scholarly journals A NEW METHOD FOR HISTOCHEMICAL OBSERVATION OF THE SUBCUTANEOUS CONNECTIVE TISSUE

1960 ◽  
Vol 8 (1) ◽  
pp. 72-73 ◽  
Author(s):  
SYUN HOSODA ◽  
KATSUHIRO NAKAMURA
Keyword(s):  
Connective Tissue ◽  
New Method ◽  
Histochemical Observation ◽  
Subcutaneous Connective Tissue

scholarly journals Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice

2008 ◽  
Vol 19 (1) ◽  
pp. 40-45 ◽  
Author(s):  
Ana Teresa Sant'anna ◽  
Luis Carlos Spolidório ◽  
Lizeti Toledo Oliveira Ramalho
Keyword(s):  
Connective Tissue ◽  
Chronic Inflammation ◽  
Histological Analysis ◽  
Subcutaneous Tissue ◽  
Experimental Period ◽  
Connective Tissues ◽  
Plastic Tube ◽  
Tissue Samples ◽  
Subcutaneous Connective Tissue ◽  
Original Formula

This study performed a histological analysis of the effect of formocresol associated to endotoxin (LPS) in the subcutaneous connective tissue of mice. Ninety mice were randomly assigned to 3 groups (n=30). Each animal received one plastic tube implant containing endotoxin solution (10 mg/mL), formocresol (original formula) or a mixture of endotoxin and formocresol. The endotoxin and formocresol groups served as controls. The periods of analysis were 7, 15 and 30 days. At each experimental period, tissue samples were collected and submitted to routine processing for histological analysis. Endotoxin and formocresol produced necrosis and chronic inflammation at 7 and 15 days. At 30 days, the endotoxin group showed no necrosis, while in the formocresol group necrosis persisted. The formocresol-endotoxin association produced necrosis and chronic inflammation in the same way as observed with formocresol at all experimental periods. In conclusion, formocresol seems not to be able to inactive the toxic effects of endotoxin in connective tissues.

Metastatic Lymphangiosarcoma in a Horse

2002 ◽  
Vol 39 (2) ◽  
pp. 266-268 ◽  
Author(s):  
B. Sanchez ◽  
A. Nieto ◽  
M. A. Ruiz de Leon ◽  
J. Rodríguez ◽  
J. Flores
Keyword(s):  
Connective Tissue ◽  
Basement Membrane ◽  
Lymph Nodes ◽  
Respiratory Distress ◽  
Factor Viii ◽  
Tumor Cells ◽  
Collagen Iv ◽  
Positive Staining ◽  
Tumor Metastases ◽  
Subcutaneous Connective Tissue

A lymphangiosarcoma with metastases was found in a horse that presented with respiratory distress and edema in the ventral thorax and abdomen. The necropsy revealed diffuse edema in the subcutaneous connective tissue. Mediastinal, mesenteric, iliac, and renal lymph nodes were enlarged and white with soft, yellowish necrotic areas. Histologic examination revealed numerous channels and disorganized vessels lined by large polyhedral, polymorphic cells. Tumor metastases were observed in the spleen, lungs, and kidneys. Immunohistochemical evaluation of the tumor cells demonstrated positive staining for factor VIII, vimentin, and keratin. Laminin was scarce, and collagen IV staining was negative, consistent with a discontinuous or absent basement membrane.

scholarly journals On the minute structure of striped muscle, with special reference to a new method of investigation, by means of ‘impressions’ stamped in collodion

1891 ◽  
Vol 49 (296-301) ◽  
pp. 287-303 ◽  
Keyword(s):  
Connective Tissue ◽  
Special Reference ◽  
New Method ◽  
Uniform Thickness ◽  
Striped Muscle

Curiously enough many of the early microscopists—Schwann for instance—recognised that the fibrils of a muscle are not simply threads of uniform thickness, like those of connective tissue: they were able to demonstrate their varicose character, even with the imperfect lenses at their command.

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