scholarly journals Breeding of a new six-rowed hulled barley cultivar "Harushirane" with proanthocyanidin-free gene

2013 ◽  
Vol 15 (3) ◽  
pp. 105-109
Author(s):  
Emiko Aoki ◽  
Takuji Tonooka ◽  
Toji Yoshioka ◽  
Naoyuki Kawada ◽  
Megumi Yoshida
Keyword(s):  
Barley Cultivar ◽  
Free Gene ◽  
Hulled Barley

scholarly journals “Shirayuri Nijo”, a new two-rowed hulled barley cultivar for food with proanthocyanidin-free gene, moderate tolerance to pre-harvest sprouting and high-yield

2021 ◽  
Author(s):  
Masato Taira ◽  
Mikiko Yanaka ◽  
Kazuhiro Nakamura ◽  
Tetsufumi Sakai ◽  
Hitoshi Matsunaka ◽  
...  
Keyword(s):  
High Yield ◽  
Barley Cultivar ◽  
Free Gene ◽  
Hulled Barley

scholarly journals “Haneumamochi”, a six-rowed hulled barley cultivar with a new waxy allele

2018 ◽  
Vol 20 (2) ◽  
pp. 133-137
Author(s):  
Masako Seki ◽  
Takashi Nagamine ◽  
Hideyuki Aoki ◽  
Osamu Yamaguchi ◽  
Seiji Ito ◽  
...  
Keyword(s):  
Barley Cultivar ◽  
Hulled Barley

Antioxidant Activity of Hericium erinaceum Grown on Hulled Barley as New Substitute Tea

2017 ◽  
Vol 23 (1) ◽  
pp. 22-29
Author(s):  
Young Min Lee ◽  
Kyoung Hee Seo ◽  
Mithun Ghosh ◽  
Jin Seong Kim ◽  
Beong Ou Lim
Keyword(s):  
Antioxidant Activity ◽  
Hericium Erinaceum ◽  
Hulled Barley

scholarly journals Genome Assembly of the Canadian Two-row Malting Barley Cultivar AAC Synergy

2021 ◽  
Author(s):  
Wayne Xu ◽  
James R Tucker ◽  
Wubishet A Bekele ◽  
Frank M You ◽  
Yong-Bi Fu ◽  
...  
Keyword(s):  
Reference Genome ◽  
Single Copy ◽  
Barley Cultivar ◽  
Malting Barley ◽  
Orthologous Genes ◽  
Hordeum Vulgare L ◽  
Chromosome Conformation ◽  
Mate Pair ◽  
Genome Assemblies ◽  
First Time

Abstract Barley (Hordeum vulgare L.) is one of the most important global crops. The six-row barley cultivar Morex reference genome has been used by the barley research community worldwide. However, this reference genome can have limitations when used for genomic and genetic diversity analysis studies, gene discovery, and marker development when working in two-row germplasm that is more common to Canadian barley. Here we assembled, for the first time, the genome sequence of a Canadian two-row malting barley, cultivar AAC Synergy. We applied deep Illumina paired-end reads, long mate-pair reads, PacBio sequences, 10X chromium linked read libraries, and chromosome conformation capture sequencing (Hi-C) to generate a contiguous assembly. The genome assembled from super-scaffolds had a size of 4.85 Gb, N50 of 2.32 Mb and an estimated 93.9% of complete genes from a plant database (BUSCO, benchmarking universal single-copy orthologous genes). After removal of small scaffolds (< 300 Kb), the assembly was arranged into pseudomolecules of 4.14 Gb in size with seven chromosomes plus unanchored scaffolds. The completeness and annotation of the assembly were assessed by comparing it with the updated version of six-row Morex and recently released two-row Golden Promise genome assemblies.

scholarly journals Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jasmine M. Hershewe ◽  
Katherine F. Warfel ◽  
Shaelyn M. Iyer ◽  
Justin A. Peruzzi ◽  
Claretta J. Sullivan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Synthetic Biology ◽  
Membrane Protein ◽  
Membrane Vesicles ◽  
Processing Methods ◽  
Glycoprotein Synthesis ◽  
On Demand ◽  
Free Gene ◽  
Membrane Bound

AbstractCell-free gene expression (CFE) systems from crude cellular extracts have attracted much attention for biomanufacturing and synthetic biology. However, activating membrane-dependent functionality of cell-derived vesicles in bacterial CFE systems has been limited. Here, we address this limitation by characterizing native membrane vesicles in Escherichia coli-based CFE extracts and describing methods to enrich vesicles with heterologous, membrane-bound machinery. As a model, we focus on bacterial glycoengineering. We first use multiple, orthogonal techniques to characterize vesicles and show how extract processing methods can be used to increase concentrations of membrane vesicles in CFE systems. Then, we show that extracts enriched in vesicle number also display enhanced concentrations of heterologous membrane protein cargo. Finally, we apply our methods to enrich membrane-bound oligosaccharyltransferases and lipid-linked oligosaccharides for improving cell-free N-linked and O-linked glycoprotein synthesis. We anticipate that these methods will facilitate on-demand glycoprotein production and enable new CFE systems with membrane-associated activities.

PSIII-16 Growth Performance of Weaned Pigs Fed Barley Differing in Fermentable Starch and Fiber Profile

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 175-176
Author(s):  
Joaquin J Sanchez Zannatta ◽  
L F Wang ◽  
Eduardo Beltranena ◽  
Aaron D Beattie ◽  
Rex N Newkirk ◽  
...  
Keyword(s):  
Energy Source ◽  
Growth Performance ◽  
Steam Explosion ◽  
Phase 1 ◽  
Average Daily Gain ◽  
Barley Grain ◽  
Gut Health ◽  
Weaned Pigs ◽  
Cereal Grain ◽  
Hulled Barley

Abstract Barley grain containing more fermentable starch or fiber might be an attractive energy source in weaned pig diets due to benefits on gut health. Barley rapidly-fermentable carbohydrates may serve as prebiotic and slowly-fermentable fiber may decrease diarrhea in weaned pigs. Steam-explosion processing may disrupt the fiber matrix of hulls, increasing slowly-fermentable fiber of barley. To explore, 220 pigs were fed 1 of 5 diets containing 60% cereal grain: 1) low-fermentable hulled barley (LFB); 2) LFB steam-exploded (LFB-E; 1.2 MPa, 120 s); 3) high β-glucan (10% DM) hull-less barley (HFB); 4) high amylose (17% DM) hull-less barley (HFA); or 5) low-fermentable wheat (LFW). Diets were fed starting 1-week post-weaning and formulated to provide 2.4 and 2.3 Mcal net energy (NE)/kg, 5.5 and 5.1 g standardized ileal digestible lysine/Mcal NE for phase 1 (day 1–14) and phase 2 (day 15–35), respectively. For the entire trial (day 1–35), average daily feed intake (ADFI) and average daily gain (ADG) of pigs did not differ among diets. Gain:feed (G:F) did not differ between LFB and LFW diets, but steam-explosion of hulled barley reduced (P < 0.05) G:F. Feces consistency did not differ between LFB and LFW diets, but was better (P < 0.05) for LFB than HFB, HFA and LFB-E diets. For phase 1, G:F of pigs was lower (P < 0.05) for LFB-E diet than LFW diet. For days 22–28, LFB-E diet had greater (P < 0.01) ADFI than HFA diet and tended (P = 0.09) to have a greater ADG than HFB diet. In conclusion, hulled or hull-less barley grain replaced wheat grain without affecting growth performance in weaned pigs. Hulled barley increased feces consistency. Steam-explosion of hulled barley did not increase growth performance of weaned pigs. Barley grain is an attractive energy source for weaned pigs for managing growth and feces consistency.

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