scholarly journals Killing of Torulopsis glabrata by Saccharomyces cerevisiae Killer Factor

1976 ◽  
Vol 9 (2) ◽  
pp. 352-354 ◽  
Author(s):  
H. Bussey ◽  
N. Skipper
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Torulopsis Glabrata

Surface Structures in Budding Cells of Torulopsis Giabrata

1969 ◽  
Vol 27 ◽  
pp. 390-391
Author(s):  
Mercedes R. Edwards ◽  
Stanley Holt
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fine Structure ◽  
Yeast Species ◽  
Surface Structures ◽  
Logarithmic Phase ◽  
Torulopsis Glabrata ◽  
Conventional Methods

The fine structure of Saccharomyces cerevisiae has been investigated extensively while relatively little work has been done on other yeast species. The present study compares Torulopsis glabrata with S. cerevisiae. Cells in the logarithmic phase of growth were (a) quickly frozen in Freon 22, cut and replicated in a Balzer's apparatus according to Moor's technique; (b) fixed in glutaraldehyde-osmium, dehydrated, and embedded by conventional methods. Only the illustrations of the surface structures are presented.

scholarly journals Killer toxin of Saccharomyces cerevisiae Y500-4L active against Fleischmann and Itaiquara commercial brands of yeast

1999 ◽  
Vol 30 (3) ◽  
pp. 253-257 ◽  
Author(s):  
Giselle A.M. Soares ◽  
Hélia H. Sato
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Elevated Temperatures ◽  
Killer Toxin ◽  
Toxin Production ◽  
Chromosomal Dna ◽  
Killer Activity ◽  
Killer Yeast ◽  
Torulopsis Glabrata ◽  
Hansenula Anomala ◽  
Killer Yeasts

The strain Saccharomyces cerevisiae Y500-4L, previously selected from the must of alcohol producing plants and showing high fermentative and killer capacities, was characterized according to the interactions between the yeasts and examined for curing and detection of dsRNA plasmids, which code for the killer character. The killer yeast S. cerevisiae Y500-4L showed considerable killer activity against the Fleischmann and Itaiquara commercial brands of yeast and also against the standard killer yeasts K2 (S. diastaticus NCYC 713), K4 (Candida glabrata NCYC 388) and K11 (Torulopsis glabrata ATCC 15126). However S. cerevisiae Y500-4L showed sensitivity to the killer toxin produced by the standard killer yeasts K8 (Hansenula anomala NCYC 435), K9 (Hansenula mrakii NCYC 500), K10 (Kluyveromyces drosophilarum NCYC 575) and K11 (Torulopsis glabrata ATCC 15126). No M-dsRNA plasmid was detected in the S. cerevisiae Y500-4L strain and these results suggest that the genetic basis for toxin production is encoded by chromosomal DNA. The strain S. cerevisiae Y500-4L was more resistant to the loss of the phenotype killer with cycloheximide and incubation at elevated temperatures (40oC) than the standard killer yeast S. cerevisiae K1.

scholarly journals A gene required for RNase P activity in Candida (Torulopsis) glabrata mitochondria codes for a 227-nucleotide RNA with homology to bacterial RNase P RNA

1991 ◽  
Vol 11 (3) ◽  
pp. 1662-1667
Author(s):  
H H Shu ◽  
C A Wise ◽  
G D Clark-Walker ◽  
N C Martin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mitochondrial Dna ◽  
Sequence Homology ◽  
Rnase P ◽  
Micrococcal Nuclease ◽  
Trna Genes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Torulopsis Glabrata ◽  
Nuclease Sensitivity

We have mapped a gene in the mitochondrial DNA of Candida (Torulopsis) glabrata and shown that it is required for 5' end maturation of mitochondrial tRNAs. It is located between the tRNAfMet and tRNAPro genes, the same tRNA genes that flank the mitochondrial RNase P RNA gene in the yeast Saccharomyces cerevisiae. The gene is extremely AT rich and codes for AU-rich RNAs that display some sequence homology with the mitochondrial RNase P RNA from S. cerevisiae, including two regions of striking sequence homology between the mitochondrial RNAs and the bacterial RNase P RNAs. RNase P activity that is sensitive to micrococcal nuclease has been detected in mitochondrial extracts of C. glabrata. An RNA of 227 nucleotides that is one of the RNAs encoded by the gene that we mapped cofractionated with this mitochondrial RNase P activity on glycerol gradients. The nuclease sensitivity of the activity, the cofractionation of the RNA with activity, and the homology of the RNA with known RNase P RNAs lead us to propose that the 227-nucleotide RNA is the RNA subunit of the C. glabrata mitochondrial RNase P enzyme.

scholarly journals A gene required for RNase P activity in Candida (Torulopsis) glabrata mitochondria codes for a 227-nucleotide RNA with homology to bacterial RNase P RNA.

1991 ◽  
Vol 11 (3) ◽  
pp. 1662-1667 ◽  
Author(s):  
H H Shu ◽  
C A Wise ◽  
G D Clark-Walker ◽  
N C Martin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mitochondrial Dna ◽  
Sequence Homology ◽  
Rnase P ◽  
Micrococcal Nuclease ◽  
Trna Genes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Torulopsis Glabrata ◽  
Nuclease Sensitivity

We have mapped a gene in the mitochondrial DNA of Candida (Torulopsis) glabrata and shown that it is required for 5' end maturation of mitochondrial tRNAs. It is located between the tRNAfMet and tRNAPro genes, the same tRNA genes that flank the mitochondrial RNase P RNA gene in the yeast Saccharomyces cerevisiae. The gene is extremely AT rich and codes for AU-rich RNAs that display some sequence homology with the mitochondrial RNase P RNA from S. cerevisiae, including two regions of striking sequence homology between the mitochondrial RNAs and the bacterial RNase P RNAs. RNase P activity that is sensitive to micrococcal nuclease has been detected in mitochondrial extracts of C. glabrata. An RNA of 227 nucleotides that is one of the RNAs encoded by the gene that we mapped cofractionated with this mitochondrial RNase P activity on glycerol gradients. The nuclease sensitivity of the activity, the cofractionation of the RNA with activity, and the homology of the RNA with known RNase P RNAs lead us to propose that the 227-nucleotide RNA is the RNA subunit of the C. glabrata mitochondrial RNase P enzyme.

Sign in / Sign up

Export Citation Format

Share Document