Production of Recombinant Mite Allergen Der fI in Insect Cells and Characterization of Products———Removal of Pro-sequence Is Essential to IgE-Binding Activity——

1996 ◽  
Vol 60 (4) ◽  
pp. 621-625 ◽  
Author(s):  
Hiroshi Shoji ◽  
Masayoshi Hanawa ◽  
Ichiro Shibuya ◽  
Mitsuo Hirai ◽  
Takaomi Yasuhara ◽  
...  
Keyword(s):  
Insect Cells ◽  
Binding Activity ◽  
Mite Allergen ◽  
Ige Binding

Isolation and Characterization of a Relevant Aspergillus fumigatus Antigen with IgG- and IgE-Binding Activity

1988 ◽  
Vol 86 (2) ◽  
pp. 176-182 ◽  
Author(s):  
Viswanath P. Kurup ◽  
Muthiah Ramasamy ◽  
Paul A. Greenberger ◽  
Jordan N. Fink
Keyword(s):  
Aspergillus Fumigatus ◽  
Binding Activity ◽  
Isolation And Characterization ◽  
Ige Binding

Expression and Characterization of Human Dopamine D2Receptor in Baculovirus-Infected Insect Cells

1994 ◽  
Vol 14 (2) ◽  
pp. 99-117 ◽  
Author(s):  
J. A. Javitch ◽  
J. Kaback ◽  
X. Li ◽  
A. Karlin
Keyword(s):  
Insect Cells ◽  
Infected Insect

scholarly journals Androgen-sensitive spermine-binding protein of rat ventral prostate. Purification of the protein and characterization of the hormonal effect

1979 ◽  
Vol 184 (2) ◽  
pp. 431-440 ◽  
Author(s):  
G Mezzetti ◽  
R Loor ◽  
S Liao
Keyword(s):  
Binding Protein ◽  
Sodium Dodecyl ◽  
Deae Cellulose ◽  
Binding Activity ◽  
Ventral Prostate ◽  
Hormonal Effect ◽  
Rat Ventral Prostate ◽  
Acidic Fraction ◽  
Androgen Effect

The rat ventral prostate contains a cytosol protein that can non-covalently bind spermine much more tightly than spermidine or other natural diamines. The protein has been purified to homogeneity, as judged by electrophoresis in urea- and sodium dodecyl sulphate-containing polyacrylamide gels. The protein, with or without spermine bound to it, sediments at 3 S in a sucrose gradient with or without 0.4 M-KCl. The molecular weight of the protein is about 30 000. Each molecule of the binding protein can bind one molecule of spermine. In the prostate of rats injected with cycloheximide, the protein appears to have a half-life of about 3.5 h. The spermine-binding activity of an acidic fraction obtained by DEAE-cellulose chromatography of the prostate cytosol proteins is reduced by about 40–60% within 20–40 h after castration. This effect is reversed very rapidly within 15–30 min by intraperitoneal injection of 5 alpha-dihydrotestosterone. The hormonal effect is androgen-specific and is not mimicked by dexamethasone or oestradiol-17 beta. The androgen effect was reduced significantly when rats were injected with cycloheximide or actinomycin D, suggesting that the acidic protein may be one of the earliest proteins induced by androgen in the rat ventral prostate.

Characterization of the DNA Binding Activity of the ZFY Zinc Finger Domain

Biochemistry ◽  
2010 ◽  
Vol 49 (4) ◽  
pp. 679-686 ◽  
Author(s):  
Jennifer Grants ◽  
Erin Flanagan ◽  
Andrea Yee ◽  
Paul J. Romaniuk
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Binding Activity ◽  
Zinc Finger Domain ◽  
Dna Binding Activity

scholarly journals Biochemical characterization of L1 repressor mutants with altered operator DNA binding activity

Bacteriophage ◽  
2012 ◽  
Vol 2 (2) ◽  
pp. 79-88 ◽  
Author(s):  
Amitava Bandhu ◽  
Tridib Ganguly ◽  
Biswanath Jana ◽  
Amritangshu Chakravarty ◽  
Anindya Biswas ◽  
...  
Keyword(s):  
Dna Binding ◽  
Biochemical Characterization ◽  
Binding Activity ◽  
Dna Binding Activity ◽  
Operator Dna
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