Evaluation of an Effective Sample Prefractionation Method for the Proteome Analysis of Breast Cancer Tissue Using Narrow Range Two-Dimensional Gel Electrophoresis

2008 ◽  
Vol 72 (6) ◽  
pp. 1464-1474 ◽  
Author(s):  
KiBeom LEE
Keyword(s):  
Breast Cancer ◽  
Gel Electrophoresis ◽  
Narrow Range ◽  
Proteome Analysis ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Proteome Analysis of CD4+ T Cells Reveals Differentially Expressed Proteins in Infertile Polycystic Ovary Syndrome Patients

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Nasri ◽  
Maryam Zare ◽  
Mehrnoosh Doroudchi ◽  
Behrouz Gharesi-Fard
Keyword(s):  
Mass Spectrometry ◽  
T Cells ◽  
T Lymphocytes ◽  
Gel Electrophoresis ◽  
Cd4 T Cells ◽  
Polycystic Ovary ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Ovary Syndrome ◽  
Two Dimensional Gel Electrophoresis

Background: Polycystic ovary syndrome (PCOS) is the most frequent endocrine disorder affecting 6–7% of premenopausal women. Recent studies revealed that the immune system especially CD4+ T helper cells are important in the context PCOS. Proteome analysis of CD4+ T lymphocytes can provide valuable information regarding the biology of these cells in the context of PCOS. Objective: To investigate immune dysregulation in CD4+ T lymphocytes at the protein level in the context of PCOS using two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS). Methods: In the present study, we applied two-dimensional gel electrophoresis / mass spectrometry to identify proteins differentially expressed by peripheral blood CD4+ T cells in ten PCOS women compared with ten healthy women. Western blot technique was used to confirm the identified proteins. Results: Despite the overall proteome similarities, there were significant differences in the expression of seven spots between two groups (P <0.05). Three proteins, namely phosphatidylethanolamine-binding protein 1, proteasome activator complex subunit 1 and triosephosphate isomerase 1 were successfully identified by Mass technique and confirmed by western blot. All characterized proteins were over-expressed in CD4+ T cells from patients compared to CD4+ T cells from controls (P <0.05). In-silico analysis suggested that the over-expressed proteins interact with other proteins involved in cellular metabolism especially glycolysis and ferroptosis pathway. Conclusion: These findings suggest that metabolic adjustments in CD4+ T lymphocytes, which is in favor of increased glycolysis and Th2 differentiation are important in the context of PCOS.

scholarly journals Evaluation of narrow range immobiline pH gradient gels in an automated two-dimensional gel electrophoresis machine, Auto2D

2015 ◽  
Vol 59 (1) ◽  
pp. 9-12
Author(s):  
Yuki Tani ◽  
Parlayan Cuneyd ◽  
Yoko Takai ◽  
Akira Kawai ◽  
Yutaka Unuma ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Narrow Range ◽  
Ph Gradient ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Proteome analysis of glandular parotid and submandibular-sublingual saliva in comparison to whole human saliva by two-dimensional gel electrophoresis

PROTEOMICS ◽  
2006 ◽  
Vol 6 (5) ◽  
pp. 1631-1639 ◽  
Author(s):  
Anke Walz ◽  
Kai Stühler ◽  
Andreas Wattenberg ◽  
Eva Hawranke ◽  
Helmut E. Meyer ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Human Saliva ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Proteome analysis of Leishmania (Viannia) braziliensis by two-dimensional gel electrophoresis and mass spectrometry

2007 ◽  
Vol 154 (1) ◽  
pp. 6-21 ◽  
Author(s):  
Patricia Cuervo ◽  
Jose Batista de Jesus ◽  
Magno Junqueira ◽  
Leila Mendonça-Lima ◽  
Luis Javier González ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Protein extraction from mature rice leaves for two-dimensional gel electrophoresis and its application in proteome analysis

PROTEOMICS ◽  
2004 ◽  
Vol 4 (7) ◽  
pp. 1903-1908 ◽  
Author(s):  
Nazrul Islam ◽  
M. Lonsdale ◽  
N. M. Upadhyaya ◽  
T. J. Higgins ◽  
H. Hirano ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Protein Extraction ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Rice Leaves

Attempt at Two-Dimensional Mapping of X-ray Fluorescence from Breast Cancer Tissue

2005 ◽  
Vol 44 (No. 31) ◽  
pp. L998-L1001 ◽  
Author(s):  
Masami Ando ◽  
Katsuhito Yamasaki ◽  
Chiho Ohbayashi ◽  
Hiroyasu Esumi ◽  
Kazuyuki Hyodo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Two Dimensional ◽  
X Ray ◽  
Dimensional Mapping

Proteome analysis ofSchizosaccharomyces pombe by two-dimensional gel electrophoresis and mass spectrometry

PROTEOMICS ◽  
2006 ◽  
Vol 6 (14) ◽  
pp. 4115-4129 ◽  
Author(s):  
Kyung-Hoon Hwang ◽  
Christine Carapito ◽  
Susanne Böhmer ◽  
Emmanuelle Leize ◽  
Alain Van Dorsselaer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis
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