Proteome analysis ofSchizosaccharomyces pombe by two-dimensional gel electrophoresis and mass spectrometry

PROTEOMICS ◽  
2006 ◽  
Vol 6 (14) ◽  
pp. 4115-4129 ◽  
Author(s):  
Kyung-Hoon Hwang ◽  
Christine Carapito ◽  
Susanne Böhmer ◽  
Emmanuelle Leize ◽  
Alain Van Dorsselaer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Proteome Analysis of CD4+ T Cells Reveals Differentially Expressed Proteins in Infertile Polycystic Ovary Syndrome Patients

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Nasri ◽  
Maryam Zare ◽  
Mehrnoosh Doroudchi ◽  
Behrouz Gharesi-Fard
Keyword(s):  
Mass Spectrometry ◽  
T Cells ◽  
T Lymphocytes ◽  
Gel Electrophoresis ◽  
Cd4 T Cells ◽  
Polycystic Ovary ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Ovary Syndrome ◽  
Two Dimensional Gel Electrophoresis

Background: Polycystic ovary syndrome (PCOS) is the most frequent endocrine disorder affecting 6–7% of premenopausal women. Recent studies revealed that the immune system especially CD4+ T helper cells are important in the context PCOS. Proteome analysis of CD4+ T lymphocytes can provide valuable information regarding the biology of these cells in the context of PCOS. Objective: To investigate immune dysregulation in CD4+ T lymphocytes at the protein level in the context of PCOS using two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS). Methods: In the present study, we applied two-dimensional gel electrophoresis / mass spectrometry to identify proteins differentially expressed by peripheral blood CD4+ T cells in ten PCOS women compared with ten healthy women. Western blot technique was used to confirm the identified proteins. Results: Despite the overall proteome similarities, there were significant differences in the expression of seven spots between two groups (P <0.05). Three proteins, namely phosphatidylethanolamine-binding protein 1, proteasome activator complex subunit 1 and triosephosphate isomerase 1 were successfully identified by Mass technique and confirmed by western blot. All characterized proteins were over-expressed in CD4+ T cells from patients compared to CD4+ T cells from controls (P <0.05). In-silico analysis suggested that the over-expressed proteins interact with other proteins involved in cellular metabolism especially glycolysis and ferroptosis pathway. Conclusion: These findings suggest that metabolic adjustments in CD4+ T lymphocytes, which is in favor of increased glycolysis and Th2 differentiation are important in the context of PCOS.

Proteome analysis of Leishmania (Viannia) braziliensis by two-dimensional gel electrophoresis and mass spectrometry

2007 ◽  
Vol 154 (1) ◽  
pp. 6-21 ◽  
Author(s):  
Patricia Cuervo ◽  
Jose Batista de Jesus ◽  
Magno Junqueira ◽  
Leila Mendonça-Lima ◽  
Luis Javier González ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

scholarly journals Proteome analysis of Rickettsia conorii by two-dimensional gel electrophoresis coupled with mass spectrometry

2005 ◽  
Vol 245 (2) ◽  
pp. 231-238 ◽  
Author(s):  
Patricia Renesto ◽  
Saïd Azza ◽  
Alain Dolla ◽  
Patrick Fourquet ◽  
Guy Vestris ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Rickettsia Conorii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Glomerulus proteome analysis with two-dimensional gel electrophoresis and mass spectrometry

2007 ◽  
Vol 64 (24) ◽  
pp. 3317-3335 ◽  
Author(s):  
S. Tryggvason ◽  
M. Nukui ◽  
Á. Oddsson ◽  
K. Tryggvason ◽  
H. Jörnvall
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

scholarly journals Proteomic analysis of the pulvinus, a heliotropic tissue, in Glycine max

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Hakme Lee ◽  
Wesley M. Garrett ◽  
Joseph Sullivan ◽  
Irwin Forseth ◽  
Savithiry S. Natarajan
Keyword(s):  
Mass Spectrometry ◽  
Gene Ontology ◽  
Gel Electrophoresis ◽  
Proton Transport ◽  
Enrichment Analysis ◽  
The Sun ◽  
Leaf Movement ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Leguminous Plants

Certain plant species respond to light, dark, and other environmental factors by leaf movement. Leguminous plants both track and avoid the sun through turgor changes of the pulvinus tissue at the base of leaves. Mechanisms leading to pulvinar turgor flux, particularly knowledge of the proteins involved, are not well-known. In this study we used two-dimensional gel electrophoresis and liquid chromatography-tandom mass spectrometry to separate and identify the proteins located in the soybean pulvinus. A total of 183 spots were separated and 195 proteins from 165 spots were identified and functionally analyzed using single enrichment analysis for gene ontology terms. The most significant terms were related to proton transport. Comparison with guard cell proteomes revealed similar significant processes but a greater number of pulvinus proteins are required for comparable analysis. To our knowledge, this is a novel report on the analysis of proteins found in soybean pulvinus. These findings provide a better understanding of the proteins required for turgor change in the pulvinus.

Sign in / Sign up

Export Citation Format

Share Document