DYNAMICS OF PROTEIN METABOLISM AND ACTIVITY OF AMINOTRANSFERASES OF DOGS BY ADDING DIHYDROQUERCETIN

2016 ◽  
Vol 1 (2) ◽  
pp. 60-63
Author(s):  
Полищук ◽  
Sergey Polishchuk ◽  
Молянова ◽  
Galina Molyanova
Keyword(s):  
Body Weight ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
The Body ◽  
German Shepherd ◽  
Physiological Indicators ◽  
Formalin Edema ◽  
And Function ◽  
Experimental Group

The purpose of research is to increase protective and adaptive reactions, the service and the working potential of an organism of dogs through the use of DHQ. The article presents the results of an experiment to the effect of DHQ on the dynamics of hematological parameters of dogs blood. Dihydroquercetin – an active antioxidant, a natural scavenger of oxygen free radicals, hepatoprotective, has anti-inflammatory action due to limitations of the formalin edema and histamine, and inhibits the formation of serous fluid, pain killers, immunocorrectional properties. Due to the high complexing properties it displays the body of heavy metals, including radionuclides, it helps to restore the tone of blood vessels, normalization of the lipid spectrum of the blood and slows the development of atherosclerotic plaques. Investigations were carried out in Dogs State Service Ministry of I A of Russia zonal center in Samara Region by clinically healthy of dog breed German Shepherd, age 2-4 years with an average body weight of 30 kg on the background conditions and feeding, adopted by the enterprise. Dogs experimental group received Dihydroquercetin to 0.001 g/kg body weight dose once a day with food. When added to the basic diet Dihydroquercetin dog experimental group show an increase in indicators such as red blood cells – by 18.3% (p<0.01), hemoglobin – by 11.7% (p <0.01) hematocrit – 7.1% (p<0.01), white blood cells – by 9.1% (p<0.05) compared to control data. The research results give reason to believe that the use of dietary supplements in Dihydroquercetin in dogs diet dose 0.001 g/kg allows significantly increase the morphological and physiological indicators of animal organism. In this case a significant increase erythrocytes and hemoglobin and hematocrit indicates increased oxidative metabolism and function of the intensity of the blood in dogs treated with the drug, and increase in the blood indicates an increase in immune status.

DYNAMICS OF PROTEIN METABOLISM AND ACTIVITY OF AMINOTRANSFERASES OF DOGS BY ADDING DIHYDROQUERCETIN

2016 ◽  
Vol 1 (1) ◽  
pp. 6-8
Author(s):  
Полищук ◽  
Sergey Polishchuk ◽  
Молянова ◽  
Galina Molyanova
Keyword(s):  
Body Weight ◽  
The Body ◽  
Control Group ◽  
German Shepherd ◽  
Formalin Edema ◽  
Working Dogs ◽  
Healthy Dogs ◽  
Biochemical Indices ◽  
Experimental Group ◽  
Operational Capacity

The purpose of research is to improve business and operational capacity of the body through the use of dogs dihydroquercetin. The article presents the results of experiment by the effect of dihydroquercetin dynamics of biochemical indices of the dogs blood. Dihydroquercetin – it is an active antioxidant, natural scavenger of oxygen free radicals, hepatoprotector, having anti-inflammatory action due to limitations of the formalin edema and histamine, and inhibits the formation of serous fluid, painkillers, immunocorrelation properties. Due to the high complexing properties it displays the body of heavy metals, including radionuclides, helps to restore the blood vessels tone, normalizing the lipid levels and slows the progression of atherosclerotic plaques. Investigations were carried out in zonal center of the dog expert service GU MVD of Russia in Samara region with clinically healthy dogs, German shepherd breed, age 2-4 years with an average body weight of 30 kg in the background conditions and feeding, adopted by the enterprise. The dogs of the experimental group received dose dihydroquercetin 0.001 g/kg for body weight once a day during meals. When added to the basic diet, dihydroquercetined dogs of the experimental group shown the increase in indicators such as: total protein – by 11.5% (p<0.01), albumin – to 12.8% (p<0.01), AST 13.6% (p <0.001), ALT – by 11% (p<0.05), alkaline phosphatase in the – 12% (p<0.01) relative to the control group. Based on the results, obtained during the experiment, we can conclude that the use of dietary supplements in the diet of dogs, dihydroquercetin allows biocorrection level of protein and enzymatic metabolism in the body, which improves service and working dogs potential.

scholarly journals Hematological and histological effect of fractionated neem leaf extract in healthy Wistar rats

2020 ◽  
Vol 24 (4) ◽  
pp. 314-321
Author(s):  
David Chibuike Ikwuka ◽  
◽  
Ed Nwobodo ◽  
Bond U. Anyaehie ◽  
Emmanuel I Umegbolu ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Cells ◽  
Leaf Extract ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Central Vein ◽  
Prolonged Administration ◽  
Neem Extract ◽  
Neem Leaf Extract ◽  
Experimental Group

Introduction: In recent years, the growing research towards new drugs has been targeted on plant-based drugs, and Neem (Azadirachta indica) is one of the plants that have been extensively researched for its diverse medicinal properties. The study aimed to determine the effects of neem on the hematological parameters (total white blood cells, neutrophil, monocyte and eosinophil counts) and histology of some organs of rats. Methods: Fifteen healthy male Wister rats divided into control (Nm0) and experimental groups (Nm11 and Nm22). Control group 1 (Nm0) was given 100mg/200g body weight of normal saline orally twice daily; experimental group 2 (Nm11), 100mg/200g body weight neem extract twice daily for 11 days and experimental group 3 (Nm22), 100mg/200g neem leaf extract twice daily for 22 days. Total number of white blood cells (WBC), lymphocytes, neutrophils, monocytes and eosinophils, packed cell volumes (PCV) and histological changes in the spleen, liver and kidneys were evaluated. Results: There were no significant differences in mean values of the hematological parameters (total WBC; PCV; neutrophils, lymphocytes, monocytes and eosinophils). We observed the central vacuolation and accumulation of lymphocytes in the spleen, hypertrophy of the central vein in the liver and shrinking of the glomeruli and accumulation of the lymphocytes in the kidney using hematoxylin and eosin staining following prolonged administration of neem extract (Nm22). Conclusion: Prolonged administration of neem affected the histology of some organs of the rats more than the hematological parameters.

Evaluation of the effectiveness of ironcontaining drugs in the complex therapy of antenatal hypotrophy of piglets

2021 ◽  
Vol 1 (6) ◽  
pp. 13-17
Author(s):  
R. V. Rogov ◽  
◽  
Zh. Yu. Muradyan ◽  
I. G. Ryazanov ◽  
◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Clinical Study ◽  
Body Weight Gain ◽  
Hematological Parameters ◽  
Live Weight ◽  
The Body ◽  
Treatment Regimens ◽  
Complex Therapy ◽  
Experimental Group

In the conditions of the pig breeding complex, the effectiveness of two treatment regimens for antenatal hypotrophy of piglets was studied. Two groups of piglets were formed, where the ironcontaining drug «Ursoferran – 200» was used in complex therapy in the 1st experimental group, and «Ferropen-200»in the 2nd experimental group. In a clinical study, hypotrophic piglets showed characteristic symptoms of the disease. In the experiment, the live weight of piglets was measured at the time of farrowing and on the 3rd, 7th, 14th and 21st days of life. The hematological parameters were studied. The most pronounced therapeutic effect was observed in the 1st experimental group, where the body weight gain was higher by 8,3% than in the 2nd experimental group.

scholarly journals EFFECT OF DEUTERIUM WATER ON BLOOD VALUES AND DIGESTIBILITY OF NUTRIENTS OF RHESUS MACAQUE

2020 ◽  
Vol 384 (2) ◽  
pp. 22-28 ◽  
Author(s):  
N. V. Gaponov ◽  
S. V. Svistunov ◽  
N. N. Bondarenko ◽  
I. A. Romanenko
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
The Body ◽  
Control Group ◽  
Deuterium Content ◽  
Animal Blood ◽  
Constant Weight ◽  
Effect Of Water

The use of water with a modified isotopic composition in the diet of humans and animals, for example, with a reduced deuterium content, affects the rate of biological processes. In this regard, the targeted formation of the isotopic D / H gradient in the body can be used to increase its adaptive properties to external influences. Primates have a high anatomical and physiological similarity with humans, therefore, are an indispensable model for reproducing various pathological and toxic conditions in humans. The aim of the study was to study the effect of water with a low deuterium content on the hematological and biochemical blood parameters of rhesus monkeys and on the digestibility of nutrients of the diet. In the control group, primates consumed water with a natural regional content of deuterium equal to 150 mg / l, and in the experimental group - with reduced to 50 mg/l. The duration of the experiment was 35 days. The material for the study was venous blood, serum and feces of primates. Blood was drawn from monkeys before the use of deuterium depleted water and after the completion of the experiment. All blood samples (2.5-3.0 ml) were taken from the cubital or femoral veins of animals on an empty stomach and stabilized with a heparin solution. Laboratory studies of animal blood were performed on an automatic hematological analyzer (Beckman Coulter, USA) of the CoulterAcT 5diffCP brand. In order to study the effect of water with a reduced deuterium content on hematological parameters, we determined the number of red blood cells, white blood cells, platelets, hemoglobin concentration, hematocrit, the average volume of red blood cells, red blood cell anisocytosis, and erythrocyte sedimentation rate (ESR). The metabolism trial was divided into two periods. Preliminary - to exclude the influence of previous feeding and accustoming primates to cage conditions of keeping. Experimental - conducting a thorough accounting of consumed feed, excreted excrement. Feces were collected daily at the same time (morning and evening), weighed and ground in a mortar. At each collection, 50% of the homogenized mass was taken for analysis. The collected portions were stored in the refrigerator. After the end of the experimental (accounting) period in the collected feces, the initial moisture was determined by drying at 60-70 ℃ to constant weight. The resulting air-dry mass was thoroughly ground and analyzed. It has been found that the introduction of water with a reduced deuterium content into the diet of primates has a positive effect on feed conversion rate and blood biochemical parameters.

scholarly journals Sex-Related Differences in Hematological Parameters and Organosomatic Indices of Oreochromis niloticus Exposed to Aflatoxin B1 Diet

Scientifica ◽  
2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Esther Marijani ◽  
Johnson Nasimolo ◽  
Emmanuel Kigadye ◽  
Gbemenou Joselin Benoit Gnonlonfin ◽  
Sheila Okoth
Keyword(s):  
Body Weight ◽  
Oreochromis Niloticus ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Control Group ◽  
Feeding Experiment ◽  
Initial Body Weight ◽  
Male And Female ◽  
Males And Females

A 24-week feeding experiment was conducted to assess whether males and females of Oreochromis niloticus exhibit differences in their hematological responses and organosomatic indices to dietary AFB1 contamination. Triplicate groups of O. niloticus (initial body weight: 24.1 ± 0.6 g) were fed with four diets (Diets 1 to 4) containing 0, 20, 200, and 2,000 μg AFB1 kg−1. A significant decrease (P<0.05) in hemoglobin (Hb), red blood cells (RBC), and hematocrit (Hct) was observed in AFB1 exposure groups, with the lowest levels recorded in the 2000 μg AFB1 kg−1 treatment. A significant increase in mean white blood cells (WBC), neutrophils, and lymphocytes was observed in AFB1 exposure groups. No sex-related differences in RBC, WBC, lymphocytes, monocytes, and neutrophils levels were observed. However, hemoglobin and hematocrit values for female O. niloticus were significantly lower than those for male O. niloticus. Organosomatic indices showed that the relative liver, kidney, and spleen weights were significantly higher (P<0.05) in the AFB1 supplemented group than in the control group. However, the effect of aflatoxin on organosomatic indices does not depend on sex but rather depends on the dose of aflatoxin in the diet. These results provide useful information for monitoring changes in the health status of male and female O. niloticus.

scholarly journals Evaluation de la toxicité d’un aphrodisiaque ivoirien d’origine naturelle (aphro) chez le rat

2021 ◽  
Vol 15 (4) ◽  
pp. 1595-1604
Author(s):  
Moussa Gbogbo ◽  
Rufin Yao Kouadio ◽  
Félicité Tano-bla Aboli ◽  
Mama Kone ◽  
Elisée Kouassi Kporou ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Cells ◽  
Blood Platelets ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Wistar Rat ◽  
Control Group ◽  
World Population ◽  
Poids Corporel ◽  
Rate Of Increase

L’usage des plantes à des fins thérapeutiques est très ancien. De nos jours encore, elles sont utilisées par une frange partie de la population mondiale. La présente étude avait pour objectif d’étudier les effets toxiques de l'administration subaiguë d'un phytomédicament ivoirien Aphro vendu sur les marchés chez le rat Wistar albinos. Pour ce faire, le phytomédicament Aphro a été administré quotidiennement par voie orale à trois lots de rats, aux doses de 5,75 ; 11,5 et 23 mg/kg de poids corporel et cela pendant 28 jours. Le taux de croissance des rats, les paramètres hématologiques et biochimiques sanguins ont été étudiés. Les résultats de cette étude ont révélé qu’aux doses de 5,75 ; 11,5 et 23 mg/kg de poids corporel, le phytomédicament ne modifie pas de façon significative le taux d’accroissement de la masse corporelle et les paramètres hématologiques constitués du taux de globules rouges, de globules blancs, de plaquettes sanguines et le taux d’hémoglobine. Par ailleurs, des marqueurs hépatiques et lipidiques sériques des rats traités n'ont pas été perturbés par l’administration du phytomédicament comparativement au lot témoin. En revanche, la créatinine, l’un des marqueurs rénaux étudiés a connu une augmentation significative (p < 0,05) chez les rats traités avec le phytomédicament aux doses de 11,5 et 23 mg/kg de poids corporel par rapport au lot témoin. Cette étude a permis de montrer que l’usage prolongé et à doses élevées de Aphro pourrait entraîner des effets nocifs sur les reins.   English title: Assessment of the toxicity of an ivoirian natural aphrodisiac (aphro) on rat The use of plants for therapeutic purposes is very old. Even today, they are used by a part of the world population. The objective of the present study was to evaluate the toxic effects of the subacute administration of an ivorian phytomedicine Aphro sold in the markets in the albino Wistar rat. To do this, the phytomedicine Aphro was administered orally daily to three batches of rats, at doses of 5.75; 11.5 and 23 mg/kg of body weight for 28 days. Rats' growth rate, blood haematological and biochemical parameters were studied. The results of this study showed that at doses of 5.75; 11.5 and 23 mg/kg of body weight, the phytomedicine does not significantly modify the rate of increase in body mass and the hematological parameters consisting of the level of red blood cells, white blood cells, blood platelets and the level hemoglobin. Furthermore, hepatic and serum lipid markers of the treated rats were not disturbed by the administration of the phytomedicine compared to the control group. In contrast, creatinine, one of the renal markers studied, experienced a significant increase (p <0.05) in rats treated with the phytomedicine at doses of 11.5 and 23 mg / kg of body weight compared to the batch witness. This study showed that prolonged use and in high doses of Aphro could cause harmful effects on the kidneys.

scholarly journals Influence of sulfur complex on hematological indicators of the rabits

2018 ◽  
Vol 20 (92) ◽  
pp. 203-208
Author(s):  
A. Z. Dychok-Niedzielska ◽  
Y. V. Lesyk
Keyword(s):  
Body Weight ◽  
Sodium Sulfate ◽  
Hematological Parameters ◽  
Experimental Period ◽  
The Body ◽  
Control Group ◽  
Free Access ◽  
Group A ◽  
Hematological Indicators ◽  
Experimental Group

The article presents results on the influence of different amounts of sulfur citrate, manufactured using nanotechnology and sodium sulfate in rabbit diet from 60 to 118 days of age on the hematological parameters of their organism. Studies have been conducted on 30 rabbits of the Hyla hybrid divided into six groups (control and five experimental), 6 animals in each. Animals were kept in regulated microclimate and illumination in mesh cages of 50×120×30 cm. Controlled rabbits fed without restriction a balanced granulated feed with free access to water. The young of the first (E-II), the second (E-II), the third (E-III) and the fourth (E-IV) experimental groups feed the diet of the control group and, during the day, dispensed sulfur citrate from the calculation of 2; 4; 8 and 12 mg S/kg body weight. Rabbits (E-V) of the experimental group fed the diet of the control group and set water with sodium sulfate (Na2SO4) in the amount of 40 mg S/kg body weight. In the preparatory period – 60 days and in the experimental period – at 91 and 118 days of life (31 and 58 days of delivery of supplements), blood samples were collected from the regional anterior vein of rabbits for hematological studies performed using an automatic hematologic analyzer (Orphee Mythic-18, Switzerland). Researches have shown that the administration of citrate sulfur in the amount of 8 mg S/kg of body weight was marked by a greater number of red blood cells and leukocytes, respectively, by 13.4 and 23.3% (P < 0.05) and with a higher level of HCT at 16.2% (P < 0.05), MCH, RDV and MCHC, respectively, 11.1; 14.5 and 2.6% (P < 0.05) on day 58 of the experiment compared with the control group. In the blood of animals in the 2nd experimental group, a higher concentration of hemoglobin (Р < 0.05) was observed at 31 days and a higher HCT (Р < 0.05) level was 58 days compared to control. Thus, the results of the study of the hematological parameters of the body of rabbits indicate their positive changes in the actions of the physiologically substantiated amount of organic sulfur, which contributed to the activation of metabolic processes.

HEMATOLOGICAL PARAMETERS PUREBRED AND CROSSBRED CALVES

2016 ◽  
Vol 1 (3) ◽  
pp. 45-48
Author(s):  
Исхаков ◽  
Rishat Iskhakov
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Physiological State ◽  
White Blood Cells ◽  
The Body ◽  
Adaptive Plasticity ◽  
Total Protein Content ◽  
Black And White

The purpose of research is scientifically and practically prove the possibility of increasing the level of meat productivity purebred for young calves at the crossing of Black-motley cows with bulls of the breed Obrac. Research objects are the bulls of Black-motley breed and their hybrids I generation with Obrac breed. For the experience 4 groups of animals were formed: I and III – Black and white breed, II and IV – gobies Оbrac crossbreeds ½ x ½ black-and-white. Bulls of groups III and IV in the 2 months of age were castrated by open way. All experimental animals identical conditions of detention and feed have been established. To monitor the physiological state of an organism of young animals in the winter and in the blood of the summer, blood was taken from the jugular vein before feeding and watering, measured hemoglobin, alkaline reserve, the number of white blood cells, red blood cells, serum – total protein, protein fractions, calcium, phosphorus, , the activity of AST and ALT. Deviations from the physiological norm morphological parameters of blood in the experimental groups of calves during the experiment was not set. Total protein content in serum was higher in summer compared to winter. Increasing the total protein content have purebred calves was 4.1 g/l (5.3%), crossbred steers 3.3 g/l (4.2%), respectively castrates at 4.2 g/l (5.6%) and 4.9 g/l (6.5%). There is a trend increase of enzyme activity in the summer, due to a more active course of metabolism in the body during this season of the year. Purebred and crossbred young are characterized by high adaptive plasticity, as evidenced by morphological and biochemical indices of blood. All quantitative and qualitative changes in the blood were largely seasonal in nature and due to the influence of environmental conditions. Thus all interior change indicators do not go beyond the limits of the physiological norm.

scholarly journals EFFECT OF SILYMARIN EXTRACTED FROM SILYBUM MARIANUM ON NICKEL HEMATOTOXICITY AND NEPHROTOXICITY IN MALE ALBINO WISTAR RATS

2017 ◽  
Vol 9 (8) ◽  
pp. 84
Author(s):  
Samira Bouhalit ◽  
Zine Kechrid ◽  
Abdelfattah Elfeki
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Blood Cells ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Nickel Sulfate ◽  
Enzymatic Antioxidants ◽  
Silybum Marianum ◽  
Renal Markers

Objective: The objective of this study was to investigate the effect of silymarin extract from Silybum marianum against nickel-induced alterations in haematological indices, kidney dysfunction and renal antioxidant defence system.Methods: Male albino Wistar rats were divided into four groups seven each. Control, silymarin, nickel and nickel plus silymarin. Silymarin was administrated orally (100 mg/kg b. wt) and nickel as nickel sulfate (NiSO4 6H20) was given intraperitoneally (20 mg/kg b. wt) at alternative days. The experiment continued for three consecutive weeks. Body weight was recorded regularly. After overnight fasting, animals were killed and serum creatinine, serum urea, serum uric acid, hematological parameters and renal antioxidant markers were determined.Results: The treatment with nickel led to a significant decrease in body weight with an increase in both absolute and relative kidney weights and a significant increase in renal markers, which confirmed by histopathological alteration. A microcytic anemia was also observed, which was manifested by a reduction of red blood cells count (RBC), hemoglobin (Hb) concentration, platelet counts (Plt), hematocrit and white blood cells counts (WBC). The level of lipid peroxidation was increased. Whereas, GSH concentration and enzymatic antioxidants SOD, GSH-Px and CAT activities were decreased. The co-treatment with methanolic extract of milk thistle attenuated the variation in the hematological and renal markers, decreasing renal lipid peroxidation (p<0.05) with a concomitant increasing reduced glutathione content (p<0.01) and restoring the antioxidant enzymes (SOD, CAT, GSH-Px) in kidney, as well as an improvement in histological changes compared to those previously noticed in nickel group.Conclusion: To conclude, these findings demonstrated that silymarin extract effectively improved heamatotoxicity and nephrotoxicity caused by nickel.

scholarly journals Comparative Effects of Using New Multi-Strain Synbiotics on Chicken Growth Performance, Hematology, Serum Biochemistry and Immunity

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1555
Author(s):  
Artur Żbikowski ◽  
Karol Pawłowski ◽  
Katarzyna Śliżewska ◽  
Beata Dolka ◽  
Joanna Nerc ◽  
...  
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Serum Biochemistry ◽  
Control Group ◽  
Normal Ranges ◽  
Rbc Count

In this study, the influence of new multi-strain synbiotics on chicken growth performance, hematology, serum biochemistry and immunity was explored. Each synbiotic preparation (A, B and C) comprised three, four or five strains of Lactobacillus sp., respectively, as well as S. cerevisiae and inulin. All strains used in the synbiotics originated from wild-type strains from animal farms in Poland. Six groups of chickens, ROSS 308 line, were fed with three different synbiotics at a dose of 0.5 g/1 kg of feed. Body weight, as well as the biochemical and hematological parameters of the animals in each study group, were determined on the 7th, 14th, 28th and 42nd day of life. Body weight on day 42 differed between groups and was the highest in control group. This group also had the highestfeed conversion ratio (FCR) value. All measured biochemical parameters were in the normal ranges for poultry; however, we observed a lower alkaline phosphatase (AP) concentration on day 7 in the groups fed with synbiotics, which correlated with a lower level of triglycerides in those groups. The aspartate transaminase (AST) concentration was significantly lower in all groups on day 42 in comparison with the control. On day 7, the control group showed the highest concentration of Ca, K and P. Other parameters did not differ significantly throughout the experiment. All groups showed a similar tendency of increase in the red blood cells (RBC) count according to the age of the birds. Every white blood cells (WBC) population showed differences in the proportions between T and B lymphocytes. The T cell and monocyte counts increased until day 28 in all groups. The results showed that our newly developed synbiotic formulas do not have any unfavorable influence on chicken health and may modulate immune response and biochemical parameters. However, this hypothesis needs to be evaluated in future experiments.

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