Isolation and Characterization of a Relevant Aspergillus fumigatus Antigen with IgG- and IgE-Binding Activity

1988 ◽  
Vol 86 (2) ◽  
pp. 176-182 ◽  
Author(s):  
Viswanath P. Kurup ◽  
Muthiah Ramasamy ◽  
Paul A. Greenberger ◽  
Jordan N. Fink
Keyword(s):  
Aspergillus Fumigatus ◽  
Binding Activity ◽  
Isolation And Characterization ◽  
Ige Binding

Production of Recombinant Mite Allergen Der fI in Insect Cells and Characterization of Products———Removal of Pro-sequence Is Essential to IgE-Binding Activity——

1996 ◽  
Vol 60 (4) ◽  
pp. 621-625 ◽  
Author(s):  
Hiroshi Shoji ◽  
Masayoshi Hanawa ◽  
Ichiro Shibuya ◽  
Mitsuo Hirai ◽  
Takaomi Yasuhara ◽  
...  
Keyword(s):  
Insect Cells ◽  
Binding Activity ◽  
Mite Allergen ◽  
Ige Binding

scholarly journals Isolation and Characterization of a 66-kDa Antigen from Culture Filtrate of Aspergillus fumigatus NCPF2109.

1995 ◽  
Vol 36 (1) ◽  
pp. 25-32
Author(s):  
Jotika Boon-Long ◽  
Takeshi Mikami ◽  
Tomonori Murakami ◽  
Tatsuji Matsumoto ◽  
Minehiro Tojo ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Culture Filtrate ◽  
Isolation And Characterization

Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding

FEBS Journal ◽  
2005 ◽  
Vol 272 (10) ◽  
pp. 2536-2544 ◽  
Author(s):  
Lucía Garciá-Ortega ◽  
Javier Lacadena ◽  
Mayte Villalba ◽  
Rosalía Rodríguez ◽  
Jesús F. Crespo ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Deletion Variant ◽  
Ige Binding

Isolation and characterization of distinct bioactive forms of LH from male buffalo pituitaries: differences localized to their α subunits

1994 ◽  
Vol 143 (2) ◽  
pp. 313-323 ◽  
Author(s):  
M R Sairam ◽  
A A H Zaky ◽  
A A Hassan
Keyword(s):  
Receptor Binding ◽  
Binding Activity ◽  
Α Subunit ◽  
Basic Fraction ◽  
Isolation And Characterization ◽  
Receptor Binding Activity ◽  
Acidic Fraction ◽  
Α Subunits

Abstract The isolation of highly purified forms of pituitary LH from Egyptian male (Nile) buffaloes is described. The total LH content (receptor binding activity) which was approximately 30 to 50 fold higher than FSH in the pituitary could be divided into three pools based upon fractionation patterns on a cation exchanger. The acidic fraction which also contained FSH was not purified to homogeneity. A basic fraction (bu-LH-2; 300 mg/kg anterior pituitary) and a very basic fraction (bu-LH-3; 80 mg/kg) were both highly purified and free of FSH activity as tested by specific FSH receptor and immunoassays. The basic buffalo LH fraction, bu-LH-2, was as active as highly purified ovine LH (oLH). The most basic form of buffalo LH, bu-LH-3, was, however, about twice as active as highly purified oLH in the in vitro bioassay using mouse Leydig tumour (MA-10) cells. In a receptor binding assay employing 125I-labelled buffalo LH (bu-LH-3) and porcine testicular membranes, the affinity of bu-LH-3 was about five times higher than purified oLH. The Mr of both forms of purified buffalo LH and subunits was similar to that of oLH. Amino acid composition of buffalo LH was also very similar to oLH except for small differences. Fractionation by fast protein liquid chromatography on Mono-Q columns revealed further evidence of microheterogeneity in each of the pools of buffalo LH with bu-LH-3 exhibiting a predominant single component. By reverse-phase high-pressure liquid chromotography analysis we have localized differences in the two purified isoforms of male buffalo LH to the α subunit. It is suggested that differences in biological potencies could be due to variations in terminal glycosylation and/or differences in branching of this subunit which is known to be important for signal transduction. Journal of Endocrinology (1994) 143, 313–323

Isolation and Characterization of a cDNA Clone Encoding One IgE-Binding Fragment of Penicillium brevicompactum

2005 ◽  
Vol 138 (1) ◽  
pp. 12-20 ◽  
Author(s):  
M.Serdal Sevinc ◽  
Veena Kumar ◽  
Makonnen Abebe ◽  
William L. Casley ◽  
Hari M. Vijay
Keyword(s):  
Cdna Clone ◽  
Isolation And Characterization ◽  
Ige Binding ◽  
Penicillium Brevicompactum

scholarly journals Isolation and Characterization of ElastolyticProteinase from Aspergillus fumigatus.

1995 ◽  
Vol 36 (3) ◽  
pp. 235-243 ◽  
Author(s):  
Yoichi Hasegawa ◽  
Toshiaki Nikai ◽  
Reiko Yamashita ◽  
Yukie Yoshikawa ◽  
Hisayoshi Sugihara ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Isolation And Characterization
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