Comparative proteome analysis of three mouse lung adenocarcinoma CMT cell lines with different metastatic potential by two-dimensional gel electrophoresis and mass spectrometry

PROTEOMICS ◽  
2008 ◽  
Vol 8 (23-24) ◽  
pp. 4932-4945 ◽  
Author(s):  
Kelan Zhang ◽  
Krzysztof Wrzesinski ◽  
J. Fey Stephen ◽  
Peter Mose Larsen ◽  
Xumin Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Lung Adenocarcinoma ◽  
Cell Lines ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Metastatic Potential ◽  
Mouse Lung ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Proteome Analysis of CD4+ T Cells Reveals Differentially Expressed Proteins in Infertile Polycystic Ovary Syndrome Patients

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Nasri ◽  
Maryam Zare ◽  
Mehrnoosh Doroudchi ◽  
Behrouz Gharesi-Fard
Keyword(s):  
Mass Spectrometry ◽  
T Cells ◽  
T Lymphocytes ◽  
Gel Electrophoresis ◽  
Cd4 T Cells ◽  
Polycystic Ovary ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Ovary Syndrome ◽  
Two Dimensional Gel Electrophoresis

Background: Polycystic ovary syndrome (PCOS) is the most frequent endocrine disorder affecting 6–7% of premenopausal women. Recent studies revealed that the immune system especially CD4+ T helper cells are important in the context PCOS. Proteome analysis of CD4+ T lymphocytes can provide valuable information regarding the biology of these cells in the context of PCOS. Objective: To investigate immune dysregulation in CD4+ T lymphocytes at the protein level in the context of PCOS using two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS). Methods: In the present study, we applied two-dimensional gel electrophoresis / mass spectrometry to identify proteins differentially expressed by peripheral blood CD4+ T cells in ten PCOS women compared with ten healthy women. Western blot technique was used to confirm the identified proteins. Results: Despite the overall proteome similarities, there were significant differences in the expression of seven spots between two groups (P <0.05). Three proteins, namely phosphatidylethanolamine-binding protein 1, proteasome activator complex subunit 1 and triosephosphate isomerase 1 were successfully identified by Mass technique and confirmed by western blot. All characterized proteins were over-expressed in CD4+ T cells from patients compared to CD4+ T cells from controls (P <0.05). In-silico analysis suggested that the over-expressed proteins interact with other proteins involved in cellular metabolism especially glycolysis and ferroptosis pathway. Conclusion: These findings suggest that metabolic adjustments in CD4+ T lymphocytes, which is in favor of increased glycolysis and Th2 differentiation are important in the context of PCOS.

Proteome analysis of Leishmania (Viannia) braziliensis by two-dimensional gel electrophoresis and mass spectrometry

2007 ◽  
Vol 154 (1) ◽  
pp. 6-21 ◽  
Author(s):  
Patricia Cuervo ◽  
Jose Batista de Jesus ◽  
Magno Junqueira ◽  
Leila Mendonça-Lima ◽  
Luis Javier González ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Proteome analysis ofSchizosaccharomyces pombe by two-dimensional gel electrophoresis and mass spectrometry

PROTEOMICS ◽  
2006 ◽  
Vol 6 (14) ◽  
pp. 4115-4129 ◽  
Author(s):  
Kyung-Hoon Hwang ◽  
Christine Carapito ◽  
Susanne Böhmer ◽  
Emmanuelle Leize ◽  
Alain Van Dorsselaer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

scholarly journals Proteome analysis of Rickettsia conorii by two-dimensional gel electrophoresis coupled with mass spectrometry

2005 ◽  
Vol 245 (2) ◽  
pp. 231-238 ◽  
Author(s):  
Patricia Renesto ◽  
Saïd Azza ◽  
Alain Dolla ◽  
Patrick Fourquet ◽  
Guy Vestris ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Rickettsia Conorii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Glomerulus proteome analysis with two-dimensional gel electrophoresis and mass spectrometry

2007 ◽  
Vol 64 (24) ◽  
pp. 3317-3335 ◽  
Author(s):  
S. Tryggvason ◽  
M. Nukui ◽  
Á. Oddsson ◽  
K. Tryggvason ◽  
H. Jörnvall
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis
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