointment of herb chickweed in the conditions of oxidative stress induced by the influence of ultraviolet rays leads to the stabilization of the processes of peroxidation against the increase of antioxidant system activity.

scholarly journals Correction of antioxidant protection of cows organism in theperiod of laboratory drugs with content of non-participants of metals

2018 ◽  
Vol 20 (83) ◽  
pp. 204-207
Author(s):  
I.B. Kobilyukh
Keyword(s):  
Antioxidant System ◽  
Free Radical Oxidation ◽  
The Body ◽  
Control Group ◽  
Antioxidant Protection ◽  
Dry Period ◽  
Diene Conjugates ◽  
Radical Oxidation ◽  
Black And White ◽  
White Breed

The enhancement of free radical oxidation, characteristic of inflammatory processes, is accompanied by a violation of the properties of biological membranes, the functioning of cells and damage to surrounding tissues. The purpose and task of the research was to make correction of antioxidant protection of the body of cows during the period of dry preparations containing nanoparticles of metals. To activate the antioxidant system of the body of cows during the dry period, use the vitamin complex «Nanovit». The experiment was conducted on cows of Ukrainian dairy black-and-white breed in Agroprodservice-Invest LLP of the Kozivsky District of the Ternopil Oblast. The experiment was conducted before 35–25 days to the calves birth where the experimental and control groups of cows were formed (n = 10). Cows of the experimental group for 35–25 days to the cattle, intramuscularly injected 5.0 ml vitamin complex «Nanovit» (composition: vitamins A, D, E and nanoparticles Cu, Zn, Mn, Co). Producer of «NVP» LLC «Ecological Capital». Cows of the control group for 35–25 days to the catheter, intramuscularly administered 15.0 ml of the drug Prodevit-tetra (1 ml of the preparation contains vitamin A – 50000 IU, vitamin D – 25000 IU, vitamin E – 20 mg, vitamin F – 6 mg). Manufacturer of Product Ltd. The study found that vitamins A, D, E, and nanoparticles Cu, Zn, Mn, Co, which are part of the drug «Nanovit», activate the antioxidant system of the body of cows during the dry period. So after the application of the drug «Nanovit» in the body of cows there was a decrease of 58.5% (Р ≤ 0.01) of diene conjugates and 95.0% (Р ≤ 0.001) of TBK-active products against the background of an increase of 20.0% (Р ≤ 0.01) activity of catalase. After the application of the drug «Nanovit», the service period of the period from cows decreased by 54 days, and the index of insemination decreased by 0.2 units against the cows fed «Prodevit-tetra», indicating a positive effect of the drug containing the nanoparticles of metals Cu, Zn, Mn, Co and vitamins A, D, E during the post-period period in cows.

scholarly journals Про-антиокcидантна рівновага в печінці та м’язах cтерляді за дії штучного гіпобіозу й анестезії

2017 ◽  
Vol 7 (3) ◽  
pp. 43-49
Author(s):  
V. V. Gryshchenko ◽  
N. Vovk ◽  
О. Shlapak
Keyword(s):  
Carbon Dioxide ◽  
Lipid Peroxidation ◽  
Malonic Dialdehyde ◽  
Thiobarbituric Acid ◽  
The Body ◽  
Stress Factors ◽  
Control Group ◽  
Experimental Conditions ◽  
Colored Complex ◽  
Group I

<p>The pro-antioxidant balance in the liver and muscles of sterlet under the influence of artificial carbonic dioxyde hibernation and anaesthesia have been studied. In experiments were used two-years old sterlet weighing 170–210 g which were grown under conditions of a closed water supply. Four groups of five copies of each fish were formed: Control group I (fish remained intact); Group II (the clove oil was used for fish anaesthesia, which was added to the water); Group III (fish were hibernated by carbon dioxide); Group IV - control of hibernation (after complete recovery from carbon dioxide hibernation, these groups of fish were returned to the pool of incubation shop for her follow–up). The research of intensity of the formation products of peroxide oxidation of lipids (POL) in the body of sterlet in the experimental conditions were conducted by determining the content of thiobarbituric acid active products (TBA–active products) in the muscles and liver that are generated at the final stages of lipid peroxidation. The content of malonic dialdehyde (MDA), one of the intermediate products of lipid peroxidation of membrane was determined photometrically by the concentration of colored complex formed by its reaction in the acidic environment of the two molecules of thiobarbituric acid (TBA). Catalase activity was determined by the ability of hydrogen peroxide to form the stable colored complex with Molybdenum salts. The results support the adaptive character in fluctuations in the antioxidant protection system by the actions of carbon dioxide hibernation and anaesthesia on the sterlet body. This also contributes to usage of these conditions in fish transportation at long distances without stress factors.</p>

scholarly journals Prooxidant-antioxidant balance in the organism of bulls (young cattle) after using cadmium load

2017 ◽  
Vol 7 (4) ◽  
pp. 589-596 ◽  
Author(s):  
B. Gutyj ◽  
V. Stybel ◽  
L. Darmohray ◽  
Y. Lavryshyn ◽  
I. Turko ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Enzyme Level ◽  
Malonic Dialdehyde ◽  
Control Group ◽  
Peroxidation Of Lipids ◽  
Antioxidant Protection ◽  
Diene Conjugates ◽  
Young Cattle ◽  
Antioxidant Protection System

<p>The results of studies of the effect of Cadmium influence on the state of the enzyme and nonenzyme system of antioxidant protection of young cattle organism, namely on the activity of superoxide dismutase, catalase, glutathione peroxidase, level of reduced glutathione, Selenium, vitamins A and E. It was determined that the feeding of young bulls with cadmium chloride in a dose of 0.04 mg / kg of body weight contributed to a decrease both, enzyme and non enzyme level of the system of antioxidant protection (superoxide dismutase by 22%, catalase - by 12%, renewed glutathione by 11%, glutathione peroxidase by 22 %, Selenium - by 18%, vitamin A - by 23%, vitamin E - by 27%,). The lowest level of the antioxidant protection system in the blood of young cattle was set at the sixteenth and twenty-fourth day of the experiment, which is due to the increased activation of lipoperoxidation processes and a disturbance of the balance between the activity of the antioxidant system and the intensity of peroxidation of lipids. The toxic effect of Cadmium contributes to the change in the stationary concentrations of the radical metabolites O2, ˙OH, NO2, which, in their turn, initiate the processes of peroxide oxidation of lipids, indicating an increase in the level of diene conjugates and malonic dialdehyde. The highest level of intermediate and final products of peroxid oxidation of lipids in the blood of young cattle was determined on the thirtieth day of the experiment, while comparing with the control group, they increased by 31 and 27%, respectively.</p>

scholarly journals Assessment of the state of lipid peroxidation and antioxidant system in children with nephrotic form of glomerulonephritis

2017 ◽  
Vol 98 (3) ◽  
pp. 370-375
Author(s):  
R O Beglyarov
Keyword(s):  
Lipid Peroxidation ◽  
Nephrotic Syndrome ◽  
Blood Plasma ◽  
Antioxidant System ◽  
Malonic Dialdehyde ◽  
Control Group ◽  
Chronic Glomerulonephritis ◽  
Diene Conjugates ◽  
Control Value ◽  
In Blood Plasma

Aim. Study of the activity of lipid peroxidation and state of antioxidant system in children with a nephrotic form of chronic glomerulonephritis. Methods. 104 children with a nephrotic form of glomerulonephritis were examined. The average age of children was 10.18±4.03 years. 46.2% of children were in remission, 32.7% of children had 1st degree of activity, 14.4% had 2nd degree, and 6.7% had 3d degree of nephrotic syndrome. Control group included 30 children without chronic glomerulonephritis. The levels of diene conjugates, malonic dialdehyde, catalase, reduced glutathione, glutathione peroxidase, and glutathione reductase in blood plasma and erythrocytes were determined. Results. In children with chronic glomerulonephritis the concentration of diene conjugates and malonic dialdehyde was elevated in blood plasma and erythrocytes. In erythrocytes their concentration was statistically significantly higher at all degrees of activity of nephrotic syndrome than in control group. The level of catalase in patients’ blood plasma in comparison with the control value was reduced by 16.6%, in erythrocyte mass - by 25.9% (p

scholarly journals The state of antioxidant protection system in cows under the influence of heavy metals

2020 ◽  
Vol 11 (2) ◽  
pp. 237-242
Author(s):  
L. G. Slivinska ◽  
A. R. Shcherbatyy ◽  
B. O. Lukashchuk ◽  
B. V. Gutyj
Keyword(s):  
Heavy Metals ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Malonic Dialdehyde ◽  
Protection System ◽  
Lipid Hydroperoxides ◽  
Technogenic Pollution ◽  
Antioxidant Protection ◽  
Diene Conjugates ◽  
Antioxidant Protection System

A highly relevant problem of modern veterinary science is the study of features and mechanisms of combined action of the most common heavy metals – cadmium and plumbum and their influence on the body of humans and animals in the regions of Ukraine under technogenic pollution. The purpose of the work was to study the influence of heavy metals on the state of the antioxidant protection system of cows, in particular on the content of lipid peroxidation products (malonic dialdehyde, lipid hydroperoxides and diene conjugates), and activity of antioxidant enzymes (glutathione peroxidase and superoxide dismutase), depending on the distance to the heaps of mines in the coal basin. The study objects were cows of black-and-white breed at the age of 3–7 years. It was established that this parameter in the place with the highest concentration of diene conjugates in the blood of cows was by 25.8 % higher compared to the place of low concentration and 12.1 % higher compared to the place with medium concentration. In the place with the highest content of lipid hydroperoxides in the blood of cows the parameter was 23.7 % higher compared to the cows from the place with the low content. The concentration of lipid hydroperoxides in the blood of cows from the place with the medium content was 16.7% higher compared to the cows from the place with the low content. The parameter from the place with the lowest content of lipid hydroperoxides in the blood of cows was 12.1% lower compared to the place with the highest content. The level of malonic dialdehyde in the blood of cows from the technogenic pollution zone in the place with the largest amount was higher by 36.2; 34.0 and 18.8 % – compared to places with medium and low levels, respectively. The activity of superoxide dismutase in the blood of cows in the place with its highest activity was 0.284 ± 0.0099 % block. reac/g Hb, and in the place with the lowest activity – 0.23 ± 0.0051 % block. reac/g Hb. The activity of glutathione peroxidase in the blood of cows in farms of the technogenic pollution zone depended on the distance to the mine. These researches will further develop effective methods of treating cows under the influence of heavy metals, in particular regarding the antioxidant system.

scholarly journals The Clinical and Hematological changes in Rabbits exposed to Melia azedarach fruits under experimental conditions

2015 ◽  
Vol 1 (1) ◽  
pp. 12 ◽  
Author(s):  
AL- Zubaedi Raad Mahmood
Keyword(s):  
Body Weight ◽  
Body Temperature ◽  
Bleeding Time ◽  
Hematological Parameters ◽  
The Body ◽  
Melia Azedarach ◽  
Control Group ◽  
Clotting Time ◽  
Experimental Conditions ◽  
Hb Concentration

The study was conducted on 10 local breed rabbits, of 1-2 years age, of 1- 1.5 kg body weight. They were feed concentrated and green food, and left ad libitum for water, and kept in room of 20- 25 oC. The animals divided into two groups of 5 each. First as treated group received the plant in powder form mixed with the food at a dose rate of 5 g/ animal / day for three weeks , while the second left without exposure as a control group .The main dependent parameters in the study were , clinical parameters ( body temperature , heartbeat , respiratory rate, body weight , in addition to monitor any abnormal signs appear on the animals . While the main hematological parameters were included , RBC count , WBC count , Hb concentration,PCV percentage , Red cell indices , Bleeding time and Clotting time. The body temperature, respiratory rates, heart rate were increased .The body weight decreased .The bleeding time and clotting time were prolonged. The erythrocyte count, Hb concentration, and MCV values were decreased; PCV and MCHC did not showed any significant changes. The MCH increased. The total leucocytes count and the basophiles percentage did not showed any significant changes. The Heterophils and monocytes percentage increased .The lymphocytic and Eosinophil percentage were decreased during the study.

scholarly journals The Assessment of the Integrated Antioxidant System of the Body in the Course of Radon Therapy: A Pilot Study

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Jadwiga Kuciel-Lewandowska ◽  
Jan Gnus ◽  
Lilla Pawlik-Sobecka ◽  
Sylwia Płaczkowska ◽  
Izabela Kokot ◽  
...  
Keyword(s):  
Antioxidant System ◽  
Colorimetric Assay ◽  
Total Antioxidant Status ◽  
The Body ◽  
Control Group ◽  
Study Group ◽  
Disc Disease ◽  
Total Antioxidant ◽  
Radon Water ◽  
And Control

Introduction. The sources of Reactive Oxidative Species (ROS) in the organism are the respiratory processes occurring in cells catalyzed by different enzymes. Operation of ROS is balanced by antioxidants, the compounds; although present in low concentrations, they significantly inhibit the degree of oxidation of particular molecules. The Aim of the Study. The aim of this study was to assess the changes in the integrated antioxidant system under the influence of radon therapy in osteoarthritis patients. Material and Methods. Observation included 35 patients suffering from degenerative joints and disc disease (mean age 56.5 years) undergoing radon water therapy and control group that consisted of 15 osteoarthritis patients (mean age 54.2) without contact with radon water. Before therapy and after 18 days of treatment, serum total antioxidant status (TAS) was assessed with the use of standard colorimetric assay. Results. In the study group, we observed trends to increase TAS concentration, whereas, in the control group, TAS concentration was decreasing. Conclusions. (1) Radon waters treatment influenced the level of TAS of osteoarthritis patients treated with the radon water. (2) The change in TAS concentrations in the study group may be the result of low doses of ionizing radiation, but further studies on larger patient’s groups are demanded. This study is registered with number NCT03274128.

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