A Cysteine Endopeptidase (“Dionain”) Is Involved in the Digestive Fluid ofDionaea muscipula(Venus’s Fly-trap)

2011 ◽  
Vol 75 (2) ◽  
pp. 346-348 ◽  
Author(s):  
Kenji TAKAHASHI ◽  
Takehiro SUZUKI ◽  
Wataru NISHII ◽  
Keiko KUBOTA ◽  
Chiaki SHIBATA ◽  
...  
Keyword(s):  
Cysteine Endopeptidase ◽  
Digestive Fluid

scholarly journals Abundance of Cysteine Endopeptidase Dionain in Digestive Fluid of Venus Flytrap (Dionaea muscipula Ellis) Is Regulated by Different Stimuli from Prey through Jasmonates

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e104424 ◽  
Author(s):  
Michaela Libiaková ◽  
Kristýna Floková ◽  
Ondřej Novák ◽  
L'udmila Slováková ◽  
Andrej Pavlovič
Keyword(s):  
Cysteine Endopeptidase ◽  
Venus Flytrap ◽  
Dionaea Muscipula ◽  
Digestive Fluid

Using Digestive Fluid Biomarkers to Predict Acute Gastrointestinal Injury in Critically Ill Patients: A Pilot Study

2018 ◽  
Vol 27 (6) ◽  
pp. 504-507
Author(s):  
Cheng Minhua ◽  
Gao Tao ◽  
Xi Fengchan ◽  
Yu Wenkui
Keyword(s):  
Intensive Care ◽  
Immunoglobulin A ◽  
Interleukin 10 ◽  
Secretory Immunoglobulin A ◽  
Gastrointestinal Injury ◽  
Gastrointestinal Failure ◽  
Acute Gastrointestinal Injury ◽  
Secretory Immunoglobulin ◽  
Grade Iii ◽  
Digestive Fluid

Background Acute gastrointestinal injury is associated with significantly increased mortality in critically ill patients. However, markers for measuring acute gastrointestinal injury are neither sensitive nor specific. Objective To determine whether enzymes in digestive fluid are predictive of the severity of acute gastrointestinal injury. Methods A prospective observational study was conducted between June 2015 and December 2015 in a surgical intensive care unit. Enrolled patients were classified by acute gastrointestinal injury grade according to the 2012 European Society of Intensive Care Medicine system. Digestive fluid was collected through nasointestinal tubes and analyzed 24 hours after the diagnosis of acute gastrointestinal injury. Intestinal markers of injury (pH, interleukin 6, interleukin 10, tumor necrosis factor α, and secretory immunoglobulin A) were measured in digestive fluid. Results Of the 76 patients included, acute gastrointestinal injury was grade I in 41, grade II in 20, grade III in 8, and grade IV in 7. Secretory immunoglobulin A was an independent predictor of grade III acute gastrointestinal injury. When data from patients with grades I and II injury and patients with grades III and IV injury were grouped together, analysis revealed that pH, interleukin 10, and secretory immunoglobulin A were independent predictors of acute gastrointestinal failure. Conclusions Secretory immunoglobulin A was predictive of grade III acute gastrointestinal injury. Digestive fluid markers of injury (pH, interleukin 10, and secretory immunoglobulin A) were predictors of acute gastrointestinal failure. Further study is required to determine if other markers are specific or sensitive for acute gastrointestinal injury.

scholarly journals Funastrain c II: A Cysteine Endopeptidase Purified from the Latex of Funastrum clausum

2004 ◽  
Vol 23 (3) ◽  
pp. 205-215 ◽  
Author(s):  
Susana R. Morcelle ◽  
Sebastián A. Trejo ◽  
Francesc Canals ◽  
Francesc X. Avilés ◽  
Nora S. Priolo
Keyword(s):  
Cysteine Endopeptidase

scholarly journals The participation of phytocystatin TrcC-4 in the activity regulation of EP8, the main prolamin degrading cysteine endopeptidase in triticale seeds

2012 ◽  
Vol 69 (2) ◽  
pp. 131-137 ◽  
Author(s):  
Joanna Szewińska ◽  
Beata Prabucka ◽  
Mariusz Krawczyk ◽  
Marcin Mielecki ◽  
Wiesław Bielawski
Keyword(s):  
Activity Regulation ◽  
Cysteine Endopeptidase

Localization and function ofRhipicephalus (Boophilus) microplusvitellin-degrading cysteine endopeptidase

Parasitology ◽  
2010 ◽  
Vol 137 (12) ◽  
pp. 1819-1831 ◽  
Author(s):  
ADRIANA SEIXAS ◽  
ANDRÉIA B. ESTRELA ◽  
JULIANA C. CEOLATO ◽  
EMERSON G. PONTES ◽  
FLÁVIO LARA ◽  
...  
Keyword(s):  
Fat Body ◽  
Boophilus Microplus ◽  
Basal Region ◽  
Cysteine Endopeptidase ◽  
Endopeptidase Activity ◽  
And Function ◽  
The Tropics ◽  
Different Tissues ◽  
Important Parasite

SUMMARYThe tickRhipicephalus (Boophilus) microplusis an important parasite of cattle in many areas of the tropics. Characterization of molecules involved in mechanisms such as vitellogenesis and embryo development may contribute to a better understanding of this parasite's physiology. The vitellin-degrading cysteine endopeptidase (VTDCE) is the most active enzyme involved in vitellin hydrolysis inR. micropluseggs. Here we show an association between VTDCE and vitellin in an additional site, apart from the active site. Our data also demonstrate cysteine endopeptidase activity in different tissues such as ovary, gut, fat body, salivary gland and female haemolymph, where it is controlled by a physiological inhibitor. InR. microplusfemale gut, VTDCE is localized in areas of protein synthesis and trafficking with the underlying haemolymph. VTDCE is also localized in the ovary basal region, in vesicle membranes of ovary pedicel cells and in oocyte cytosol. These results suggest that VTDCE plays a role in vitellin digestion during tick development.

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