Promoter regions of cysteine endopeptidase genes from legumes confer germination-specific expression in transgenic tobacco seeds

1996 ◽  
Vol 30 (2) ◽  
pp. 321-329 ◽  
Author(s):  
Daisuke Yamauchi ◽  
Yoko Terasaki ◽  
Takashi Okamoto ◽  
Takao Minamikawa
Keyword(s):  
Transgenic Tobacco ◽  
Promoter Regions ◽  
Specific Expression ◽  
Cysteine Endopeptidase ◽  
Tobacco Seeds

Increased seed cytokinin levels in transgenic tobacco influence embryo and seedling development

2002 ◽  
Vol 29 (9) ◽  
pp. 1107 ◽  
Author(s):  
Qing-Hu Ma ◽  
Zhan-Bing Lin ◽  
De-Zhi Fu
Keyword(s):  
Transgenic Tobacco ◽  
Dry Weight ◽  
Polymerase Chain Reaction Analysis ◽  
Single Copy ◽  
Ipt Gene ◽  
Specific Expression ◽  
Tobacco Seeds ◽  
Cell Layers ◽  
Polymerase Chain ◽  
Transgenic Embryos

Seed-specific expression of a heterologous vicilin-isopentenyl transferase (ipt) gene has been determined in transgenic tobacco lines 7 and 16. Genetic analysis of self-fertilized progeny showed that a single copy of the vicilin-ipt gene had been integrated, and T2 progeny had become homozygous for the transgene. Stable inheritance of the vicilin-ipt gene in T3 progeny was verified by polymerase chain reaction analysis. Seed cytokinin levels were 2-3-fold higher than control levels. Cytological analyses revealed a significant increase in the number of plerome cell layers and enlargement of diameter in transgenic embryos compared with controls. Dry weight of mature seeds and subsequent seedling growth was increased significantly. This correlated with the level of vicilin-ipt overexpression and increased cytokinin levels in transgenic tobacco seeds. The results suggest a crucial role for cytokinins in regulation of tobacco embryo development.

Tissue-specific expression of an oat 12S seed glubulin gene in developing tobacco seeds: differential mRNA and protein accumulation

1994 ◽  
Vol 26 (1) ◽  
pp. 203-210 ◽  
Author(s):  
Roland Schubert ◽  
Reinhard Panitz ◽  
Renate Manteuffel ◽  
Istvan Nagy ◽  
Ulrich Wobus ◽  
...  
Keyword(s):  
Protein Accumulation ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Tobacco Seeds

scholarly journals Characteristics of the promoters derived from the single-stranded DNA components of Milk vetch dwarf virus in transgenic tobacco

2005 ◽  
Vol 86 (6) ◽  
pp. 1851-1860 ◽  
Author(s):  
Naomi Shirasawa-Seo ◽  
Yoshitaka Sano ◽  
Shigeo Nakamura ◽  
Taka Murakami ◽  
Shigemi Seo ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Cell Types ◽  
Gus Expression ◽  
Cauliflower Mosaic Virus ◽  
Dwarf Virus ◽  
Promoter Regions ◽  
Meristematic Tissue ◽  
Link Protein ◽  
Gus Reporter ◽  
Milk Vetch Dwarf Virus

Predicted promoter regions of Milk vetch dwarf virus (MDV) components (C1–C11) were isolated and fused with a β-glucuronidase (GUS) reporter gene and the characteristics of the promoters were examined. In transgenic tobacco calli, promoters of MDV C4 (encoding a cell-cycle link protein), C5 and C7 (both encoding unknown proteins), C6 (encoding a nuclear-shuttle protein) and C8 (encoding a movement protein) generated a stronger level of GUS expression than the Cauliflower mosaic virus 35S RNA promoter (P35S). In leaves of transgenic tobacco plants, the promoters of C5 and C8 conferred a level of GUS activity comparable to that of P35S. Histochemical GUS analysis showed that the promoters of C4–C9, the latter encoding a capsid protein, were active in phloem and meristematic tissue. The promoter of C8 was also active in mesophyll and cortex cell types. A low level of activity was found for the promoters of C11, which encodes a master replication-initiator protein (Rep), and C1, C2, C3 and C10, which encode additional Reps, in both transgenic tobacco calli and plants.

Tissue-specific expression directed by an Arabidopsis thaliana pre-ferredoxin promoter in transgenic tobacco plants

1990 ◽  
Vol 14 (4) ◽  
pp. 491-499 ◽  
Author(s):  
Oscar Vorst ◽  
Frans van Dam ◽  
Renske Oosterhoff-Teertstra ◽  
Sjef Smeekens ◽  
Peter Weisbeek
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Specific Expression ◽  
Tobacco Plants ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Identification and Characterization of the Potential Promoter Regions of 1031 Kinds of Human Genes

2001 ◽  
Vol 11 (5) ◽  
pp. 677-684
Author(s):  
Yutaka Suzuki ◽  
Tatsuhiko Tsunoda ◽  
Jun Sese ◽  
Hirotoshi Taira ◽  
Junko Mizushima-Sugano ◽  
...  
Keyword(s):  
Large Scale ◽  
Expression Patterns ◽  
Cpg Islands ◽  
Genomic Sequences ◽  
Cdna Libraries ◽  
Promoter Regions ◽  
Specific Expression ◽  
Link Type ◽  
Potential Promoter ◽  
E Boxes

To understand the mechanism of transcriptional regulation, it is essential to identify and characterize the promoter, which is located proximal to the mRNA start site. To identify the promoters from the large volumes of genomic sequences, we used mRNA start sites determined by a large-scale sequencing of the cDNA libraries constructed by the “oligo-capping” method. We aligned the mRNA start sites with the genomic sequences and retrieved adjacent sequences as potential promoter regions (PPRs) for 1031 genes. The PPR sequences were searched to determine the frequencies of major promoter elements. Among 1031 PPRs, 329 (32%) contained TATA boxes, 872 (85%) contained initiators, 999 (97%) contained GC box, and 663 (64%) contained CAAT box. Furthermore, 493 (48%) PPRs were located in CpG islands. This frequency of CpG islands was reduced in TATA+/Inr+PPRs and in the PPRs of ubiquitously expressed genes. In the PPRs of the CGM2 gene, the DRA gene, and theTM30pl genes, which showed highly colon specific expression patterns, the consensus sequences of E boxes were commonly observed. The PPRs were also useful for exploring promoter SNPs.[The nucleotide sequences described in this paper have been deposited in the DDBJ, EMBL, and GenBank data libraries under accession nos.AU098358–AU100608.]

Genome-wide identification and expression analysis of aquaporin gene family related to abiotic stress in watermelon

Genome ◽  
2019 ◽  
Vol 62 (10) ◽  
pp. 643-656 ◽  
Author(s):  
Yong Zhou ◽  
Junjie Tao ◽  
Golam Jalal Ahammed ◽  
Jingwen Li ◽  
Youxin Yang
Keyword(s):  
Expression Analysis ◽  
Developmental Stages ◽  
Plant Responses ◽  
Distribution Analysis ◽  
Cis Elements ◽  
Promoter Regions ◽  
Specific Expression ◽  
Promoter Sequences ◽  
Genome Wide ◽  
Aquaporin Gene

The plant aquaporins (AQPs) are highly conserved integral membrane proteins that participate in multiple developmental processes and responses to various stresses. In this study, a total of 35 AQP genes were identified in the watermelon genome. The phylogenetic analysis showed that these AQPs can be divided into five types, including 16 plasma membrane intrinsic proteins (PIPs), eight tonoplast intrinsic proteins (TIPs), eight nodulin 26-like intrinsic proteins (NIPs), two small basic intrinsic proteins (SIPs), and one uncategorized X intrinsic protein (XIP). A number of cis-elements related to plant responses to hormones and stresses were detected in the promoter sequences of ClAQP genes. Chromosome distribution analysis revealed that the genes are unevenly distributed on eight chromosomes, with chromosomes 1 and 4 possessing the most genes. Expression analysis at different developmental stages in flesh and rind indicated that most of ClAQPs have tissue-specific expression. Meanwhile, some other AQP genes showed differential expression in response to cold, salt, and ABA treatments, which is consistent with the organization of the stress-responsive cis-elements detected in the promoter regions. Our results lay a foundation for understanding the specific functions of ClAQP genes to help the genetic improvement of watermelon.

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