scholarly journals Inhibitory strains of Bacillus subtilis for growth and aflatoxin-production of aflatoxigenic fungi.

1988 ◽  
Vol 52 (5) ◽  
pp. 1173-1179 ◽  
Author(s):  
Norio KIMURA ◽  
Susumu HIRANO
Keyword(s):  
Bacillus Subtilis ◽  
Aflatoxin Production ◽  
Aflatoxigenic Fungi

ChemInform Abstract: Aflastatin A, a Novel Inhibitor of Aflatoxin Production by Aflatoxigenic Fungi.

ChemInform ◽  
2010 ◽  
Vol 28 (30) ◽  
pp. no-no
Author(s):  
M. ONO ◽  
S. SAKUDA ◽  
A. SUZUKI ◽  
A. ISOGAI
Keyword(s):  
Aflatoxin Production ◽  
Aflatoxigenic Fungi

scholarly journals Impact of bioactive packaging systems based on EVOH films and essential oils in the control of aflatoxigenic fungi and aflatoxin production in maize

2017 ◽  
Vol 254 ◽  
pp. 36-46 ◽  
Author(s):  
Eva M. Mateo ◽  
José V. Gómez ◽  
Irene Domínguez ◽  
Jose V. Gimeno-Adelantado ◽  
Rufino Mateo-Castro ◽  
...  
Keyword(s):  
Essential Oils ◽  
Aflatoxin Production ◽  
Aflatoxigenic Fungi

Aflatoxin-inhibiting ability of Bacillus subtilis isolated from soil in southern Vietnam

2020 ◽  
Vol 1 (4) ◽  
pp. 31-35
Author(s):  
Le Thi Ngoc An’ ◽  
◽  
T. N. Gryazneva ◽  
Nguyen Ngoc Hai ◽  
◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Animal Feed ◽  
Aflatoxin Production ◽  
Aflatoxin Contamination ◽  
Biologically Active ◽  
Southern Vietnam

Reducing the level of aflatoxin contamination of animal feed using soil-isolated cultures of B. subtilis, showed the prospects of using this type of bacteria for decontamination of feed. A total of 367 B. subtilis cultures were isolated from soil in southern Vietnam and screened for inhibition of aflatoxin production by Aspergillus in vitro. Of these, 34 isolates of biologically active B. subtilis were selected, of which 7 isolates were the most resistant to aflatoxin. These cultures of bacilli after 5 days of cultivation in a mixture with Aspergillus on crushed corn contributed to a 26,76-fold decrease in aflatoxin levels compared to the control. The data obtained indicate that B. subtilis isolates isolated from soil can inhibit aflatoxin in vitro.

Palm Kernel: A Potential Substrate for Rapid Detection of Aflatoxigenic Fungi

2005 ◽  
Vol 11 (1) ◽  
pp. 67-74 ◽  
Author(s):  
O. O. Atanda ◽  
I. Akpan ◽  
E. R. Rati ◽  
M. Ozoje
Keyword(s):  
Uv Light ◽  
Palm Kernel ◽  
Aflatoxin Production ◽  
Water Soluble ◽  
Hot Water ◽  
Optimal Time ◽  
Aflatoxigenic Fungi ◽  
Yellow Pigmentation ◽  
The Tropics ◽  
Layer Chromatography

Palm kernel is a cheap natural resource which is abundantly available in the tropics, parts of Asia and South and Central America. A culture medium was developed by incorporating fresh palm kernel extract for the detection of aflatoxigenic fungi. Aflatoxin positive isolates of Aspergilliexhibited a characteristic blue or blue green fluorescence of agar under long wave UV light against a pink background which was confirmed by thin layer chromatography. As compared to conventional desiccated coconut agar, the fluorescent nature of the medium, the intensity and diffusion of the hot water soluble fluorescent compounds of the fungus was unique on this medium. The optimal pH and temperature conditions of aflatoxin production were 7 and 30 ºC respectively. Additives (synthetic and natural) either had no effect or adversely affected the fluorescence of the medium. Aflatoxin detection was possible within 36h in palm kernel broth compared to 40 h in coconut broth. The optimal time of production of fluorescence was 44 h on palm kernel agar compared to 48 h on the conventional medium. Further tests with isolates from different sources showed that yellow pigmentation, fluorescence and aflatoxins were complementary thus obviating the need for UV light. It is thus possible to presumptively identify aflatoxin positive isolates.

Aflatoxin Production by Aspergillus parasiticus and Its Stability During the Manufacture of Korean Soy Paste (Doenjang) and Soy Sauce (Kanjang) by Traditional Method

1988 ◽  
Vol 51 (12) ◽  
pp. 938-944 ◽  
Author(s):  
KUN-YOUNG PARK ◽  
KYU-BOK LEE ◽  
LLOYD B. BULLERMAN
Keyword(s):  
Bacillus Subtilis ◽  
Mixed Culture ◽  
Traditional Method ◽  
Degradation Rate ◽  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
Toxin Production ◽  
Soy Sauce ◽  
Natural Conditions ◽  
Soybean Cake

Aflatoxin (AF) production and its stability on meju (crushed Korean soybean cake) by a pure culture of Aspergillus parasiticus and a mixed culture of A. parasiticus. A. oryzae and Bacillus subtilis (fermentation 1) during the manufacture by traditional methods of Korean soy paste (doenjang) and soy sauce (kanjang) were studied. There was a difference in the amount of aflatoxin production on different varieties of soybeans, but the pattern of toxin production was similar. During fermentation, more total aflatoxins were produced under the mixed culture condition. Aflatoxin G1 (AFG1) production was highly stimulated though it degraded quickly, whereas aflatoxin B1 (AFB1) synthesis was low. The exposure of the meju to sunlight during fermentation had no effect in reducing aflatoxin synthesis. When the meju fermented under natural conditions (fermentation 2) with a contamination by A. parasiticus, high levels of aflatoxins were still produced. After a month of ripening of the fermented meju with charcoal in brine, more AFG1 than AFB1 was degraded in both the pure and mixed culture samples (degradation %; B1:2–69%, G1:31–84%). When the meju was ripened in water, most of the aflatoxins were degraded (B1:95–99%, G1:100%) in a month, accompanied by a significant increase in pH (p<0.05). A greater amount of aflatoxins (96–100%) was detected in the meju when it was ripened in brine, however, lower amounts (25–85%) of aflatoxins remained in meju ripened in water. During three months of ripening in brine, 83–98% of AFB1 and 98–100% of AFG1 were degraded in fermentation 1, but the degradation rate was slower (B1 :63%, G1:98%) following fermentation 2. The total levels of aflatoxins remaining were significantly (p<0.05) reduced when charcoal was added to the mixture.

scholarly journals Development of an ELISA-Based Method for Testing Aflatoxigenicity and Aflatoxigenic Variability among Aspergillus species in Culture

2019 ◽  
Author(s):  
Lagat Kipkemboi Micah ◽  
Faith Jebet Toroitich ◽  
Meshack Amos Obonyo
Keyword(s):  
Biological Control ◽  
Aflatoxin Production ◽  
Toxin Production ◽  
Field Trials ◽  
Control Programs ◽  
Color Changes ◽  
Visual Color ◽  
Aflatoxigenic Fungi ◽  
Maize Kernels ◽  
First Time

AbstractAflatoxins contaminate foodstuff posing a severe threat to human health because chronic exposure is linked to liver cancer while acute exposure may cause death. Therefore, it is of interest to reduce the contamination of crops by aflatoxins in the field and post-harvest. Among the current technologies being developed is the deployment of non-aflatoxigenic strains of Aspergillus species to competitively exclude aflatoxigenic conspecifics from crops in the field thereby curtailing aflatoxin production by the former. The success in this endeavor makes the non-aflatoxigenic fungi good candidates for biological control programs. However, the current techniques for segregating non-aflatoxigenic from aflatoxigenic fungi suffer two main drawbacks: they are based on morphological and chemical tests with a combination of visual color changes detected in a culture plate which suffer some degree of inaccuracy. Secondly, the existing methods are incapable of accurately quantifying aflatoxin production by fungi in culture. We developed a culture system for inducing aflatoxin production by Aspergillus using maize kernels as growth substrate followed by quantification using ELISA. The method was compared to the Dichlorvos-Ammonia (DV-AM) method for determining aflatoxigenicity. Our findings encapsulate a method more robust than the currently used DV-AM approach because, for the first time, we are able to assess aflatoxigenicity and aflatoxigenic variability among Aspergillus species earlier classified as non-aflatoxigenic by the DV-AM method. Furthermore, the new method presents an opportunity to attribute toxin production by actively growing fungal cultures. We believe this method when further developed presents a chance to study and predict fungal behavior prior to field trials for biological control programs.

scholarly journals Aflastatin A, a Novel Inhibitor of Aflatoxin Production by Aflatoxigenic Fungi.

1997 ◽  
Vol 50 (2) ◽  
pp. 111-118 ◽  
Author(s):  
MAKOTO ONO ◽  
SHOHEI SAKUDA ◽  
AKINORI SUZUKI ◽  
AKIRA ISOGAI
Keyword(s):  
Aflatoxin Production ◽  
Aflatoxigenic Fungi
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