Characterization of the DNA Binding Activity of the ZFY Zinc Finger Domain

Biochemistry ◽  
2010 ◽  
Vol 49 (4) ◽  
pp. 679-686 ◽  
Author(s):  
Jennifer Grants ◽  
Erin Flanagan ◽  
Andrea Yee ◽  
Paul J. Romaniuk
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Binding Activity ◽  
Zinc Finger Domain ◽  
Dna Binding Activity

scholarly journals Interplay between HIV-1 Vpr and Sp1 Modulates p21WAF1Gene Expression in Human Astrocytes

2004 ◽  
Vol 279 (44) ◽  
pp. 46046-46056 ◽  
Author(s):  
Shohreh Amini ◽  
Marcus Saunders ◽  
Kimberly Kelley ◽  
Kamel Khalili ◽  
Bassel E. Sawaya
Keyword(s):  
Cell Cycle ◽  
Human Immunodeficiency Virus ◽  
Dna Binding ◽  
Zinc Finger ◽  
Human Immunodeficiency Virus Type ◽  
Binding Activity ◽  
Zinc Finger Domain ◽  
Dna Binding Activity ◽  
Immunodeficiency Virus

The Vpr (viral protein R) of human immunodeficiency virus, type 1, which is expressed during the late stage of the viral infection, has received special attention because of its ability to control transcription of the human immunodeficiency virus, type 1, long terminal repeat and to influence cell cycle progression. Here we demonstrate that Vpr has the ability to regulate transcription of the cyclin-dependent kinase inhibitor, p21WAF1(p21), one of the key regulators of the cell cycle, in human astrocytic cells. The results from transcription assays demonstrated that Vpr augments promoter activity of p21 through the GC-rich region located between nucleotides -84 and -74 with respect to the +1 transcription start site. Activation of p21 by Vpr required cooperativity of Sp1, which binds to the DNA sequence spanning -84 to -74. Results from bandshift assay revealed an increased level of Sp1 DNA binding activity in the presence of Vpr. Furthermore, Vpr was able to associate with Sp1 via the zinc finger domain located in the C-terminal region of Sp1. Functional studies revealed that the cooperativity between Vpr and Sp1 requires the zinc finger domain at the C terminus and the glutamine-rich domain at the N terminus of Sp1. Expression of p53 further enhanced the level of Vpr-Sp1-mediated transcription activation of p21 through the sequence spanning -84 to -74 and increased the DNA binding activity of Sp1 in the presence of Vpr. Results from glutathioneS-transferase pull-down assay showed the association of Vpr with p53 in extracts containing Sp1. Altogether, the outcome of our functional and binding studies suggested that the physical interaction of Vpr with Sp1 and p53 could modulate transcriptional activity of p21.

scholarly journals The Human Monocytic Leukemia Zinc Finger Histone Acetyltransferase Domain Contains DNA-binding Activity Implicated in Chromatin Targeting

2007 ◽  
Vol 282 (50) ◽  
pp. 36603-36613 ◽  
Author(s):  
Marc A. Holbert ◽  
Timothy Sikorski ◽  
Juliana Carten ◽  
Danielle Snowflack ◽  
Santosh Hodawadekar ◽  
...  
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Histone Acetyltransferase ◽  
Binding Activity ◽  
Monocytic Leukemia ◽  
Dna Binding Activity ◽  
Human Monocytic Leukemia ◽  
Acetyltransferase Domain

scholarly journals Biochemical characterization of L1 repressor mutants with altered operator DNA binding activity

Bacteriophage ◽  
2012 ◽  
Vol 2 (2) ◽  
pp. 79-88 ◽  
Author(s):  
Amitava Bandhu ◽  
Tridib Ganguly ◽  
Biswanath Jana ◽  
Amritangshu Chakravarty ◽  
Anindya Biswas ◽  
...  
Keyword(s):  
Dna Binding ◽  
Biochemical Characterization ◽  
Binding Activity ◽  
Dna Binding Activity ◽  
Operator Dna

scholarly journals Characterization of the Novel DNA Binding Activity of the BRG1 At-Hook-Bromodomain and Effect of Cancer Mutations

2018 ◽  
Vol 114 (3) ◽  
pp. 569a
Author(s):  
Julio C. Sanchez ◽  
Liyang Zhang ◽  
Amber Liu ◽  
Miles A. Pufall ◽  
Catherine A. Musselman
Keyword(s):  
Dna Binding ◽  
Binding Activity ◽  
The Novel ◽  
Dna Binding Activity ◽  
Cancer Mutations

scholarly journals Characterization of the Novel DNA Binding Activity of the BRG1 At-Hook-Bromodomain

2019 ◽  
Vol 116 (3) ◽  
pp. 332a
Author(s):  
Julio C. Sanchez ◽  
Liyang Zhang Zhang ◽  
Miles Pufall ◽  
Catherine Musselman
Keyword(s):  
Dna Binding ◽  
Binding Activity ◽  
The Novel ◽  
Dna Binding Activity

scholarly journals Identification, nuclear localization, and DNA-binding activity of the zinc finger protein encoded by the Evi-1 myeloid transforming gene.

1990 ◽  
Vol 10 (3) ◽  
pp. 1259-1264 ◽  
Author(s):  
T Matsugi ◽  
K Morishita ◽  
J N Ihle
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Nuclear Protein ◽  
Zinc Finger Protein ◽  
Binding Activity ◽  
Dna Binding Activity ◽  
Double Stranded Dna ◽  
Finger Protein ◽  
Myeloid Leukemias ◽  
Transforming Gene

Activation of the Evi-1 zinc finger gene is a common event associated with transformation of murine myeloid leukemias. To characterize the gene product, we developed antisera against various protein domains. These antisera primarily detected a 145-kilodalton nuclear protein that bound double-stranded DNA. Binding was inhibited by chelating agents and partially restored by zinc ions.

Characterization of the DNA-Binding Activity of HIV-1 Integrase Using a Filter Binding Assay

1995 ◽  
Vol 217 (3) ◽  
pp. 802-810 ◽  
Author(s):  
I.R. Haugan ◽  
B.M. Nilsen ◽  
S. Worland ◽  
L. Olsen ◽  
D.E. Helland
Keyword(s):  
Dna Binding ◽  
Binding Assay ◽  
Binding Activity ◽  
Dna Binding Activity ◽  
Hiv 1
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