scholarly journals Functional characterization of protein-sorting machineries at the trans-Golgi network in Drosophila melanogaster

2010 ◽  
Vol 123 (3) ◽  
pp. 460-471 ◽  
Author(s):  
S. Kametaka ◽  
N. Sawada ◽  
J. S. Bonifacino ◽  
S. Waguri
Keyword(s):  
Drosophila Melanogaster ◽  
Protein Sorting ◽  
Functional Characterization ◽  
Trans Golgi Network ◽  
Golgi Network

A genetic and molecular characterization of the garnet gene of Drosophila melanogaster

Genome ◽  
1999 ◽  
Vol 42 (6) ◽  
pp. 1183-1193 ◽  
Author(s):  
Vett K Lloyd ◽  
D A Sinclair ◽  
R Wennberg ◽  
T S Warner ◽  
B M Honda ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Pigment Granule ◽  
Initial Step ◽  
Genetic Dissection ◽  
Trans Golgi Network ◽  
Intracellular Vesicle ◽  
Intracellular Sorting ◽  
Golgi Network ◽  
Delta Subunit

The garnet gene was one of the first genes to be identified in Drosophila melanogaster. Mutations in the garnet gene affect both of the biochemically distinct types of pigments in the eye and disrupt pigmentation of other organs. As an initial step in the analysis of this gene, we have analyzed the pigmentation defects in several of the garnet alleles. We have also cloned the gene and examined its expression in various tissues and at different stages of development. The garnet gene is expressed throughout development and in all tissues examined. Structurally related sequences can be detected in a variety of other eukaryotes. The predicted protein sequence of the garnet product resembles clathrin and nonclathrin adaptin proteins and is highly similar to the delta subunit of the newly isolated mammalian AP-3 adaptin complex, which is associated with the trans-Golgi network and endosomes. This suggests that garnet encodes a protein that acts in the intracellular sorting and trafficking of vesicles from the trans-Golgi network to endosomes, and related specialized organelles such as the pigment granule. This finding provides an explanation for the phenotype of garnet mutations and predicts that other Drosophila eye-colour genes will be a rich resource for the genetic dissection of intracellular vesicle transport.Key words: garnet, Drosophila melanogaster, AP-3, eye pigments.

Functional characterization of HLA-DRA11 0101/ DRB1* 0401 molecules expressed in Drosophila melanogaster cells

2008 ◽  
Vol 51 (2) ◽  
pp. 119-128 ◽  
Author(s):  
Bjarke Endel Hansen ◽  
Ellen Christina Andersson ◽  
Lars Siim Madsen ◽  
Jan Engberg ◽  
Leif Søndergaard ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Functional Characterization

scholarly journals Functional characterization of Drosophila melanogaster PERK eukaryotic initiation factor 2alpha (eIF2alpha) kinase

2003 ◽  
Vol 270 (2) ◽  
pp. 293-306 ◽  
Author(s):  
Natalia Pomar ◽  
Juan J. Berlanga ◽  
Sonsoles Campuzano ◽  
Greco Hernandez ◽  
Monica Elias ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Functional Characterization ◽  
Initiation Factor ◽  
Eukaryotic Initiation Factor ◽  
Eif2alpha Kinase

scholarly journals Proteomic Characterization of Isolated Arabidopsis Clathrin-Coated Vesicles Reveals Evolutionarily Conserved and Plant Specific Components

2021 ◽  
Author(s):  
Dana A. Dahhan ◽  
Gregory D. Reynolds ◽  
Jessica J. Cárdenas ◽  
Dominique Eeckhout ◽  
Alexander Johnson ◽  
...  
Keyword(s):  
Secretory Pathway ◽  
Adaptor Protein ◽  
Coated Vesicles ◽  
Mass Spectrometry Analysis ◽  
Chemical Labeling ◽  
Spectrometry Analysis ◽  
Trans Golgi Network ◽  
Evolutionarily Conserved ◽  
Golgi Network

In eukaryotes, clathrin-coated vesicles (CCVs) facilitate the internalization of material from the cell surface as well as the movement of cargo in post-Golgi trafficking pathways. This diversity of functions is partially provided by multiple monomeric and multimeric clathrin adaptor complexes that provide compartment and cargo selectivity. The adaptor-protein AP-1 complex operates as part of the secretory pathway at the trans-Golgi network, while the AP-2 complex and the TPLATE complex (TPC) jointly operate at the plasma membrane to execute clathrin-mediated endocytosis. Key to our further understanding of clathrin-mediated trafficking in plants will be the comprehensive identification and characterization of the network of evolutionarily conserved and plant-specific core and accessory machinery involved in the formation and targeting of CCVs. To facilitate these studies, we have analyzed the proteome of enriched trans-Golgi network/early endosome-derived and endocytic CCVs isolated from dividing and expanding suspension-cultured Arabidopsis cells. Tandem mass spectrometry analysis results were validated by differential chemical labeling experiments to identify proteins co-enriching with CCVs. Proteins enriched in CCVs included previously characterized CCV components and cargos such as the vacuolar sorting receptors in addition to conserved and plant-specific components whose function in clathrin-mediated trafficking has not been previously defined. Notably, in addition to AP-1 and AP-2, all subunits of the AP-4 complex, but not AP-3 or AP-5, were found to be in high abundance in the CCV proteome. The association of AP-4 with suspension-cultured Arabidopsis CCVs is further supported via additional biochemical data.

scholarly journals Systematic functional characterization of putative zinc transport genes and identification of zinc toxicosis phenotypes in Drosophila melanogaster

2012 ◽  
Vol 215 (18) ◽  
pp. 3254-3265 ◽  
Author(s):  
J. C. Lye ◽  
C. D. Richards ◽  
K. Dechen ◽  
D. Paterson ◽  
M. D. de Jonge ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Functional Characterization ◽  
Zinc Transport
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