scholarly journals Characterization of Clofibrate-induced Retrograde Golgi Membrane Movement to the Endoplasmic Reticulum: Clofibrate Distinguishes the Golgi from the Trans Golgi Network

2001 ◽  
Vol 65 (8) ◽  
pp. 1812-1823 ◽  
Author(s):  
Machiko NAKAMURA ◽  
Natsuko KUROIWA ◽  
Yoshiki KONO ◽  
Akira TAKATSUKI
Keyword(s):  
Endoplasmic Reticulum ◽  
Golgi Membrane ◽  
Trans Golgi Network ◽  
Golgi Network

scholarly journals Detection of angiotensin II‐induced Ras activation in the trans‐Golgi network and the endoplasmic reticulum using BRET‐based biosensors

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Laszló Hunyady ◽  
Eszter Soltész‐Katona ◽  
László Erdélyi ◽  
Péter Várnai ◽  
András Balla
Keyword(s):  
Endoplasmic Reticulum ◽  
Angiotensin Ii ◽  
Trans Golgi Network ◽  
Ras Activation ◽  
Golgi Network

scholarly journals A new role for RINT-1 in SNARE complex assembly at the trans-Golgi network in coordination with the COG complex

2013 ◽  
Vol 24 (18) ◽  
pp. 2907-2917 ◽  
Author(s):  
Kohei Arasaki ◽  
Daichi Takagi ◽  
Akiko Furuno ◽  
Miwa Sohda ◽  
Yoshio Misumi ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Membrane Trafficking ◽  
Retrograde Transport ◽  
Snare Complex ◽  
Initial Contact ◽  
Complex Assembly ◽  
Trans Golgi Network ◽  
Cog Complex ◽  
Protein Receptor ◽  
Golgi Network

Docking and fusion of transport vesicles/carriers with the target membrane involve a tethering factor–mediated initial contact followed by soluble N-ethylmaleimide–sensitive factor attachment protein receptor (SNARE)–catalyzed membrane fusion. The multisubunit tethering CATCHR family complexes (Dsl1, COG, exocyst, and GARP complexes) share very low sequence homology among subunits despite likely evolving from a common ancestor and participate in fundamentally different membrane trafficking pathways. Yeast Tip20, as a subunit of the Dsl1 complex, has been implicated in retrograde transport from the Golgi apparatus to the endoplasmic reticulum. Our previous study showed that RINT-1, the mammalian counterpart of yeast Tip20, mediates the association of ZW10 (mammalian Dsl1) with endoplasmic reticulum–localized SNARE proteins. In the present study, we show that RINT-1 is also required for endosome-to–trans-Golgi network trafficking. RINT-1 uncomplexed with ZW10 interacts with the COG complex, another member of the CATCHR family complex, and regulates SNARE complex assembly at the trans-Golgi network. This additional role for RINT-1 may in part reflect adaptation to the demand for more diverse transport routes from endosomes to the trans-Golgi network in mammals compared with those in a unicellular organism, yeast. The present findings highlight a new role of RINT-1 in coordination with the COG complex.

A genetic and molecular characterization of the garnet gene of Drosophila melanogaster

Genome ◽  
1999 ◽  
Vol 42 (6) ◽  
pp. 1183-1193 ◽  
Author(s):  
Vett K Lloyd ◽  
D A Sinclair ◽  
R Wennberg ◽  
T S Warner ◽  
B M Honda ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Pigment Granule ◽  
Initial Step ◽  
Genetic Dissection ◽  
Trans Golgi Network ◽  
Intracellular Vesicle ◽  
Intracellular Sorting ◽  
Golgi Network ◽  
Delta Subunit

The garnet gene was one of the first genes to be identified in Drosophila melanogaster. Mutations in the garnet gene affect both of the biochemically distinct types of pigments in the eye and disrupt pigmentation of other organs. As an initial step in the analysis of this gene, we have analyzed the pigmentation defects in several of the garnet alleles. We have also cloned the gene and examined its expression in various tissues and at different stages of development. The garnet gene is expressed throughout development and in all tissues examined. Structurally related sequences can be detected in a variety of other eukaryotes. The predicted protein sequence of the garnet product resembles clathrin and nonclathrin adaptin proteins and is highly similar to the delta subunit of the newly isolated mammalian AP-3 adaptin complex, which is associated with the trans-Golgi network and endosomes. This suggests that garnet encodes a protein that acts in the intracellular sorting and trafficking of vesicles from the trans-Golgi network to endosomes, and related specialized organelles such as the pigment granule. This finding provides an explanation for the phenotype of garnet mutations and predicts that other Drosophila eye-colour genes will be a rich resource for the genetic dissection of intracellular vesicle transport.Key words: garnet, Drosophila melanogaster, AP-3, eye pigments.

scholarly journals Proteomic Characterization of Isolated Arabidopsis Clathrin-Coated Vesicles Reveals Evolutionarily Conserved and Plant Specific Components

2021 ◽  
Author(s):  
Dana A. Dahhan ◽  
Gregory D. Reynolds ◽  
Jessica J. Cárdenas ◽  
Dominique Eeckhout ◽  
Alexander Johnson ◽  
...  
Keyword(s):  
Secretory Pathway ◽  
Adaptor Protein ◽  
Coated Vesicles ◽  
Mass Spectrometry Analysis ◽  
Chemical Labeling ◽  
Spectrometry Analysis ◽  
Trans Golgi Network ◽  
Evolutionarily Conserved ◽  
Golgi Network

In eukaryotes, clathrin-coated vesicles (CCVs) facilitate the internalization of material from the cell surface as well as the movement of cargo in post-Golgi trafficking pathways. This diversity of functions is partially provided by multiple monomeric and multimeric clathrin adaptor complexes that provide compartment and cargo selectivity. The adaptor-protein AP-1 complex operates as part of the secretory pathway at the trans-Golgi network, while the AP-2 complex and the TPLATE complex (TPC) jointly operate at the plasma membrane to execute clathrin-mediated endocytosis. Key to our further understanding of clathrin-mediated trafficking in plants will be the comprehensive identification and characterization of the network of evolutionarily conserved and plant-specific core and accessory machinery involved in the formation and targeting of CCVs. To facilitate these studies, we have analyzed the proteome of enriched trans-Golgi network/early endosome-derived and endocytic CCVs isolated from dividing and expanding suspension-cultured Arabidopsis cells. Tandem mass spectrometry analysis results were validated by differential chemical labeling experiments to identify proteins co-enriching with CCVs. Proteins enriched in CCVs included previously characterized CCV components and cargos such as the vacuolar sorting receptors in addition to conserved and plant-specific components whose function in clathrin-mediated trafficking has not been previously defined. Notably, in addition to AP-1 and AP-2, all subunits of the AP-4 complex, but not AP-3 or AP-5, were found to be in high abundance in the CCV proteome. The association of AP-4 with suspension-cultured Arabidopsis CCVs is further supported via additional biochemical data.

scholarly journals Autoantigen Golgin-97, an Effector of Arl1 GTPase, Participates in Traffic from the Endosome to the Trans-Golgi Network

2004 ◽  
Vol 15 (10) ◽  
pp. 4426-4443 ◽  
Author(s):  
Lei Lu ◽  
Guihua Tai ◽  
Wanjin Hong
Keyword(s):  
Functional Study ◽  
Toxin B ◽  
Membrane Recruitment ◽  
Trans Golgi Network ◽  
Transport Assay ◽  
Retrograde Traffic ◽  
Golgi Network

The precise cellular function of Arl1 and its effectors, the GRIP domain Golgins, is not resolved, despite our recent understanding that Arl1 regulates the membrane recruitment of these Golgins. In this report, we describe our functional study of Golgin-97. Using a Shiga toxin B fragment (STxB)-based in vitro transport assay, we demonstrated that Golgin-97 plays a role in transport from the endosome to the trans-Golgi network (TGN). The recombinant GRIP domain of Golgin-97 as well as antibodies against Golgin-97 inhibited the transport of STxB in vitro. Membrane-associated Golgin-97, but not its cytosolic pool, was required in the in vitro transport assay. The kinetic characterization of inhibition by anti-Golgin-97 antibody in comparison with anti-Syntaxin 16 antibody established that Golgin-97 acts before Syntaxin 16 in endosome-to-TGN transport. Knock down of Golgin-97 or Arl1 by their respective small interference RNAs (siRNAs) also significantly inhibited the transport of STxB to the Golgi in vivo. In siRNA-treated cells with reduced levels of Arl1, internalized STxB was instead distributed peripherally. Microinjection of Golgin-97 antibody led to the fragmentation of Golgi apparatus and the arrested transport to the Golgi of internalized Cholera toxin B fragment. We suggest that Golgin-97 may function as a tethering molecule in endosome-to-TGN retrograde traffic.

scholarly journals Endoplasmic reticulum and trans-Golgi network generate distinct populations of Alzheimer beta -amyloid peptides

1999 ◽  
Vol 96 (2) ◽  
pp. 742-747 ◽  
Author(s):  
J. P. Greenfield ◽  
J. Tsai ◽  
G. K. Gouras ◽  
B. Hai ◽  
G. Thinakaran ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Beta Amyloid ◽  
Amyloid Peptides ◽  
Trans Golgi Network ◽  
Golgi Network
Sign in / Sign up

Export Citation Format

Share Document