scholarly journals Histochemical Observation of Polysaccharides and Lipids on the Developing Anthers of Solanum photeinocarpum

2014 ◽  
Vol 03 (01) ◽  
pp. 27-31
Author(s):  
笑娟 郭
Author(s):  
Seiji Kato

Previously, the author repeatedly confirmed the higher 5’-nucleotidase (5’-Nase) and lower alkaline phoaphatase (ALPase) activities in the wall of lymphatic capillaries reacted with the lead-based method relative to those of blood capillaries. The ALPase, on the other hand, is markedly higher in blood capillaries than in lymphatics. On the basis of these enzyme characteristics, the author has developed a 5’-Nase— ALPase double staining method to differentiate small lymphatics from blood capillaries at the level of the light microcsopy. Furthermore, we applied it to histochemical observation of the lead-containing reaction products of 5’-Nase in lymphatics on the same or adjacent cryostat sections using backscattered electron imaging (BEI) in scanning electron microscope (SEM). This paper presents a new applicability of 5’-Nase histochemistry by BEI-SEM to demonstrate the distribution of lymphatic capillaries in tissue blocks.


2013 ◽  
Vol 31 (1) ◽  
pp. 80
Author(s):  
Shao FU ◽  
Han BAO ◽  
Wei-Dong KE ◽  
Yuan-Ying YE ◽  
You-Hao GUO

1984 ◽  
Vol 34 (5) ◽  
pp. 1003-1016
Author(s):  
Sumio Nakanishi ◽  
Kazuo Hizawa

1989 ◽  
Vol 82 (12) ◽  
pp. 1779-1784
Author(s):  
Shinobu Yamaguchi ◽  
Masaya Takumida ◽  
Tatsuzo Taira ◽  
Michiaki Hiramoto

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